Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide

Chodkowska, Karolina A.; Ciecierska, Anna; Majchrzak, Kinga; Ostaszewski, Paweł; Sadkowski, Tomasz

doi:10.3390/nu10121871

Cited by 8 publications

(3 citation statements)

References 83 publications

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“…Furthermore, H 2 O 2 accumulation has been noted in equine sports and diseases, accompanied by cell apoptosis and mitochondrial dysfunction ( Kirschvink et al, 2008 ; Goncalves et al, 2015 ; Ji et al, 2016 ). Our results demonstrated that ESMCs exposed to H 2 O 2 showed elevated oxidative stress, decreased antioxidant capacity, and disrupted cellular energy metabolism, consistent with previous reports ( Chodkowska et al, 2018a ; b ).…”

Section: Discussionsupporting

confidence: 93%

Identification of mulberry leaf flavonoids and evaluating their protective effects on H2O2-induced oxidative damage in equine skeletal muscle satellite cells

Zhang,

Geng,

Cao

et al. 2024

Front. Mol. Biosci.

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Introduction: Horses are susceptible to oxidative stress during strenuous endurance exercise, leading to muscle fatigue and damage. Mulberry leaf flavonoids (MLFs) possess significant antioxidant properties. However, the antioxidant efficacy of MLFs can be influenced by the extraction process, and their impact on H2O2-induced oxidative stress in equine skeletal muscle satellite cells (ESMCs) remains unexplored.Methods: Our study employed three extraction methods to obtain MLFs: ultrasound-assisted extraction (CEP), purification with AB-8 macroporous resin (RP), and n-butanol extraction (NB-EP). We assessed the protective effects of these MLFs on H2O2-induced oxidative stress in ESMCs and analyzed the MLF components using metabolomics.Results: The results revealed that pre-treatment with MLFs dose-dependently protected ESMCs against H2O2-induced oxidative stress. The most effective concentrations were 0.8 mg/mL of CEP, 0.6 mg/mL of RP, and 0.6 mg/mL of NB-EP, significantly enhancing EMSC viability (p < 0.05). These optimized MLF concentrations promoted the GSH-Px, SOD and T-AOC activities (p < 0.05), while reducing MDA production (p < 0.05) in H2O2-induced ESMCs. Furthermore, these MLFs enhanced the gene expression, including Nrf2 and its downstream regulatory genes (TrxR1, GPX1, GPX3, SOD1, and SOD2) (p < 0.05). In terms of mitochondrial function, ESMCs pre-treated with MLFs exhibited higher basal respiration, spare respiratory capacity, maximal respiration, ATP-linked respiration compared to H2O2-induced ESMCs (p < 0.05). Additionally, MLFs enhanced cellular basal glycolysis, glycolytic reserve, and maximal glycolytic capacity (p < 0.05). Metabolomics analysis results revealed significant differences in mulberrin, kaempferol 3-O-glucoside [X-Mal], neohesperidin, dihydrokaempferol, and isobavachalcone among the three extraction processes (p < 0.05).Discussion: Our study revealed that MLFs enhance antioxidant enzyme activity, alleviate oxidative damage in ESMCs through the activation of the Nrf2 pathway, and improve mitochondrial respiration and cell energy metabolism. Additionally, we identified five potential antioxidant flavonoid compounds, suggesting their potential incorporation into the equine diet as a strategy to alleviate exercise-induced oxidative stress.

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Section: Discussionsupporting

confidence: 93%

Identification of mulberry leaf flavonoids and evaluating their protective effects on H2O2-induced oxidative damage in equine skeletal muscle satellite cells

Zhang,

Geng,

Cao

et al. 2024

Front. Mol. Biosci.

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“…However, CON and FLAX remained elevated at 30 min post-exercise on d 0, 30, and 60. This could be explained by RICE containing γ-oryzanol, a mixture of ferulic acid esters that has been shown to decrease lactic acid concentrations after exercise [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Crude Rice Bran Oil and a Flaxseed Oil Blend in Young Horses Engaged in a Training Program

Mowry

Thomson-Parker

Morales

et al. 2022

Animals

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Rice bran oil and flaxseed oil contain omega-3 fatty acids with the potential to reduce post-exercise inflammation and muscle damage. This study measures plasma interleukin-1β and creatine kinase and fatty acid profiles in lightly worked, young horses (Equus caballus) undergoing an exercise test after 60 days (d) of oil consumption, where the oil replaced 25% of concentrate calories. Treatments consisted of CON (no oil), FLAX (flaxseed oil blend), and RICE (crude rice bran oil). Blood was collected pre-exercise, and again at 1 min, 30 min, 24 h, 48 h, and 72 h post-IET. Data were analyzed by repeated measures ANOVA. Plasma creatine kinase activity was not different in CON during the study, greater (p < 0.05) in RICE from pre-exercise to 30 min post-exercise across all exercise tests, and lesser (p < 0.05) in FLAX at 30 min post-exercise on d 30 compared to d 0. Plasma interleukin-1β was greater (p < 0.01) in CON on d 60, but no differences were observed in FLAX and RICE throughout the study. Plasma alpha-linolenic and linoleic acids were greatest (p < 0.05) in FLAX after 30 d of inclusion, while CON horses had greater (p < 0.05) EPA across all exercise tests and DHA after 60 d. These results indicate that 60 d of inclusion of crude rice bran oil or a flaxseed oil blend may benefit lightly worked, young horses by reducing training-program-related increases in interleukin-1β, while a flaxseed oil blend may reduce exercise-induced increases in creatine kinase. Additionally, the flaxseed oil blend has the potential to increase plasma omega-3 and omega-6 fatty acids. Replacing 25% of concentrate calories with flaxseed or rice bran oil has potential benefits for young horses in training.

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“…W oparciu o wyniki badań wykorzystujących komórki tkanki mięśniowej poprzecznie prążkowanej, pobranej od młodego konia, ustalono również, że gamma-oryzanol przyspiesza możliwości regeneracyjne. Wzrost szybkości odbudowy mięśni po wysiłku fizycznym skorelowany jest ze wspomaganiem aktywności komórek satelitarnych niezbędnych dla prawidłowego procesu odbudowy włókien mięśniowych uszkadzanych podczas intensywnego treningu [49].…”

Section: Aktywność Biologiczna Wynikająca Z Potencjału Przeciwutleniającego Gamma-oryzanoluunclassified

Gamma-oryzanol jako przykład kompleksu estrów fitosteroli i kwasu ferulowego o szerokiej aktywności biologicznej

Młodzińska-Michta

Korzeniowska

2021

Postepy Biochem

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Rośliny są naturalnymi laboratoriami, których wtórny metabolizm produkuje całe bogactwo związków chemicznych o ogromnym potencjale terapeutycznym. Olej ekstrahowany z otrębów ryżowych, stanowiących frakcję uboczną w procesie obróbki brązowego ryżu, obfituje w substancje biologicznie cenne. Jednym z głównych składników oleju ryżowego jest gamma-oryzanol stanowiący mieszaninę będących pochodnymi fitosteroli lub triterpenów oraz kwasu ferulowego. Substraty te charakteryzują się szeroką aktywnością biologiczną ściśle skorelowaną z ich właściwościami chemicznymi. Kluczowe w kontekście poprawy fizjologii i funkcjonowania organizmu są hipocholesterolemiczne oraz antyoksydacyjne właściwości tych związków. Z tego względu coraz więcej badań skupia się na wykorzystywaniu gamma-oryzanolu w terapiach wielu chorób przewlekłych, a nawet jako obiecującego, niefarmakologicznego środka leczniczego wspomagającego leczenie COVID-19 u osób cierpiących na nadwagę. W pracy scharakteryzowano strukturę chemiczną i działanie gamma-oryzanolu w oparciu o aktywność biologiczną estrów fitosteroli i kwasu ferulowego będących składnikami tej złożonej mieszaniny. Ponadto, opisano przykłady oddziaływania gamma oryzanolu na niektóre procesy fizjologiczne w organizmie człowieka i zwierząt.

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Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide

Cited by 8 publications

References 83 publications

Identification of mulberry leaf flavonoids and evaluating their protective effects on H2O2-induced oxidative damage in equine skeletal muscle satellite cells

Identification of mulberry leaf flavonoids and evaluating their protective effects on H2O2-induced oxidative damage in equine skeletal muscle satellite cells

Effects of Crude Rice Bran Oil and a Flaxseed Oil Blend in Young Horses Engaged in a Training Program

Gamma-oryzanol jako przykład kompleksu estrów fitosteroli i kwasu ferulowego o szerokiej aktywności biologicznej

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