Simultaneous analysis of retinol, six carotenoids, two tocopherols, and coenzyme Q10 from human plasma by HPLC

Boulet, Lysiane; Alex, Brigitte; Clavey, Nadège; Martinez, Julie; Ducros, Véronique

doi:10.1016/j.jchromb.2020.122158

Cited by 11 publications

(5 citation statements)

References 27 publications

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“…The retinoid analytic methods described in the literature include (ultra) high-performance liquid chromatography ((U)HPLC) coupled with spectrophotometric (UV-Vis/DAD), spectrofluorimetric (FLD), electrochemical (ECD) detection, mass spectrometry (MS and MS/MS), and also supercritical fluid chromatography (SFC) and immunoassay-based ones, featuring different sensitivities, effectiveness towards different biological matrices, benefits, and limitations. A comparison of these methodologies is provided in Table 2 [ 279 , 280 , 281 , 282 , 283 , 284 , 285 , 286 , 287 , 288 , 289 , 290 , 291 , 292 , 293 , 294 , 295 , 296 , 297 , 298 , 299 , 300 , 301 , 302 , 303 , 304 , 305 , 306 , 307 , 308 , 309 , 310 , 311 , 312 , 313 , 314 , 315 , 316 , 317 , 318 , 319 , 320 , 321 , 322 , 323 , 324 ].…”

Section: Analytical Approaches For Measuring Vitamin a Levelsmentioning

confidence: 99%

“…Whereas single wavelength and DAD are effective for in vitro retinoid assays and quantitation of abundant endogenous retinoids (retinal, retinol, retinoid esters) in vivo, they usually lack the needed sensitivity for making an endogenous bioanalysis of retinoid acid. In fact, concentrations of endogenous ATRA in tissues are up to several orders of magnitude below the limit of detection and/or limit of quantification for both DAD and single-wavelength detection [ 279 , 280 , 281 , 282 , 283 , 284 , 285 , 286 , 287 , 288 , 289 , 290 , 291 , 292 , 293 , 294 , 295 , 320 , 321 , 322 , 323 , 324 ].…”

Section: Analytical Approaches For Measuring Vitamin a Levelsmentioning

confidence: 99%

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Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity

et al. 2021

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Vitamin A is a group of vital micronutrients widely present in the human diet. Animal-based products are a rich source of the retinyl ester form of the vitamin, while vegetables and fruits contain carotenoids, most of which are provitamin A. Vitamin A plays a key role in the correct functioning of multiple physiological functions. The human organism can metabolize natural forms of vitamin A and provitamin A into biologically active forms (retinol, retinal, retinoic acid), which interact with multiple molecular targets, including nuclear receptors, opsin in the retina and, according to the latest research, also some enzymes. In this review, we aim to provide a complex view on the present knowledge about vitamin A ranging from its sources through its physiological functions to consequences of its deficiency and metabolic fate up to possible pharmacological administration and potential toxicity. Current analytical methods used for its detection in real samples are included as well.

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Section: Analytical Approaches For Measuring Vitamin a Levelsmentioning

confidence: 99%

Section: Analytical Approaches For Measuring Vitamin a Levelsmentioning

confidence: 99%

Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity

et al. 2021

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“… 2019 ; Boulet et al. 2020 ), and its gradient elution was fine‑tuned when other factors, such as sample pre-treatment procedures and IS, were optimized. Several carotenoids, such as β‑carotene, β‑cryptoxanthin, lutein, and lycopene, were screened for their potential use as IS.…”

Section: Resultsmentioning

confidence: 99%

Factors determining the oral absorption and systemic disposition of zeaxanthin in rats: in vitro , in situ , and in vivo evaluations

Seo

Han

Choi

et al. 2022

Pharmaceutical Biology

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Context Zeaxanthin is a yellow‑coloured dietary carotenoid widely recognized as an essential component of the macula. It exerts blue light filtering and antioxidant activities, offering eye health and vision benefits. Objective This study explores the oral absorption and systemic disposition of zeaxanthin from biopharmaceutical and pharmacokinetic perspectives. Materials and methods In vivo intravenous (5 and 10 mg/kg) and intraportal (5 mg/kg) pharmacokinetic studies were performed to determine intrinsic tissue‑blood partition coefficient, elimination pathway, and hepatic clearance, of zeaxanthin in rats. Moreover, in vitro physicochemical property test, in situ closed loop study, in vivo oral pharmacokinetic study (20 and 100 mg/kg), and in vivo lymphatic absorption study (100 mg/kg) were conducted to investigate the gut absorption properties of zeaxanthin and assess the effects of several lipids on the lymphatic absorption of zeaxanthin in rats. Results Zeaxanthin exhibited poor solubility (≤144 ng/mL) and stability (6.0–76.9% of the initial amount remained at 24 h) in simulated gut luminal fluids. Gut absorption of zeaxanthin occurred primarily in the duodenum, but the major fraction (≥84.7%) of the dose remained unabsorbed across the entire gut tract. Considerable fractions of intravenous zeaxanthin accumulated in the liver, lung, and spleen (21.3, 11.7, and 2.0%, respectively). It was found that the liver is the major eliminating organ of zeaxanthin, accounting for 53.5–90.1% of the total clearance process (hepatic extraction ratio of 0.623). Discussion and conclusions To our knowledge, this is the first systematic study to report factors that determine the oral bioavailability and systemic clearance of zeaxanthin.

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“…Luteína, zeaxantina y licopeno son carotenoides que no son provitamina A pero son bien conocidos por tener un papel protector contra los procesos oxidativos y son de interés en enfermedades crónicas (Boulet et al, 2020). Además, la ingesta de carotenoides se asocia con una menor incidencia de enfermedades cardiovasculares debido a sus mecanismos como el captador de radicales libres, y la resistencia del colesterol de lipoproteínas de baja densidad a los inductores de oxidación (Panaite et al, 2021).…”

Section: Efecto Cardiovascularunclassified

“…A pesar de su gran diversidad en la naturaleza, pocos carotenoides se almacenan en los tejidos y fluidos animales, lo que sugiere que se acumulan selectivamente o se transforman metabólicamente para su almacenamiento (Babin et al, 2020). Las concentraciones plasmáticas más altas de carotenoides se han relacionado con un riesgo reducido de incidencia de cáncer y enfermedades cardiovasculares en seres humanos (Boulet et al, 2020). Los niveles reducidos de carotenoides en plasma sanguíneo se han relacionado con una variedad de estados de enfermedad, incluida la infección por VIH, malaria, riesgo de algunos tipos de cáncer e infarto de miocardio (Perrett et al, 2020).…”

Section: Efectos Adversos De Los Carotenoidesunclassified

Carotenoides

Romero,

Espinoza

2024

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Con gran satisfacción y entusiasmo se presenta ante la comunidad científica, la presente obra, un libro dedicado a despejar las innumerables dudas respecto al conocimiento de las características, propiedades y métodos de extracción de carotenoides: carotenos y xantófilas. Este libro enfatiza en la importancia que tiene conocer acerca de la estructura, propiedades, biosíntesis, biodisponibilidad en el cuerpo humano, factores que afectan su biodisponibilidad y técnicas de extracción de los carotenoides es para conocer el fundamento de sus funciones esenciales tanto en las plantas como en el ser vivo, en plantas, captan la energía luminosa y actúan como antioxidantes. En el ser vivo al consumir los carotenoides se transforman en vitamina A, micronutriente que interviene en una amplia variedad de funciones fisiológicas, entre las que destaca la función visual, formación y mantenimiento de tejidos blandos, dientes, mucosas y piel, y tiene también un papel clave en el desarrollo fetal, embarazo y lactancia.

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Simultaneous analysis of retinol, six carotenoids, two tocopherols, and coenzyme Q10 from human plasma by HPLC

Cited by 11 publications

References 27 publications

Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity

Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity

Factors determining the oral absorption and systemic disposition of zeaxanthin in rats: in vitro , in situ , and in vivo evaluations

Carotenoides

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