Simultaneous determination of 35 ultra‐trace level organophosphorus pesticide residues in Sanjie Zhentong capsules of traditional Chinese medicine using ultra high performance liquid chromatography with tandem mass spectrometry

Abstract

The aim of this study was to develop an analytical method for the simultaneous determination of ultra-trace levels of 35 organophosphorus pesticide residues in Sanjie Zhentong capsules, a traditional Chinese medicine prescription. A method based on multiclass and multiresidue sample preparation was developed. First, samples were hydrated with water at 4°C. A ratio of 1:3 sample/water was used for each of the sample amounts. Then, different extraction solvents were screened. This step was followed by a dispersive solid-phase extraction clean-up procedure using both primary secondary amine and polyamide. A comprehensive sensitive multiresidue liquid chromatography tandem mass spectrometry method was investigated and validated. Good linearity was achieved in the range of 10-500 μg/kg for each analyte. The average recovery ranged from 70 to 120%, except for methamidophos, fonophos, diazinon, and chlorpyriphos-ethyl, the recoveries of which ranged from 60-70% at the lower concentration level. The precision values were lower than 10% for all the compounds in three concentration levels. The limits of detection and limits of quantification values were 0.01-2.1 and 0.05-3.4 μg/kg, respectively. The matrix effects were determined, and most of the compounds showed signal suppression. Finally, this optimized procedure was applied for the analysis of real samples.

A Vortex-Assisted Dispersive Liquid-Liquid Microextraction Followed by UPLC-MS/MS for Simultaneous Determination of Pesticides and Aflatoxins in Herbal Tea

“…It is necessary for using chemical pesticides during the cultivation of Chinese herbal medicines plants. Therefore, pesticides residues are inevitable in Chinese herbal materials [13,14,15]. Ultimately, the pesticide residues maybe also be detected in herbal tea.…”

Monitoring of Organophosphorus Pesticides in Brazilian Food

“…The QuEChERS method changed the mode of multi-residue analysis [41,[47][48][49][50], as it proved suitable for large-scale residue analyzes in a broad variety of matrices [41,44,47], ensuring exact results, accurate and low detection limits for a wide variety of compounds [50,51]. This pretreatment method is very flexible [52,53] and extensively applied in the determination of various pesticide residues in agricultural products [35,46,52,[54][55][56]. There is a need to develop rapid methods for multi-residue determination of the most commonly used pesticides to monitor pesticide residues in food and ensure not only food safety but also compliance with GAP [7,52].…”

“…This pretreatment method is very flexible [52,53] and extensively applied in the determination of various pesticide residues in agricultural products [35,46,52,[54][55][56]. There is a need to develop rapid methods for multi-residue determination of the most commonly used pesticides to monitor pesticide residues in food and ensure not only food safety but also compliance with GAP [7,52].…”