Simulated Digestion and Fermentation In Vitro by Obese Human Gut Microbiota of Sulforaphane from Broccoli Seeds

Sun, Yifei; Tang, Zhaocheng; Hao, Tingting; Qiu, Zhiren; Zhang, Baolong

doi:10.3390/foods11244016

Cited by 9 publications

(5 citation statements)

References 59 publications

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“…A previous study had shown that Phellinus linteus polysaccharide could effectively inhibit the relative abundance of Escherichia–Shigella , a kind of pathogenic bacteria. In the groups incubated with sulforaphane from broccoli seeds, the relative abundance of harmful bacterium genera, Clostridium_sensu_stricto_1, decreased . Similarly, HSPs could decrease the abundance of these harmful bacterial communities, which might be the key genera for HSP regulation.…”

Section: Resultsmentioning

confidence: 98%

The Regulatory Effect of Huangshui Polysaccharides on Intestinal Microbiota and Metabolites during In Vitro Fermentation

Li,

Su,

et al. 2024

J. Agric. Food Chem.

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Huangshui polysaccharides (HSPs) have attracted extensive attention recently for their biological activity and physicochemical property. This research investigated the extraction, structural characterization, and prebiotic activity of three different HSPs (HSP40-0, HSP60-0, and HSP80-0) in vitro to reveal the scientific support for the high-value utilization of Huangshui. HSPs were heteropolysaccharide with diverse structures and surface morphologies. Comprehensive analysis was conducted through 16S rRNA gene sequencing and metabolite profiling techniques, and results showed that HSPs had different potentials to regulate the gut microbiota due to their different structures; for instance, both HSP40-0 and HSP80-0 could notably increase the relative abundance of Bacteroidota, whereas HSP60-0 could increase the relative abundance of Phascolarctobacterium. In addition, HSPs upregulated beneficial differential metabolites, especially short-chain fatty acids (SCFAs). Fermentation products containing these metabolites exhibited anti-inflammatory effects on LPS-treated Caco-2 cells. This study will provide reference for exploring the relationship between the natural polysaccharide structure and the prebiotic activity and widen the application of Huangshui.

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Section: Resultsmentioning

confidence: 98%

The Regulatory Effect of Huangshui Polysaccharides on Intestinal Microbiota and Metabolites during In Vitro Fermentation

Li,

Su,

et al. 2024

J. Agric. Food Chem.

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“…The exploration of natural compounds and their analogs as chemopreventive agents is gaining momentum. SFN, known for its health benefits, is garnering attention in the medical field, especially for its potential protective effects against various types of cancer [38][39][40][41][42][43], cardiovascular diseases [44][45][46], neurological diseases [47][48][49][50], insulin resistance [51][52][53], obesity [54][55][56], and musculoskeletal diseases [57][58][59]. Additionally, the consumption of broccoli, brussels sprouts, cabbage, and SFN, an isothiocyanate derivative, is linked to various health benefits, including anticancer and antioxidant properties [60].…”

Section: Discussionmentioning

confidence: 99%

Sulforaphane Inhibits IL-1β-Induced IL-6 by Suppressing ROS Production, AP-1, and STAT3 in Colorectal Cancer HT-29 Cells

Sah,

Arjunan,

Park

et al. 2024

Antioxidants

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Colorectal cancer (CRC) stands as a major cause of cancer-related mortality globally, accounting for approximately 881,000 deaths each year. Traditional approaches such as chemotherapy and surgery have been the primary treatment modalities, yet the outcomes for patients with metastatic CRC are often unsatisfactory. Recent research has focused on targeting the pathways involved in oxidative stress, inflammation, and metastasis to enhance the survival of CRC patients. Within this context, sulforaphane (SFN), a notable phytochemical found predominantly in cruciferous vegetables, has been recognized as a potential anticancer agent. However, the specific mechanisms through which SFN may exert its chemopreventive effects in CRC remain unclear. This study explores the impact of SFN on IL-1β-induced IL-6 activation and MAPK and AP-1 signaling in HT-29 cells. Our findings reveal that SFN treatment not only diminishes IL-1β-stimulated IL-6 expression but also reduces oxidative stress by curtailing reactive oxygen species (ROS) production. Furthermore, it hinders the proliferation and invasiveness of HT-29 cells through the modulation of MAPK/AP-1 and STAT3 signaling pathways. These results indicate that SFN mitigates IL-1β-induced IL-6 expression in CRC cells by attenuating ROS production and disrupting MAPK/AP-1 signaling. This suggests that SFN holds significant potential as a chemotherapeutic agent for both treating and preventing CRC.

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“…According to a previously described method Solaesa et al, 2022;Sun et al, 2022), in vitro fermentation was conducted using undigested rice flour. On the day of the fermentation experiment, fresh fecal samples were collected from three obese volunteers aged between 24 and 32 years, with a body mass index of 25-35 kg/m 2 .…”

Section: In Vitro Fermentationmentioning

confidence: 99%

“…The bacterial cell density (OD600) of each type of rice flour after in vitro fermentation as measured at 600 nm using the Spark Multimode Plate Reader (Tecan, Männedorf, Switzerland). The content of SCFAs was determined by gas chromatography as previously described (Chen et al, 2017;Sun et al, 2022). In brief, 1.0 mL of sample was combined with 50 μL of phosphoric acid (34%), and the SCFA content was analyzed using the Shimadzu GC-2014 system (Kyoto, Japan) equipped with a flame ionization detector (FID) and a DB-FFAP column (30 m × 0.25 mm × 0.25 μm).…”

Section: Short-chain Fatty Acids Determinationmentioning

confidence: 99%

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High-resistant starch and low-glutelin content 1 rice benefits gut function in obese patients

Zhou,

Tang,

Liu

et al. 2024

Front. Sustain. Food Syst.

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The intestinal microbiota plays a crucial role in human health, yet the impact of high-resistant starch and low-glutelin rice in the gut of obese individuals remains unexplored. In this study, different types of rice, namely japonica rice (control), low-glutelin content 1 rice (Lgc1), high-resistant starch and low-glutelin content 1 rice (HR + Lgc1), and commercially available low-glycemic index rice (LowGI), were utilized as samples to examine their in vitro digestion and fermentation, as well as the impact of HR + Lgc1 on the intestinal microbiota in obese individuals. The findings revealed that HR + Lgc1 rice exhibited lower in vitro digestion rates for starch and protein compared to the other three types. Following 24 h of in vitro fermentation, the total short-chain fatty acid content in HR + Lgc1 was 108.67 mmol/L, which was 60.33, 51.26, and 17.29% that in control, Lgc1, and LowGI, respectively. Moreover, HR + Lgc1 rice displayed an inhibitory effect on the production of harmful branched-chain fatty acid metabolites. Analysis through 16S rDNA sequencing indicated that, after fermentation, HR + Lgc1 significantly enhanced the abundance of beneficial bacteria such as Bifidobacterium, Parabacteroides, and Faecalibacterium in the gut, whereas it reduced the abundance of harmful bacteria such as Escherichia-shigella. Our findings may contribute to the development of new foods rich in dietary fiber to improve the digestive function of patients with multiple comorbidities.

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Simulated Digestion and Fermentation In Vitro by Obese Human Gut Microbiota of Sulforaphane from Broccoli Seeds

Cited by 9 publications

References 59 publications

The Regulatory Effect of Huangshui Polysaccharides on Intestinal Microbiota and Metabolites during In Vitro Fermentation

The Regulatory Effect of Huangshui Polysaccharides on Intestinal Microbiota and Metabolites during In Vitro Fermentation

Sulforaphane Inhibits IL-1β-Induced IL-6 by Suppressing ROS Production, AP-1, and STAT3 in Colorectal Cancer HT-29 Cells

High-resistant starch and low-glutelin content 1 rice benefits gut function in obese patients

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