Simultaneous assessment of ethyl carbamate-induced SCEs in murine lymphocytes, bone marrow and alveolar macrophage cells

Goon, Daniel Ter; Conner, Mary K.

doi:10.1093/carcin/5.3.399

Cited by 21 publications

(6 citation statements)

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“…The effect of 2-ME on baseline SCE frequency has been shown in mouse peripheral blood lymphocyte cultures where a 560 /zM concentration of the chemical doubled the baseline SCE frequency observed at lower doses (Goon and Conner, 1984). In addition, reducing agents such as 2-ME modulate mitogeninduced proliferation responses of both lymphoid cell lines and spleen cells (Broome and Jeng, 1973).…”

Section: Introductionmentioning

confidence: 98%

Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes

Aidoo

Morris

et al. 1989

Cell Biol Toxicol

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2-Mercaptoethanol (2-ME) is used as a medium supplement to enhance the proliferation of lymphocytes cultured in vitro. In this study, we have examined the effects of 2-ME on cell growth and on SCE induction in cultures of unstimulated and phytohemagglutinin (PHA)-stimulated Fischer 344 rat lymphocytes. There were virtually no metaphases detected in cells cultured without PHA. In PHA-stimulated cultures, 2-ME decreased SCE frequency but it enhanced SCE frequency in the presence of 5 to 12.5 microM bromodeoxyuridine (BRdU). Both mitotic and replication indices were increased in the PHA/2-ME system. The levels of incorporated exogenous thymidine, in the presence of 2-ME, were relatively low in unstimulated cells, suggesting that 2-ME is not mitogenic for T-cells. However, 2-ME enhanced PHA-induced response of T-cells as evidenced by increased levels of thymidine incorporation into cellular DNA. The growth-promoting effects and the decrease in SCE frequency caused by 2-ME upon PHA stimulation indicate that 2-ME may alter the nature of interaction between PHA and cellular activating properties or the replicative processes.

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Section: Introductionmentioning

confidence: 98%

Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes

Aidoo

Morris

et al. 1989

Cell Biol Toxicol

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“…Goon and Conner [1984] state that 2-ME is required to obtain successful growth of mouse IL PBLs, although MI data were not presented. However, Speit et al [1980] found that 10 pM 2-ME increased the frequency of CAs and 50 pM induced SCEs in V-79 cells.…”

Section: Resultsmentioning

confidence: 99%

“…In the few previous reports by other investigators culturing mouse IL PBLs for cytogenetic endpoints [Barren et al, 1982;Goon and Conner, 19841 2-ME is used as a growth supplement with some success. Goon and Conner [1984] state that 2-ME is required to obtain successful growth of mouse IL PBLs, although MI data were not presented. However, Speit et al [1980] found that 10 pM 2-ME increased the frequency of CAs and 50 pM induced SCEs in V-79 cells.…”

Section: Resultsmentioning

confidence: 99%

A modified mouse peripheral blood lymphocyte culture system for cytogenetic analysis

Erexson

Kligerman

1987

Environ and Mol Mutagen

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A detailed methodology is presented for culturing mouse peripheral blood lymphocytes isolated on density gradients and stimulated to divide using either phytohemagglutinin, concanavalin A, or lipopolysaccharide. The techniques described yield more than sufficient numbers of mitotic cells for analyzing sister chromatid exchange, chromosome aberrations, and micronuclei following in vitro or in vivo exposure to chemicals or radiation.

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“…4 ppm. An increase in the percentage of first-division metaphases, as well as decreases in the percentages of second and third or higher division metaphases, were observed in the low dose exposure group.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of sister chromatid exchange and cytotoxicity in murine tissues in vivo and lymphocytes in vitro following methyl isocyanate exposure.

Conner¹,

Rubinson²,

Ferguson³

et al. 1987

Environ Health Perspect

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The purpose of this study was to assess sister chromatid exchange (SCE) levels and cell cycle kinetics in various murine tissues following MIC exposure. Following exposure of mice to MIC, these parameters were measured in bone marrow and alveolar macrophages labeled with BrdUrd in vivo and in peripheral blood and spleen lymphocytes cultured in the presence of BrdUrd in vitro. Target concentrations of MIC were 2, 15, and 30 ppm (3 hr).Neither elevated SCE frequencies nor inhibition of cell cycling were evident in lipopolysaccharide (LPS)-or concanavalin A (ConA)-stimulated spleen lymphocytes, or in LPS-stimulated peripheral blood lymphocyte (PBL) cultures from mice exposed for 3 hr to MIC concentrations as high as 30.5 ppm. Inhibition of cell cycling and poor culture success rates were apparent in ConA-stimulated PBLs following MIC exposures as low as 2.3 ± 0.4 ppm for 3 hr.At the lowest MIC dose employed, the cycling characteristics of bone marrow and alveolar macrophages were not altered, and SCE frequencies were at control levels. However, severe cell cycle inhibition was observed in these tissues at MIC concentrations of 15 ppm or greater. A marker of cytotoxicity at this dose was a high frequency (approximately 33-90%) of occurrence of first division cells containing a latereplicating Y chromosome.Despite its apparent cellular toxicity, MIC is not genotoxic as measured by SCE analysis in the tissues examined in this study.

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Simultaneous assessment of ethyl carbamate-induced SCEs in murine lymphocytes, bone marrow and alveolar macrophage cells

Cited by 21 publications

References 0 publications

Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes

Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes

A modified mouse peripheral blood lymphocyte culture system for cytogenetic analysis

Evaluation of sister chromatid exchange and cytotoxicity in murine tissues in vivo and lymphocytes in vitro following methyl isocyanate exposure.

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