Simultaneous multiresidue determination of metronidazole and spiramycin in fish muscle using high performance liquid chromatography with UV detection

Maher, Hadir M.; Youssef, Rasha M.; Khalil, Riad H.; El-Bahr, Sabry M.

doi:10.1016/j.jchromb.2008.10.033

Cited by 80 publications

(44 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…37 Among these substances, neospiramycin was identied as the major transformation product of spiramycin 32,38 reaching up to 5.3 AE 0.4 mg L À1 in spiramycin production wastewater.…”

Section: 36mentioning

confidence: 99%

Evaluation of residual antibacterial potency in antibiotic production wastewater using a real-time quantitative method

Zhang

Yang

Liu

2015

Environ. Sci.: Processes Impacts

View full text Add to dashboard Cite

a While antibiotic pollution has attracted considerable attention due to its potential in promoting the dissemination of antibiotic resistance genes in the environment, the antibiotic activity of their related substances has been neglected, which may underestimate the environmental impacts of antibiotic wastewater discharge. In this study, a real-time quantitative approach was established to evaluate the residual antibacterial potency of antibiotics and related substances in antibiotic production wastewater (APW) by comparing the growth of a standard bacterial strain (Staphylococcus aureus) in tested water samples with a standard reference substance (e.g. oxytetracycline). Antibiotic equivalent quantity (EQ) was used to express antibacterial potency, which made it possible to assess the contribution of each compound to the antibiotic activity in APW. The real-time quantitative method showed better repeatability (Relative Standard Deviation, RSD 1.08%) compared with the conventional fixed growth time method (RSD 5.62-11.29%). And its quantification limits ranged from 0.20 to 24.00 mg L À1, depending on the antibiotic. We applied the developed method to analyze the residual potency of water samples from four APW treatment systems, and confirmed a significant contribution from antibiotic transformation products to potent antibacterial activity. Specifically, neospiramycin, a major transformation product of spiramycin, was found to contribute 13.15-22.89% of residual potency in spiramycin production wastewater. In addition, some unknown related substances with antimicrobial activity were indicated in the effluent. This developed approach will be effective for the management of antibacterial potency discharge from antibiotic wastewater and other waste streams. Environmental impactAntibiotic pollution from antibiotic production wastewater has caused increasing concern worldwide. Previous studies have focused on the discharge of antibiotics by determining their concentrations. However, some antibiotic related substances in effluents still possess antibacterial activity, which might induce emergence and proliferation of antibiotic resistant bacteria and genes in the environment. Therefore, it is important to investigate residual antibacterial potency in antibiotic production wastewater with high concentrations of antibiotics and related substances. In this study, a real-time quantitative method was developed and applied for the analysis of residual antibacterial potency of antibiotic production wastewater. The results showed that spiramycin production wastewater contained transformation products with high potency. This method made it possible to assess the contribution of each compound and should be a useful tool for environmental risk management for providing the basis for identication of transformation products with antibacterial activity.

show abstract

“…37 Among these substances, neospiramycin was identied as the major transformation product of spiramycin 32,38 reaching up to 5.3 AE 0.4 mg L À1 in spiramycin production wastewater.…”

Section: 36mentioning

confidence: 99%

Evaluation of residual antibacterial potency in antibiotic production wastewater using a real-time quantitative method

Zhang

Yang

Liu

2015

Environ. Sci.: Processes Impacts

View full text Add to dashboard Cite

show abstract

“…(2-methyl-5-nitroimidazole-1-ethanol, MTZ) is a nitroimidazole antibiotic, which is used particularly for protozoal and an aerobic bacterial infections [9,19].Several methods have been stated for the measurement of metronidazole including spectrophotometry [20][21][22][23][24], high performance liquid chromatography (HPLC) [25][26][27][28] and gas chromatography [29,30]. MTZ can also be electrochemically investigated by the reduction of its nitro group (Scheme 1).…”

Section: Metronidazolementioning

confidence: 99%

Sensitive determination of metronidazole based on Graphene-TiO2 modified glassy carbon electrode in human serum and urine samples

et al. 2015

View full text Add to dashboard Cite

In the present work, a new Graphene-TiO2 (GR-TiO2) modified glassy carbon electrode (GCE) is suggested for sensitive electrochemical determination of metronidazole (MTZ). Electrochemical studies revealed that GR-TiO2 nanoparticles increased the efficiency of electron transfer kinetics by increasing the available surface area of the electrode and charge mobility between MTZ and GR-TiO2 modified electrode. Compared to bare GCE, the modified electrode greatly enhanced the reduction signal of MTZ. The electrochemical behaviour of the modified electrode and the electrochemical reduction of MTZ were investigated with electrochemical impedance spectroscopy, cyclic voltammetry and differential pulse voltammetry techniques. The charge transfer coefficient (α) was calculated to be 0.694. Under optimized conditions, the linear concentration range and detection limit of MTZ were 5.0×10 -7 to 2.5×10 -5 M -1 and 5.4×10 -8 (S/N = 3), respectively. Finally, this sensing method was successfully applied for the determination of MTZ in human blood serum and urine samples.

show abstract

“…In order for a drug residue to be positively confirmed, it must meet certain criteria that have been outlined in general guidelinesῌ 7 . In spiked salmon extracts, the retention time and the ratio between the relative abundance of the selected product ions for each compound fell within the tolerance limits.…”

Section: Protonated Molecules [Mῃh]mentioning

confidence: 99%

“…Analytical methods for detecting DMZ, MNZ and RNZ residues in trout and tilapia have been proposed using UV detection 6), 7) . Smith et al 9) have reported success using LC-ion trap MS strategies for detecting MNZ in salmon, trout, catfish and tilapia; however, no other methods have been found for the simultaneous determination of DMZ, MNZ and RNZ in salmon by LC-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Determination of Dimetridazole, Metronidazole and Ronidazole in Salmon and Honey by Liquid Chromatography Coupled with Tandem Mass Spectrometry

Takeba¹,

Sasamoto²,

Kusano³

et al. 2011

Journal of the Food Hygienics Society of Japan

View full text Add to dashboard Cite

A liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was developed to determine the residues of dimetridazole (DMZ), metronidazole (MNZ) and ronidazole (RNZ) in salmon and honey. These compounds were extracted with ethyl acetate from samples and cleaned up using a silica solid phase extraction (SPE) cartridge. These compounds were determined by reversed-phase LC using a C18 column with distilled waterῌmethanol as the mobile phase, and MS detection in the positive mode by applying selected reaction monitoring (SRM). DMZ-d 3 , MNZ-13 C 2 , 15 N 2 and RNZ-d 3 were used as internal standards. The method was validated in salmon and honey spiked with these compounds at 0.4ῌ2 ῌg/kg, and average recoveries were in the range of 91.2ῌ107.0ῌ. Repeatability was 1.7ῌ17.1ῌ and intermediate precision was less than 20ῌ. The detection limits of DMZ, MNZ and RNZ in salmon and honey were 0.05ῌ0.2 ῌg/kg. The method was applied to 3 salmon and 20 honey samples. The concentrations of these compounds in all samples were lower than the detection limits established by the Ministry of Health, Labour and Welfare in Japan.

show abstract

Simultaneous multiresidue determination of metronidazole and spiramycin in fish muscle using high performance liquid chromatography with UV detection

Cited by 80 publications

References 24 publications

Evaluation of residual antibacterial potency in antibiotic production wastewater using a real-time quantitative method

Evaluation of residual antibacterial potency in antibiotic production wastewater using a real-time quantitative method

Sensitive determination of metronidazole based on Graphene-TiO2 modified glassy carbon electrode in human serum and urine samples

Determination of Dimetridazole, Metronidazole and Ronidazole in Salmon and Honey by Liquid Chromatography Coupled with Tandem Mass Spectrometry

Contact Info

Product

Resources

About