Simple HPLC method for cefazolin determination in human serum – validation and stability testing

Kunicki, Paweł K.; Waś, Joanna

doi:10.1016/j.jchromb.2012.11.002

Cited by 20 publications

(14 citation statements)

References 28 publications

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“…The device with the heater was placed on the surface of 1.5 ml of freshly prepared PBS and a tiny magnetic stirrer bar was placed in PBS media, and different electrical voltage was applied to the conductive pattern to generate heat. The drug released during different voltages, temperature and time was measured by HPLC based on the method previously mentioned 45 , 46 . The surface temperature of the device measured by Welch Allyn 105801 Caretemp Touch Free Infrared Thermometer.…”

Section: Methodsmentioning

confidence: 99%

Biodegradable elastic nanofibrous platforms with integrated flexible heaters for on-demand drug delivery

Tamayol

Najafabadi

Mostafalu

et al. 2017

Sci Rep

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Delivery of drugs with controlled temporal profiles is essential for wound treatment and regenerative medicine applications. For example, bacterial infection is a key challenge in the treatment of chronic and deep wounds. Current treatment strategies are based on systemic administration of high doses of antibiotics, which result in side effects and drug resistance. On-demand delivery of drugs with controlled temporal profile is highly desirable. Here, we have developed thermally controllable, antibiotic-releasing nanofibrous sheets. Poly(glycerol sebacate)- poly(caprolactone) (PGS-PCL) blends were electrospun to form elastic polymeric sheets with fiber diameters ranging from 350 to 1100 nm and substrates with a tensile modulus of approximately 4-8 MPa. A bioresorbable metallic heater was patterned directly on the nanofibrous substrate for applying thermal stimulation to release antibiotics on-demand. In vitro studies confirmed the platform’s biocompatibility and biodegradability. The released antibiotics were potent against tested bacterial strains. These results may pave the path toward developing electronically controllable wound dressings that can deliver drugs with desired temporal patterns.

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Section: Methodsmentioning

confidence: 99%

Biodegradable elastic nanofibrous platforms with integrated flexible heaters for on-demand drug delivery

Tamayol

Najafabadi

Mostafalu

et al. 2017

Sci Rep

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“…[14][15][16][17][18][19] A simple liquid chromatography method with UV detection has been developed and fully validated for the determination of cefazolin in human plasma. [20] In addition, hyphenated techniques, such as liquid-chromatographytandem mass spectrometry (LC-MS/MS) have also been applied for the determination of cefazolin in human plasma using solidphase extraction (SPE) for sample preparation [21,22] and in human plasma and subcutaneous interstitial fluid of patients undergoing elective/semi-elective abdominal aortic aneurysm open repair surgery, [23] cefuroxime sodium in human plasma or serum [24,25] and cefuroxime lysine in rat brain [26] and in dog plasma. [27] Although the methods described include several successful approaches, studies quantifying transfer of cephalosporins into breast milk are rare in bibliography and most of them are quite old.…”

Section: Introductionmentioning

confidence: 99%

Quantification of three beta‐lactam antibiotics in breast milk and human plasma by hydrophilic interaction liquid chromatography/positive‐ion electrospray ionization mass spectrometry

Kiriazopoulos

Zaharaki

Vonaparti

et al. 2016

Drug Testing and Analysis

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The use of cephalosporins during breast feeding raises several issues, including the risk of drug exposure through breast milk for the infant. In this paper, a hydrophilic interaction liquid chromatography/positive ion electrospray mass spectrometric assay (HILIC/ESI-MS) was developed for the quantitation of cefuroxime, cefoxitin, and cefazolin in breast milk and human plasma. The assay was based on the use of small sample size, 25 μL of biological samples, following acetonitrile precipitation of proteins and filtration that enabled injection into the HILIC/ESI-MS system. All analytes and the internal standard, alfuzosin, were separated by using a ZIC®-HILIC analytical column (150.0 × 2.1 mm i.d., particle size 3.5 µm, 200 Å) with isocratic elution. The mobile phase was composed of a 6% 12.5 mM ammonium acetate water solution in acetonitrile and pumped at a flow rate of 0.25 mL min . The assay was linear over a concentration range of 0.2 to 5 µg mL and 0.4 to 20 µg mL for all the analytes in breast milk and in human plasma, respectively. Intermediate precision was found to be less than 4.2% over the tested concentration ranges. A run time of less than 12 min for each sample made it possible to analyze a large number of biological samples per day. The method is the first reported application of HILIC in the analysis of antibiotics in breast milk and human plasma and it can be used to support a wide range of clinical studies. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Cefazolin quantification has been previously reported using high performance liquid chromatographic (HPLC) [18] , [19] , [20] or liquid chromatographic–tandem mass spectrometric (LC–MS/MS) [21] , [22] , [23] , [24] , [25] approaches. However, none of the aforementioned methods have extensively evaluated the acceptability of cord blood as a matrix for cefazolin determination.…”

Section: Introductionmentioning

confidence: 99%

“…However, none of the aforementioned methods have extensively evaluated the acceptability of cord blood as a matrix for cefazolin determination. While several methods provide strategies for directly quantifying free drug concentration, only the methods published by Zhang et al [21] and Douglas et al [23] utilized tandem mass spectrometry as an analytical detector [18] , [19] , [20] , [22] . Here we present a comprehensive method describing the development and validation of LC–MS/MS methods for the quantification of total and free cefazolin via ultrafiltration in plasma and cord blood.…”

Section: Introductionmentioning

confidence: 99%

Bioanalytical development and validation of liquid chromatographic–tandem mass spectrometric methods for the quantification of total and free cefazolin in human plasma and cord blood

Crutchfield

Marzinke

2015

Practical Laboratory Medicine

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ObjectivesCefazolin is a commonly prescribed β-lactam antibiotic for prophylaxis against skin infections following surgery, including caesarean sections. Assessment of maternal and neonatal exposure is important for correlating drug concentrations to clinical outcomes. Thus, bioanalytical methods for the quantification of both total and free cefazolin in maternal plasma and cord blood can assist in the comprehensive evaluation of cefazolin exposure.Design and methodsSpecimen preparation for the measurement of total cefazolin was performed via protein precipitation with acetonitrile containing the internal standard cloxacillin. Ultrafiltration was used to isolate free cefazolin. Processed samples were analyzed on a Prelude SPLC system coupled to a TSQ triple quadrupole Vantage mass spectrometer. Methods were validated following FDA bioanalytical guidelines.ResultsThe analytical measuring ranges of these methods were 0.48–480 µg/mL and 0.048–48 µg/mL for total and free drug, respectively. Calibration curves were generated using 1/x2 weighted linear regression analysis. Total cefazolin demonstrated inter- and intra-assay precision of ≤20% at the LLOQ and ≤11.2% at other levels. Free cefazolin demonstrated inter- and intra-assay precision of ≤18.5% at the LLOQ and ≤12.6% at other levels, respectively. Accuracy (%DEV), carryover, matrix effects, recovery and stability studies were also acceptable based on FDA recommendations. Furthermore, it was demonstrated that samples prepared in cord blood can be accurately quantified from an adult plasma calibration curve, with recoveries ≤9.1% DIF and ≤11.9% DIF for total and free cefazolin, respectively.ConclusionsThe described LC–MS/MS methods allow for the measurement of total and free cefazolin in both plasma and cord blood.

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Simple HPLC method for cefazolin determination in human serum – validation and stability testing

Cited by 20 publications

References 28 publications

Biodegradable elastic nanofibrous platforms with integrated flexible heaters for on-demand drug delivery

Biodegradable elastic nanofibrous platforms with integrated flexible heaters for on-demand drug delivery

Quantification of three beta‐lactam antibiotics in breast milk and human plasma by hydrophilic interaction liquid chromatography/positive‐ion electrospray ionization mass spectrometry

Bioanalytical development and validation of liquid chromatographic–tandem mass spectrometric methods for the quantification of total and free cefazolin in human plasma and cord blood

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