Simple media and conditions for inter-laboratory transport ofCampylobacter jejuniisolates

Abstract

Background: Campylobacter jejuni is one of the most important agents of zoonotic disease. Production as well as companion animals can be the infectious source for Campylobacteriosis in humans. Hence, epidemiological research on animal colonization, survival in food of animal origin, and human Campylobacteriosis is of high priority. As such studies involve worldwide co-operations and should include further typing of isolates in reference centers, using a reliable method for transportation is essential. In the case of C. jejuni, a pathogenic and microaerophilic bacterium, special safety precautions as well as particular transport conditions that guarantee survival of isolates are required. Objective: The purpose of this study was to test various media and temperatures for the transportation of C. jejuni under aerobic conditions and to identify a cheap, effective and easy method that is appropriate for long distance transportation and can be applied by most veterinary/medical laboratories with a basic infrastructure. Materials and methods: We examined Mueller-Hinton (MH) agar with and w/o 2% horse blood and m-CCDA at room temperature and 2 AE 2 (SD) C under atmospheric conditions for survival of Campylobacter strains. Results: MH agar with 2% horse blood, suitable transport vials, and an optimum temperature of 2 AE 2 C provided survival of three Campylobacter type strains for at least one month under atmospheric conditions. This was validated by a transport test in which 101 isolates were shipped from Turkey to Austria. All isolates could be recultured and 97% survived more than one month in the transport medium. Conclusion: These findings indicate that the described approach is suitable for inter-laboratory transport of C. jejuni isolates.

Food safety challenges associated with traditional foods of Turkey

“…According to magnified risk factors (Table 3), the traditional Turkish dishes were categorized on Table 4. Risk estimation (categorization) for each dish was made by as well considering six different frequency of consumption scenarios based on statistical data shown on Table 2 (Omurtag et al, 2013). Risk degrees were stated by color, the darker the color the greater the risk for easy analysis.…”

“…Table 3. Factors considered in the estimation of the risk magnitude of foodborne pathogens (Omurtag et al, 2013).…”

Evaluation of the single and combined antibacterial efficiency of essential oils for controlling Campylobacter coli, Campylobacter jejuni, Escherichia coli, Staphylococcus aureus, and mixed cultures

“…Campylobacter spp. can be isolated from a variety of sources such as poultry and poultry products . Campylobacter is considered to be the most common bacterial cause of human gastroenteritis in the world .…”

“…can be isolated from a variety of sources such as poultry and poultry products. 23 Campylobacter is considered to be the most common bacterial cause of human gastroenteritis in the world. 24 Escherichia coli has been recognized traditionally as an indicator organism of fecal contamination of water and seafood.…”

Estudio de los caracteres epidemiológicos y control experimental de "Campylobacter jejuni" en el entorno de la producción de pollos para carne = Study of the epidemiological characteristics and experimental control of "Campylobacter jejuni" in the area of poultry for meat production

“…jejuni es una especie bacteriana muy sensible a las condiciones ambientales Se ha demostrado que varios factores, como el oxígeno, la temperatura o la composición media del medio, podrían afectar su supervivencia durante el transporte (Wang et al, 1983b). Por ello, para garantizar la viabilidad del microorganismo durante su transporte se deben utilizar condiciones particulares y medios específicos (Omurtag et al, 2011), sobre todo cuando se usan hisopos rectales, no solo por la pequeña cantidad de bacterias sino también por su mayor exposición al oxígeno (Palumbo, 1986).…”