Simultaneous determination of miconazole and hydrocortisone or mometasone using reversed phase liquid chromatography

Abstract

KEYWORDSTwo simple, rapid and precise reversed phase liquid chromatographic methods have been developed and validated for the simultaneous determination of miconazole nitrate in two binary mixtures, with hydrocortisone acetate (Mixture 1) and mometasone furoate (Mixture 2). For the two mixtures, chromatographic separation was carried out on a C18 column. For mixture 1, a mobile phase consisting of 2.22 mM sodium dihydrogen phosphate (Triethylamine 0.2%):acetonitrile (45:55, v:v) at a flow rate of 0.9 mL/min was used at ambient temperature. Quantitative determination of miconazole and hydrocortisone was achieved with UV detection at 215 and 245 nm, respectively. Linearity, accuracy and precision were found to be acceptable over the concentration range of 30-80 μg/mL for miconazole and 4-80 μg/mL for hydrocotisone. For mixture 2, a mobile phase consisting of acetonitrile:water (Triethylamine 0.2%) (70:30, v:v) at a flow rate of 0.9 mL/min was used at ambient temperature. Quantitative determinations of miconazole and mometasone were achieved with UV detection at 215 and 250 nm, respectively. Linearity, accuracy and precision were found to be acceptable over the concentration range of 10-200 μg/mL for miconazole and 2-60 μg/mL for mometasone. The optimized methods were proved to be specific, robust and accurate for the quality control of the cited drugs in pharmaceutical preparations.

Optimization and Validation of Sensitive UPLC-PDA Method for Simultaneous Determination of Thymoquinone and Glibenclamide in SNEDDs Formulations Using Response Surface Methodology

“…Decreasing the ACN concentrations' sharp peak with narrow width was observed. The same observation was obtained in the separation of miconazole and mometasone, in which the ACN concentration was affected in an antagonistic way by the resolution [40].…”

“…The experimental protocols for simultaneous analysis of RQ and GB were employed with a highly sensitive UPLC system (Ultimate 3000 ® binary solvent manager) that was equipped with an automatic sampler and a Photodiode Array (PDA) detector. The analytical separation of the two compounds was accomplished by reverse-phase isocratic elution by using a mobile phase composed of different ACN concentrations (40,45 and 50% ACN mixed, respectively with 60, 55 and 50% of water containing 0.1% formic acid) and different column temperatures according to the previously mentioned statistical design. The elution flow rate through an Acquity ® UHPLC column HSS C18 (2.1 × 50 mm, 1.7 µm) was 0.3 mL/min.…”

Method Development and Validation for the Simultaneous Analysis of Miconazole Nitrate, Hydrocortisone and Chlorocresol in a Pharmaceutical Topical Cream by Reverse Phase Liquid Chromatography

“…Miconazole/HCA cream is preserved with CHL. It has been revealed that simultaneous methods to separate MCN and HCA from the cream sample matrix were developed in the past [8][9][10][11][12][13][14][15][16], such as reverse phase high-performance liquid chromatography (RP-HPLC), high-performance thin-layer chromatography (HPTLC), derivative UV-Vis spectrophotometry and absorbance ratio spectrophotometry. However, these methods excluded the separation of CHL.…”

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“…[11][12][13][14] Analysis of MZL, along with other drugs such as mometasone furoate, nadifloxacin, lidocaine, econazole, metronidazole, hydrocortisone, and using several analytical techniques has also been reported. [15][16][17][18][19][20][21][22][23][24][25][26] Various authors have developed and reported different HPTLC, HPLC, and UV method, for the estimation of EGL alone, as well as combinations with cinnamon oil, rosmarinic acid, piperine, and cinnamaldehyde. [27][28][29][30][31][32][33][34][35][36][37][38] Attempt was made to develop and validate simpler, sensitive, precise, accurate, and cost-effective UV spectroscopic methods for the simultaneous determination of MZL and EGL in emulgel formulation [Figure 2].…”