Simultaneous Determination of Cortisol and Corticosterone in Human Plasma by Quantitative Paper Chromatography.

The Metabolism of Corticosterone in Man

“…Bush has reviewed this information (60) and has suggested that the higher plasma corticosterone values previously reported probably resulted from inadequate purification of the steroid prior to analysis. The data on the relative amounts of cortisol to corticosterone in the miscible pool, in the peripheral blood (9)(10)(11)(12)(13)(14)(15)(16), and in urine (13,17) support the concept that corticosterone secretion is very much less than that of cortisol and the turnover rate studies confirm this.5 Other than the fact that corticosterone is metabolized slightly more rapidly than cortisol, the physiological disposition and metabolic fate of these two steroids are not greatly different.…”

“…Although contributing little to the body pool of adrenocortical steroids in man, it is one of the major adrenocortical secretory products in some animals, e.g., rodents (3). In man the major glucocorticosteroid is cortisol and the major mineralocorticosteroid is probably aldosterone; nevertheless, corticosterone has been identified and measured in human adrenal tissue (4), adrenal gland perfusates (5), adrenal vein blood (6)(7)(8), and peripheral blood (9)(10)(11)(12)(13)(14)(15)(16). In all of these tissues the corticosterone concentration was found to be lower than that of cortisol, although the concentration of the biologically important aldosterone has been found to be even less than that of the corticosterone (17,18).…”

Respiratory Gas Analysis

“…T h e follow ing s ta tis tic a l fo rm u la s a n d sym bols are u s e d : [2] *i + x * n = num ber of duplicate analyses d = differences between duplicate analyses d = mean difference between duplicate analyses S j = stand ard deviation of differences betw een duplicates analyses S j = stand ard error of th e mean difference Si = stand ard error of an individual analysis SJ = proportionate stan d ard error of an individual aualysis a = differences betw een duplicate analyses expressed as a percentage of th e m ean of duplicates (proportionate differences) Xj = duplicate analyses x 2 a = proportionate m ean difference betw een duplicate analyses SQ = stand ard deviation of porportionate differences between duplicate analyses S^ = stan d ard error of proportionate mean difference t-test is performed according to FiscnER [5] Tabic I. E rro r on oxygen and carbon dioxide analysis w ith the H aldane and physical analysers. f Difference, probably due to galvanom etric d rift, is significant (P < 0 .0 0 1 ) although sm all, so th a t calculated precision is not absolutely correct.…”

Circadian Rhythm of Plasma 11-Hydroxycorticosteroids in Patients on Chronic Intermittent Dialysis

“…Since, under normal conditions, adrenal cortical activity is at its lowest ebb at night Plasma ll-OH.C.S., the main component of (Migeon et al, 1956;Peterson and Wyngaarden, which is normally cortisol (Bondy and Upton, 1957;Mattingly, 1963) it seems likely that an Peterson, 1957) were measured using the method of abnormality in the secretion of adrenocorticosteroids Mattingly (1962)with the following modifications:-would be reflected by changes in the nocturnal levels Free flowing heparinised blood samples were used of ll-hydroxycorticosteroids (11-0H.C.S. ).…”