Simultaneous determination of glucocorticoids in plasma or urine by high-performance liquid chromatography with precolumn fluorimetric derivatization by 9-anthroyl nitrile

RP-HPLC method with fluorescence detection for determination of small quantities of triamcinolone in plasma in presence of endogenous steroids after derivatization with 9-anthroyl nitrile; pharmacokinetic studies

“…Pharmacokinetic studies after administration of the small, 2-5 mg, formulations of TCA were performed applying a much more sensitive method of RIA coupled with HPLC, where LOQ was 0.1 ng/ml [5,6], but details of the method were not published. The elaborated method with fluorescence detection of TMC derivative was shown to be also adequately sensitive for the 4 mg formulation of TMC, with LOQ equal to 2.5 ng/ml and comparable to the method [8] where six glucocorticoids, but not TMC, were determined. However, a chromatographic column with normal phase was used in the latter method.…”

“…However, no HPLC method is available for determination of TMC. Purification of the received fluorescent esters of glucocorticoids from reagents used for derivatization before injection into the column also presented a very important issue [8]. A very sensitive GC-MS method was also published for determination of TCA in human bronchoalveolar lavage fluid, where analytes were converted to acetate derivatives before GC-MS analysis [9].…”

High‐Performance Liquid Chromatographic Analysis of Psychotropic and Endogenous Compounds

“…Figure 2-1 Fluorescent steroid derivatives formed by the reaction with 9-anthroyl nitrile mixtures. The analysis time for complete separation of these steroids was approximately 100 min (Shibata et al, 1998). Another example using anthroyl nitrile as the derivatizing reagent in the presence of quinuclidine was reported by Kurosawa and associates (1995) on the determination of 18-hydroxycortisol, 18-hydroxycortisone, and 18-oxocortisol.…”

Prednisolone measurement in human serum using liquid chromatography tandem mass spectrometry

“…12 Derivatization is required for GC 10 and some HPLC techniques. 7 Retention times of10 min or more for HPLC 7,8 result in labour-intensive, time-consuming assays. Previously described LC-MS/ MS methods utilize a C18 solid-phase extraction step, again making a more labour-intensive, time-consuming assay.…”