Simplified Acid Orcein and Giemsa Technique for Routine Staining of Skin Sections

Pinkus, Hermann; Hunter, Rosie

doi:10.1001/archderm.1960.01580050041004

Cited by 31 publications

(13 citation statements)

References 1 publication

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“…Blood vessels in dental pulps of freshly isolated molars were analyzed using Orcein/Giemsa Staining [64] and immunohistochemistry with CD31 antibody for detection of endothelial cells in blood vessels [65]. Mandibles obtained from P5-7 and P18-21 mice were fixed, decalcified, and embedded in paraffin using standard protocols.…”

Section: Methodsmentioning

confidence: 99%

Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars

Balic

Aguila

Caimano

et al. 2010

Bone

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In the past few years there have been significant advances in the identification of putative stem cells also referred to as "mesenchymal stem cells" (MSC) in dental tissues including the dental pulp. It is thought that MSC in dental pulp share certain similarities with MSC isolated from other tissues. However, cells in dental pulp are still poorly characterized. This study focused on the characterization of progenitor and stem cells in dental pulps of erupted and unerupted mice molars. Our study showed that dental pulps from unerupted molars contain a significant number of cells expressing CD90+/ CD45-, CD117+/CD45-, Sca-1+/CD45-and little if any CD45+ cells. Our in vitro functional studies showed that dental pulp cells from unerupted molars displayed extensive osteo-dentinogenic potential but were unable to differentiate into chondrocytes and adipocytes. Dental pulp from erupted molars displayed a reduced number of cells, contained higher percentage of CD45+ and lower percentage of cells expressing CD90+/CD45-, CD117+/CD45-as compared to unerupted molars. In vitro functional assays demonstrated the ability of a small fraction of cells to differentiate into odontoblasts, osteoblasts, adipocytes and chondrocytes. There was a significant reduction in the osteo-dentinogenic potential of the pulp cells derived from erupted molars compared to unerupted molars. Furthermore, the adipogenic and chondrogenic differentiation of pulp cells from erupted molars was dependent on a long induction period and infrequent. Based on these findings we propose that the dental pulp of the erupted molars contain a small population of multipotent cells, whereas the dental pulp of the unerupted molars does not contain multipotent cells but is enriched in osteodentinogenic progenitors engaged in the formation of coronal and radicular odontoblasts.

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Section: Methodsmentioning

confidence: 99%

Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars

Balic

Aguila

Caimano

et al. 2010

Bone

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“…Each biopsy specimen was studied with the dopa reaction [9], periodic acidSchiff reaction (PAS), acid orcein-Giemsa stain (10] and the hematoxylin-eosin stain.…”

Section: Methodsmentioning

confidence: 99%

Cellular Response of Seborrheic Keratosis Following Croton Oil Irritation and Surgical Trauma

Mevorah¹,

Mishima²

1965

Dermatology

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“…A study of typical vitíligo vulgaris in a 38-year-old Caucasian female (Y-177) correspondingly revealed that these granules are disc¬ like bodies basically similar to those of Langerhans cells, averaging up to 250 to 400 m/t X 25 to 40 m/i in size, and exhibit parallel internal striations (Fig 8). 7. Albinism.-Our study on four typical albino subjects included two Negro females (Y-169, Y-234), and a Caucasian and Negro male (E-149, Y-184), ranging in age from 7 to 56 years.…”

Section: Electron Microscopymentioning

confidence: 99%

Macromolecular Changes in Pigmentary Disorders

Mishima¹

1965

Arch Dermatol

173

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Simplified Acid Orcein and Giemsa Technique for Routine Staining of Skin Sections

Cited by 31 publications

References 1 publication

Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars

Characterization of stem and progenitor cells in the dental pulp of erupted and unerupted murine molars

Cellular Response of Seborrheic Keratosis Following Croton Oil Irritation and Surgical Trauma

Macromolecular Changes in Pigmentary Disorders

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