Simulated spatial radiation impacts learning and memory ability with alterations of neuromorphology and gut microbiota in mice

Song, Chen; Gao, Xin; Song, Wei; Zeng, Deyong; Shan, Shan; Yin, Yishu; Li, Yongzhi; Baranenko, Denis; Lu, Weihong

doi:10.1039/d0ra01017k

Cited by 8 publications

(6 citation statements)

References 67 publications

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“…The brain is highly sensitive to radiation, which usually causes a decline in learning and memory 28,29 . Our previous study also confirmed this conclusion 30 . AS is a kind of wild herb with the homology of medicine and food.…”

Section: Discussionsupporting

confidence: 77%

Acanthopanax senticosus extract alleviates radiation‐induced learning and memory impairment based on neurotransmitter‐gut microbiota communication

et al. 2023

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Background Acanthopanax senticosus (AS) is a medicinal and food plant with many physiological functions, especially nerve protection. Its extract has many functional components, including polysaccharides, flavonoids, saponins, and amino acids. Our previous study indicated that AS extract protected against nerve damage caused by radiation. However, little is known about the gut‐brain axis mechanism of AS and its impact on radiation‐induced learning and memory impairment. Method In 60Co‐γ ray‐irradiated mice, we investigated the changes in behavior, neurotransmitters and gut microbiota after different days of administration of AS extract as a dietary supplement. Results The AS extract improved learning and memory ability in mice, and the neurotransmitter levels in the hippocampus and colon started to change from the 7th day, which accompanied changes of the gut microbiota, a decreased abundance of Helicobacter on the 7th day and an increased abundance of Lactobacillus on the 28th day. Among the marker bacteria, Ruminococcus and Clostridiales were associated with 5‐HT synthesis, and Streptococcus were associated with 5‐HT and ACH synthesis. In addition, the AS extract increased the tight junction protein, inhibited inflammation levels in colon, and even increased the relative protein expression of BDNF and NF‐κB and decreased the relative protein expression of IκBα in the hippocampus of irradiated mice. Conclusion These results will lay the foundation for further study on the mechanism of the gut‐brain axis of AS in preventing radiation‐induced learning and memory impairment.

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Section: Discussionsupporting

confidence: 77%

Acanthopanax senticosus extract alleviates radiation‐induced learning and memory impairment based on neurotransmitter‐gut microbiota communication

et al. 2023

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“…The hippocampus consists of anatomically different but functionally related subdomains containing different cell types (Xu et al, 2022; Zhao et al, 2008). The dentate gyrus (DG) is one of two brain regions where neurogenesis occurs throughout life (Zhao et al, 2008), and the DG is particularly sensitive to radiation (Mizumatsu et al, 2003; Monje, 2008; Schultz & Rolls, 1999; Song et al, 2020). However, compared with those in other brain regions, the neurons within the CA1 region are the most sensitive to neurodegeneration associated with Alzheimer's disease or ischemic episodes (Mizumatsu et al, 2003; Monje, 2008; Schultz & Rolls, 1999; Soltesz & Losonczy, 2018, Song et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The dentate gyrus (DG) is one of two brain regions where neurogenesis occurs throughout life (Zhao et al, 2008), and the DG is particularly sensitive to radiation (Mizumatsu et al, 2003; Monje, 2008; Schultz & Rolls, 1999; Song et al, 2020). However, compared with those in other brain regions, the neurons within the CA1 region are the most sensitive to neurodegeneration associated with Alzheimer's disease or ischemic episodes (Mizumatsu et al, 2003; Monje, 2008; Schultz & Rolls, 1999; Soltesz & Losonczy, 2018, Song et al, 2020). Currently, there are no systematic studies about the molecular changes within the hippocampus after irradiation.…”

Section: Discussionmentioning

confidence: 99%

CACNA1B protects naked mole‐rat hippocampal neuron from apoptosis via altering the subcellular localization of Nrf2 after ⁶⁰Co irradiation

Yang,

Chen,

Jiang

et al. 2024

Cell Biology International

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Although radiotherapy is the most effective treatment modality for brain tumors, it always injures the central nervous system, leading to potential sequelae such as cognitive dysfunction. Radiation induces molecular, cellular, and functional changes in neuronal and glial cells. The hippocampus plays a critical role in learning and memory; therefore, concerns about radiation‐induced injury are widespread. Multiple studies have focused on this complex problem, but the results have not been fully elucidated. Naked mole rat brains were irradiated with 60Co at a dose of 10 Gy. On 7 days, 14 days, and 28 days after irradiation, hippocampi in the control groups were obtained for next‐generation sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were subsequently performed. Venn diagrams revealed 580 differentially expressed genes (DEGs) that were common at different times after irradiation. GO and KEGG analyses revealed that the 580 common DEGs were enriched in molecular transducer activity. In particular, CACNA1B mediated regulatory effects after irradiation. CACNA1B expression increased significantly after irradiation. Downregulation of CACNA1B led to a reduction in apoptosis and reactive oxygen species levels in hippocampal neurons. This was due to the interaction between CACNA1B and Nrf2, which disturbed the normal nuclear localization of Nrf2. In addition, CACNA1B downregulation led to a decrease in the cognitive functions of naked mole rats. These findings reveal the pivotal role of CACNA1B in regulating radiation‐induced brain injury and will lead to the development of a novel strategy to prevent brain injury after irradiation.

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“…Water maze was used to evaluate the learning and memory ability according to our previous method 46 . Briefly, as shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Eleutheroside E supplementation prevents radiation-induced cognitive impairment and activates PKA signaling via gut microbiota

Song

Duan

et al. 2022

Commun Biol

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Radiation affects not only cognitive function but also gut microbiota. Eleutheroside E (EE), a principal active compound of Acanthopanax senticosus, has a certain protective effect on the nervous system. Here, we find a four-week EE supplementation to the 60Co-γ ray irradiated mice improves the cognition and spatial memory impairments along with the protection of hippocampal neurons, remodels the gut microbiota, especially changes of Lactobacillus and Helicobacter, and altered the microbial metabolites including neurotransmitters (GABA, NE, ACH, 5-HT) as well as their precursors. Furthermore, the fecal transplantation of EE donors verifies that EE alleviated cognition and spatial memory impairments, and activates the PKA/CREB/BDNF signaling via gut microbiota. Our findings provide insight into the mechanism of EE effect on the gut-brain axis and underpin a proposed therapeutic value of EE in cognitive and memory impairments induced by radiation.

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Simulated spatial radiation impacts learning and memory ability with alterations of neuromorphology and gut microbiota in mice

Abstract: Exposure to 60Co-γ ray impacts learning and memory ability as well as the cell morphology and neurotransmitters in hippocampus, even disrupts the bacterial community in colon.

Cited by 8 publications

References 67 publications

Acanthopanax senticosus extract alleviates radiation‐induced learning and memory impairment based on neurotransmitter‐gut microbiota communication

Acanthopanax senticosus extract alleviates radiation‐induced learning and memory impairment based on neurotransmitter‐gut microbiota communication

CACNA1B protects naked mole‐rat hippocampal neuron from apoptosis via altering the subcellular localization of Nrf2 after ⁶⁰Co irradiation

Eleutheroside E supplementation prevents radiation-induced cognitive impairment and activates PKA signaling via gut microbiota

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Simulated spatial radiation impacts learning and memory ability with alterations of neuromorphology and gut microbiota in mice

Abstract: Exposure to 60Co-γ ray impacts learning and memory ability as well as the cell morphology and neurotransmitters in hippocampus, even disrupts the bacterial community in colon.

Cited by 8 publications

References 67 publications

Acanthopanax senticosus extract alleviates radiation‐induced learning and memory impairment based on neurotransmitter‐gut microbiota communication

Acanthopanax senticosus extract alleviates radiation‐induced learning and memory impairment based on neurotransmitter‐gut microbiota communication

CACNA1B protects naked mole‐rat hippocampal neuron from apoptosis via altering the subcellular localization of Nrf2 after 60Co irradiation

Eleutheroside E supplementation prevents radiation-induced cognitive impairment and activates PKA signaling via gut microbiota

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CACNA1B protects naked mole‐rat hippocampal neuron from apoptosis via altering the subcellular localization of Nrf2 after ⁶⁰Co irradiation