Simple determination of betamethasone and chloramphenicol in a pharmaceutical preparation using a short monolithic column coupled to a sequential injection system

Abstract

This contribution describes the use of a new separation method based on a reversed-phase sequential injection chromatography (SIC) technique for simultaneous determination of chloramphenicol and betamethasone in pharmaceutical eye drops. A short monolithic column coupled with a sequential injection analysis (SIA) system enabled separation of two compounds in one step. A Chromolith Flash RP-18e, 25 x 4.6 mm column with a 5 mm precolumn (Merck, Germany) and a FIA1ab 3000 system (USA) with a 6-port selection valve and 5 mL syringe were used for sequential injection chromatographic separations in this study. The mobile phase used was acetonitrile-water (30:80, v/v), flow rate 0.48 mL/min; UV detection was at two wavelengths, i.e., 241 and 278 nm (absorption maxima of betamethasone and chloramphenicol, respectively). The basic validation parameters showed good results: linearity of determination for both compounds including internal standard (propylparaben) >0.999; repeatability of determination (RSD) in the range 0.8-1.7% at two different concentration levels, and detection limits in the range 0.5-1.0 mg/mL. The chromatographic resolution between compound peaks was greater than 2.1 and the analysis time was less than 8 min under optimal conditions. The developed sequential injection chromatography method was compared with the HPLC method and was found to be applicable for routine analysis of active compounds in pharmaceutical preparations.

Surfactant‐bound monolithic columns for CEC

“…Hence, the use of monolithic columns has increased and new stationary phases as well as column preparation procedure are being explored. Furthermore, a large number of new and attractive chromatographic applications have been developed in biological 5–11, environmental 12–14 and pharmaceutical 15–19 analysis, which may benefit from using monolithic column. In addition, the main advantage of CEC over micro HPLC is the electrically driven flow, which generates a flat flow profile and provides sharper peaks and better efficiencies for small molecule separations.…”

Advances in the analysis of steroid hormone drugs in pharmaceuticals and environmental samples (2004–2010)

“…Further theoretical and practical aspects [94] and application to formulations are the comparison of porous, fused-core and monolithic C18 columns for the determination of betamethasone valerate and its degradants in an ointment [95] and estradiol in a gel formulation [96]. Monoliths were also used for the assay of triamcinolone, prednisolone and dexamethasone in tablets [97] as well betamethasone [98] in eye drops together with chloramphenicol.…”

Fast and selective extraction of chloramphenicol from soil by matrix solid‐phase dispersion using molecularly imprinted polymer as dispersant

“…They can not only promote the generation and spread of drug resistant bacterial strains 3, but also cause potentially detrimental long‐term ecological effects 4. Chloramphenicol (CAP) is an antimicrobial agent used since 1950, active against both Gram‐positive and Gram‐negative bacteria 5. It inhibits bacterial protein synthesis by blocking the transfer of soluble ribonucleic acid to ribosome.…”