Simultaneous production of poly-γ-glutamic acid and 2,3-butanediol by a newly isolated Bacillus subtilis CS13

Wang, Dexin; Kim, Hyangmi; Lee, Sungbeom; Kim, Dae-Hyuk; Joe, Min-Ho

doi:10.1007/s00253-020-10755-0

Cited by 10 publications

(11 citation statements)

References 62 publications

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“…Previous research has found that sucrose, ammonium citrate, and MgSO 4 •7H 2 O exert significant effects on 2,3-BD production [18]. In this study, the face-centered central composite design (FCCD) was employed to optimize the three most significant variables for further enhancing 2,3-BD production.…”

Section: Rsm Experimental Designmentioning

confidence: 99%

“…+ enhances glutamate metabolism conversion of NADPH to NADP + , which subsequently increases NADH accumulation and promotes 2,3-BD synthesis [18,21]. It is reported that the yield of 2,3-BD can be increased by addition of different organic acid, and ammonium citrate acts as an intermediate metabolite to promote the formation of 2,3-BD [14,22].…”

Section: Rsm Design For 23-bd Productionmentioning

confidence: 99%

“…B. subtilis CS13 was previously isolated in our laboratory for the co-production of 2,3-BD and poly-γ-glutamic acid (γ-PGA) [18]. In this study, the fermentation medium for 2,3-BD production was optimized using response surface methodology (RSM).…”

Section: Introductionmentioning

confidence: 99%

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Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13

Wang

Lee

et al. 2021

Biotechnol Biofuels

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Background Bacillus subtilis CS13 was previously isolated for 2,3-butanediol (2,3-BD) and poly-γ-glutamic acid (γ-PGA) co-production. When culturing this strain without L-glutamic acid in the medium, 2,3-BD is the main metabolic product. 2,3-BD is an important substance and fuel with applications in the chemical, food, and pharmaceutical industries. However, the yield and productivity for the B. subtilis strain should be improved for more efficient production of 2,3-BD. Results The medium composition, which contained 281.1 g/L sucrose, 21.9 g/L ammonium citrate, and 3.6 g/L MgSO4·7H2O, was optimized by response surface methodology for 2,3-BD production using B. subtilis CS13. The maximum amount of 2,3-BD (125.5 ± 3.1 g/L) was obtained from the optimized medium after 96 h. The highest concentration and productivity of 2,3-BD were achieved simultaneously at an agitation speed of 500 rpm and aeration rate of 2 L/min in the batch cultures. A total of 132.4 ± 4.4 g/L 2,3-BD was obtained with a productivity of 2.45 ± 0.08 g/L/h and yield of 0.45 g2,3-BD/gsucrose by fed-batch fermentation. The meso-2,3-BD/2,3-BD ratio of the 2,3-BD produced by B. subtilis CS13 was 92.1%. Furthermore, 89.6 ± 2.8 g/L 2,3-BD with a productivity of 2.13 ± 0.07 g/L/h and yield of 0.42 g2,3-BD/gsugar was achieved using molasses as a carbon source. Conclusions The production of 2,3-BD by B. subtilis CS13 showed a higher concentration, productivity, and yield compared to the reported generally recognized as safe 2,3-BD producers. These results suggest that B. subtilis CS13 is a promising strain for industrial-scale production of 2,3-BD.

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Section: Rsm Experimental Designmentioning

confidence: 99%

Section: Rsm Design For 23-bd Productionmentioning

confidence: 99%

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Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13

Wang

Lee

et al. 2021

Biotechnol Biofuels

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“…The alsS gene encodes α-acetolactate synthase, which condenses two molecules of pyruvate to form acetolactate. Acetolactate is converted to acetoin by the action of acetolactate decarboxylase, followed by the synthesis of 2,3-BD [ 42 ]. In B. subtilis , after disruption of the alsS gene, the strain does not grow unless valine or isoleucine is added.…”

Section: Resultsmentioning

confidence: 99%

Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid

Wang

Zhou

et al. 2022

Microb Cell Fact

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Background Poly γ-glutamic acid (γ-PGA) is a promising biopolymer for various applications. For glutamic acid-independent strains, the titer of γ-PGA is too low to meet the industrial demand. In this study, we isolated a novel γ-PGA-producing strain, Bacillus tequilensis BL01, and multiple genetic engineering strategies were implemented to improve γ-PGA production. Results First, the one-factor-at-a-time method was used to investigate the influence of carbon and nitrogen sources and temperature on γ-PGA production. The optimal sources of carbon and nitrogen were sucrose and (NH4)2SO4 at 37 °C, respectively. Second, the sucA, gudB, pgdS, and ggt genes were knocked out simultaneously, which increased the titer of γ-PGA by 1.75 times. Then, the titer of γ-PGA increased to 18.0 ± 0.3 g/L by co-overexpression of the citZ and pyk genes in the mutant strain. Furthermore, the γ-PGA titer reached 25.3 ± 0.8 g/L with a productivity of 0.84 g/L/h and a yield of 1.50 g of γ-PGA/g of citric acid in fed-batch fermentation. It should be noted that this study enables the synthesis of low (1.84 × 105 Da) and high (2.06 × 106 Da) molecular weight of γ-PGA by BL01 and the engineering strain. Conclusion The application of recently published strategies to successfully improve γ-PGA production for the new strain B. tequilensis BL01 is reported. The titer of γ-PGA increased 2.17-fold and 1.32-fold compared with that of the wild type strain in the flask and 5 L fermenter. The strain shows excellent promise as a γ-PGA producer compared with previous studies. Meanwhile, different molecular weights of γ-PGA were obtained, enhancing the scope of application in industry.

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“…While increasing the glutamate concentration in the media can increase γ‐PGA synthesis, this procedure also raises the overall cost of γ‐PGA bioproduction (Luo et al., 2016). Therefore, ongoing research aims to enhance the production of γ‐PGA through the isolation of new strains, medium optimization (Wang, Kim, et al., 2020), genetic engineering of metabolic pathways (Gao et al., 2019), and induction of environmental shocks to upregulate the γ‐PGA synthetase operon (Song et al., 2020; Tang et al., 2016). Previously, genetically modified organisms have been employed to overcome the constraints of the metabolic flux between substrates and products.…”

Section: Introductionmentioning

confidence: 99%

Electrofermentation increases concentration of poly γ‐glutamic acid in Bacillus subtilis biofilms

Adilkhanova,

Ormantayeva,

Kaziullayeva

et al. 2024

Microbial Biotechnology

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Fluctuations in redox conditions in bioprocesses can alter the end‐products, reduce their concentration, and lengthen the process time. Electrofermentation enables rapid metabolic modulation of biosynthesis and allows control of redox imbalances in biofilm‐based fermentation processes. In this study, electrofermentation is used to boost the production of the bacterial biopolymer poly‐γ‐glutamic acid (γ‐PGA) from Bacillus subtilis ATCC 6051. When compared to control experiments (3.3 ± 0.99 g L−1), the application of an electrode potential E = 0.4 V versus Ag/AgCl results in a more than two‐fold increase in the production of γ‐PGA (9.13 ± 1.4 g L−1). Using an engineered B. subtilis strain, in which γ‐PGA production is driven by isopropyl β‐d‐1‐thiogalactopyranoside, electrofermentation improves polymer concentrations from 15.4 ± 1.5 to 23.1 ± 1.6 versus g L−1. These results confirm that electrofermentation conditions can be adopted to increase the concentration of γ‐PGA and perhaps other extracellular biopolymers in industrial strains.

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Simultaneous production of poly-γ-glutamic acid and 2,3-butanediol by a newly isolated Bacillus subtilis CS13

Cited by 10 publications

References 62 publications

Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13

Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13

Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid

Electrofermentation increases concentration of poly γ‐glutamic acid in Bacillus subtilis biofilms

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