Simultaneous achiral-chiral analysis of pharmaceutical compounds using two-dimensional reversed phase liquid chromatography-supercritical fluid chromatography

Recent Advances in Chromatography for Pharmaceutical Analysis

“…26 In addition to RPLC, SFC is also a potentially attractive technique as a fast second dimension ( Figure 4). In this context, several 2D-LCxSFC applications have recently been proposed for simultaneous achiral-chiral analysis, 27 25 It is clear that the greatest challenge in 2D-LC method development is to overcome incompatibility between the solvent systems, namely, the influence that the effluent of the first dimension can have on the performance of the second dimension. However, Stoll et al 29 paved the way to new possibilities and applications by proposing a valve-based approach, referred to as Active Solvent Modulation (ASM), specifically designed to address this incompatibility issue.…”

Supercritical Fluid Chromatography and Its Application in Lipid Isomer Separation

“…Through coupling with other separation methods, high-efficient and high-throughput 2D systems of SFC 9 GC [16] or RPLC 9 SFC [17], and even SFC 9 SFC [18] can be achieved. The most common detection method combined with SFC is MS. SFC-MS offers the advantages of high separation efficiency and detection sensitivity.…”

Fraction transfer process in on‐line comprehensive two‐dimensional liquid‐phase separations

“…The “classical” eight‐port valve with the 100 μL loops has recently been applied for LC × LC separation of polymers . Moreover, the four‐port duo valves have been employed in the analyses of synthetic dyes , surfactants , polymers , natural antioxidants , pharmaceutically important compounds , peptides , glycosides , and digests of monoclonal antibodies . The combination of the four‐port duo valve with two eight‐column selector valves equipped with 40 μL sampling loops has been used for the analysis of biopharmaceuticals as reported by Stoll et al.…”