Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method

The preparation and characterization of activeloaded microparticles for cosmetic textile applications were studied in this work. The double emulsion diffusion/ evaporation technique was served and the Eudragit® RS100 (ethyl acrylate and methyl methacrylate copolymers) was used as biodegradable polymer. Two actives molecules, vitamin E and Lauryl isoquinolinium bromide (LIB) were successfully loaded during the formulation. Obtained particles were spherical in shape with a little variable diameter about 1 μm and zeta potential of 55 mV. The cutaneous penetration of the particles was evaluated by dermal microdialysis, tape stripping, and punch-biopsy techniques from ex vivo human skin. LIB or vitamin E concentrations in the different layers of the skin were analyzed by high-performance liquid chromatography method. Finally, these particles were adsorbed onto a textile in optimized conditions. The scanning electron microscopy is used to confirm the total threads coverage by particles.

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“…The retention time was 4.8 min and the volume of injection was 20 μl. Linearity was obtained in the range 0.1 to 10 μg/ml (r 2 > 0.99) [18].…”

Section: Sample Analysismentioning

confidence: 90%

Elaboration of sponge-like particles for textile functionalization and skin penetration

et al. 2015

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“…Several different approaches can be used to ascertain LLOD and LLOQ [8,11]. We used the approach that is "based on the standard deviation (S.D.)…”

Section: Llod and Lloq Determinationsmentioning

confidence: 99%

“…1. We have previously reported a highly sensitive LC-MS/MS method to assess the purity and integrity of procaine when accompanied by its hydrolytic products PABA in solid or aqueous solution samples [8]. Wanting to extend this method for general use with various biological matrices, we also appreciated that LC-MS/MS is a relatively expensive technology that is not always readily available in academic research laboratories.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive HPLC assay for simultaneous determination of procaine and para-aminobenzoic acid from human and rat liver tissue extracts

Dhananjeyan

Trendel

Bykowski

et al. 2008

Journal of Chromatography B

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“…The Chinese Pharmacopoeia methods for assaying procaine hydrochloride involve dead-stop titration, the United States Pharmacopoeia detects it with a spectrophotometric method, and electrometric titration is employed by British Pharmacopoeia. In recent years, the reported methods for the determination of procaine hydrochloride can be roughly divided into four categories as follows: titration methods [15,16], electrochemical analysis [17,18], spectrophotometry [19,20] and chromatography [21,22].…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Electrochemical Sensor Based on Solution Polymerized Molecularly Imprinted Polymers for Procaine Detection

et al. 2016

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A sensitive and selective method for the determination of procaine hydrochloride using molecularly imprinted polymers (MIPs) modified glassy carbon electrodes was developed. The MIPs were prepared by solution polymerization using procaine hydrochloride as the template molecules and acrylic acid (AA) and vinyltriethoxysilane (WD‐20) as the functional monomer and cross‐linking agent, respectively. A film was formed on the surface of the glassy carbon electrodes and later cross‐linked with ethanol as solvent. Next, these electrodes were employed to detect procaine hydrochloride by differential pulse voltammetry (DPV). Under the optimized conditions, good linearity (correlation coefficient of 0.9986) was observed between the oxidation peak current and the concentration of procaine hydrochloride in the range of 4.0×10−8 to 2.4×10−5 M in a pH 7.0 0.04 M phosphate buffer solution, and the detection limit (S/N=3) was 1.02×10−8 M. In addition, the stability and reproducibility of the sensors were satisfactory. Moreover, the concentration of procaine hydrochloride in human blood serum samples was detected, and its recoveries ranged from 97.5 % to 106.4 % with RSD less than 2.15 %. These results suggest that the prepared molecularly imprinted electrochemical sensors can be used for the determination of procaine in clinical studies.

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Simultaneous determination of procaine and para-aminobenzoic acid by LC–MS/MS method

Cited by 31 publications

References 34 publications

Elaboration of sponge-like particles for textile functionalization and skin penetration

Elaboration of sponge-like particles for textile functionalization and skin penetration

Rapid and sensitive HPLC assay for simultaneous determination of procaine and para-aminobenzoic acid from human and rat liver tissue extracts

A Sensitive Electrochemical Sensor Based on Solution Polymerized Molecularly Imprinted Polymers for Procaine Detection

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