Simultaneous determination of seven main alkaloids of Chelidonium majus L. by ultra‐performance LC with photodiode‐array detection

Abstract

A simple and rapid method for the simultaneous determination of seven isoquinoline alkaloids, protopine, chelidonine, coptisine, stylopine, sanguinarine, berberine, and chelerythrine, in Chelidonium majus L. (Ch. majus) samples by ultra-performance LC method with photodiode array detection is described. The baseline separation of these compounds was performed with (A) acetonitrile-(B) ammonium acetate (10 mM, adjusted to pH 3.0 with acetic acid) as the mobile phase using a C18 RP column (2.1x100 mm, 1.7 microm). Optimized conditions resulted in excellent peak shapes. The seven alkaloids were completely separated within 20 min. Good linear behaviors (r > or = 0.9992) over the investigated concentration ranges were observed for all the analytes. Validation proved the repeatability of the method was good and recovery was satisfactory. The validated method was successfully applied for 20 batches of Ch. majus. These results demonstrated that the ultra-performance LC photodiode array method proposed was very useful in the analysis and quality control of Ch. majus.

Comparative Fingerprint and Extraction Yield of Medicinal Herb Phenolics with Hepatoprotective Potential, as Determined by UV-Vis and FT-MIR Spectroscopy

“…It is rich in alkaloids (Gu et al, 2010), mainly chelidonin, a naphtofenantridin derivative which stimulates the enzymatic production in liver and pancreas, it is colecystokinetic and antispastic (Gilca et al, 2010;Hriscu et al, 1980). It was demonstrated experimentally to be antiinflamator, anticancerigenic şi antimicrobian (Vavreckova et al, 1996) being recommended in cyrosis and chronic hepatitis therapy.…”

HPLC-DAD determination of berberine, chelerythrine and sanguinarine in the mexican prickly poppy (Argemone mexicana L. Papaveraceae), a medicinal plant

“…A flow rate of 1.2 mL min -1 provided an optimal signal-to-noise ratio, as well as shorter retention times than previously reported. 9,[16][17] It is important to note that variations in retention times were within the 0.01 min order (RSD lower than 2%). Hence, this method allows performing a complete alkaloid analysis from raw mixtures in fewer than 5 min, reducing in this way the cost of analysis.…”

“…This is notably shorter time than those in previous reports. [16][17][18][19] Moreover; the method has been validated according to standards in the USP and ICH.…”

Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants

“…High-performance liquid chromatography (HPLC) methods [25][26][27] have previously been used to separate CHE and SAN, but HPLC often need time-consuming sample preparation steps, large solvent consumption and long analysis time. As an improvement, an ultra-performance liquid chromatography (UPLC) was developed [28], which showed some advantages such as reduced run time, increased peak capacities and less solvent consumption, but the technique still requires tedious sample preparation (reflux extraction followed by solid-phase extraction) prior to UPLC analysis. Conventional CE methods were also reported for the analysis of CHE and SAN [16,[29][30][31].…”