Simple Method for Simultaneous Determination of Halothane, Enflurane, and Isoflurane in Krebs Solution Using Capillary Gas Chromatography

Abstract

A simple gas chromatography (GC) method for the simultaneous determination of halothane, enflurane, and isoflurane in Krebs buffer solution has been developed. The method utilizes methylene chloride as the internal standard and liquid–liquid extraction using chloroform as the solvent. The method demonstrated excellent recovery (100%) of each component and a linear calibration range of 100–700, 100–800, and 300–1400 μg/mL for halothane, isoflurane, and enflurane, respectively. Intra‐day accuracy and precision had an error and coefficient of variation of less than 5.1% and 2.7%, respectively. © 1997 by John Wiley & Sons, Ltd.

Liquid chromatographic determination including simultaneous “on-cartridge” separation of ranitidine cisapride drug combinations from paediatric plasma samples using an automated solid-phase extraction procedure

“…Although HPLC methods for the determination of plasma ranitidine concentrations vary widely, isocratic reverse-phase HPLC using a -Bondpack C18 packed analytical column appears to be the most common mode selected [3,4,9,10,12,13]. Chromatographic separation of ranitidine from plasma constituents has been achieved using a wide variety of mobile phase conditions, however, in more recent years a phosphate-buffered acetonitrile mobile phase has frequently been chosen [3,[7][8][9]12,14]. Despite such a wide variety of HPLC methods it is only recently that a method suitable for the analysis a paediatric plasma samples has been reported [15].…”

“…The analysis of ranitidine in human plasma is frequently performed using HPLC [3][4][5][6][7][8][9][10][11][12][13][14]. Although HPLC methods for the determination of plasma ranitidine concentrations vary widely, isocratic reverse-phase HPLC using a -Bondpack C18 packed analytical column appears to be the most common mode selected [3,4,9,10,12,13].…”