Simultaneous determination of biogenic amines and morphine in discrete rat brain regions by high-performance liquid chromatography with electrochemical detection

Simultaneous Measurement of Extracellular Morphine and Serotonin in Brain Tissue and CSF by Microdialysis in Awake Rats

“…We did, however, find a significant increase in the extracellular levels of 5-HIAA and HVA in tissue and in cisternal CSF. These results are consistent with previous data showing that 5-HIAA and HVA levels are increased in tissue (Weil-Fugazza et al, 1979;Godefroy et al, 1980;Kim et al, 1986), in CSF (Bardon and Ruckebusch, 1984), and in extracellular fluid (DiChiara and Imperato, 1988). We did not, however, find an increase in 5-HIAA content in spinal cord, which suggests that monitoring CSF may not necessarily provide f a measure of the changes that occur in tissue.…”

“…It is generally accepted that morphine exerts its analgesic effect, in part, through release of 5-HT at the level of the spinal cord (for reviews, see Fields, 1978, 1984). Consistent with this hypothesis, several studies have reported that morphine increases the turnover of 5-HT in the brain (Weil-Fugazza et al, 1979;Godefroy et al, 1980;Kim et al, 1986) and that an analgesic dose of morphine injected systemically or directly into the brain increases 5-HT levels in spinal cord (Yaksh and Tyce, 1979;Bardon and Ruckebusch, 1984). Most recently, Rivot et al (1988), using in vivo voltammetry, reported an increase in the amplitude of peak "3" (characteristic of 5-HIAA) in the trigeminal nucleus caudalis (the homologue of the spinal cord dorsal horn) of awake rats.…”

Simultaneous Measurement of Extracellular Morphine and Serotonin in Brain Tissue and CSF by Microdialysis in Awake Rats

“…We did, however, find a significant increase in the extracellular levels of 5-HIAA and HVA in tissue and in cisternal CSF. These results are consistent with previous data showing that 5-HIAA and HVA levels are increased in tissue (Weil-Fugazza et al, 1979;Godefroy et al, 1980;Kim et al, 1986), in CSF (Bardon and Ruckebusch, 1984), and in extracellular fluid (DiChiara and Imperato, 1988). We did not, however, find an increase in 5-HIAA content in spinal cord, which suggests that monitoring CSF may not necessarily provide f a measure of the changes that occur in tissue.…”

“…It is generally accepted that morphine exerts its analgesic effect, in part, through release of 5-HT at the level of the spinal cord (for reviews, see Fields, 1978, 1984). Consistent with this hypothesis, several studies have reported that morphine increases the turnover of 5-HT in the brain (Weil-Fugazza et al, 1979;Godefroy et al, 1980;Kim et al, 1986) and that an analgesic dose of morphine injected systemically or directly into the brain increases 5-HT levels in spinal cord (Yaksh and Tyce, 1979;Bardon and Ruckebusch, 1984). Most recently, Rivot et al (1988), using in vivo voltammetry, reported an increase in the amplitude of peak "3" (characteristic of 5-HIAA) in the trigeminal nucleus caudalis (the homologue of the spinal cord dorsal horn) of awake rats.…”

Simultaneous Measurement of Extracellular Morphine and Serotonin in Brain Tissue and CSF by Microdialysis in Awake Rats

“…We did, however, find a significant increase in the extracellular levels of 5-HIAA and HVA in tissue and in cisternal CSF. These results are consistent with previous data showing that 5-HIAA and HVA levels are increased in tissue (Weil-Fugazza et al, 1979;Godefroy et al, 1980;Kim et al, 1986), in CSF (Bardon and Ruckebusch, 1984), and in extracellular fluid (DiChiara and Imperato, 1988). We did not, however, find an increase in 5-HIAA content in spinal cord, which suggests that monitoring CSF may not necessarily provide f a measure of the changes that occur in tissue.…”

“…It is generally accepted that morphine exerts its analgesic effect, in part, through release of 5-HT at the level of the spinal cord (for reviews, see Fields, 1978, 1984). Consistent with this hypothesis, several studies have reported that morphine increases the turnover of 5-HT in the brain (Weil-Fugazza et al, 1979;Godefroy et al, 1980;Kim et al, 1986) and that an analgesic dose of morphine injected systemically or directly into the brain increases 5-HT levels in spinal cord (Yaksh and Tyce, 1979;Bardon and Ruckebusch, 1984). Most recently, Rivot et al (1988), using in vivo voltammetry, reported an increase in the amplitude of peak "3" (characteristic of 5-HIAA) in the trigeminal nucleus caudalis (the homologue of the spinal cord dorsal horn) of awake rats.…”

Simultaneous Measurement of Extracellular Morphine and Serotonin in Brain Tissue and CSF by Microdialysis in Awake Rats

“…We did, however, find a significant increase in the extracellular levels of 5-HIAA and HVA in tissue and in cisternal CSF. These results are consistent with previous data showing that 5-HIAA and HVA levels are increased in tissue (Weil-Fugazza et al, 1979;Godefroy et al, 1980;Kim et al, 1986), in CSF (Bardon and Ruckebusch, 1984), and in extracellular fluid (DiChiara and Imperato, 1988). We did not, however, find an increase in 5-HIAA content in spinal cord, which suggests that monitoring CSF may not necessarily provide f a measure of the changes that occur in tissue.…”

“…It is generally accepted that morphine exerts its analgesic effect, in part, through release of 5-HT at the level of the spinal cord (for reviews, see Fields, 1978, 1984). Consistent with this hypothesis, several studies have reported that morphine increases the turnover of 5-HT in the brain (Weil-Fugazza et al, 1979;Godefroy et al, 1980;Kim et al, 1986) and that an analgesic dose of morphine injected systemically or directly into the brain increases 5-HT levels in spinal cord (Yaksh and Tyce, 1979;Bardon and Ruckebusch, 1984). Most recently, Rivot et al (1988), using in vivo voltammetry, reported an increase in the amplitude of peak "3" (characteristic of 5-HIAA) in the trigeminal nucleus caudalis (the homologue of the spinal cord dorsal horn) of awake rats.…”