Similarity in the metabolic profile in macroscopically involved and un-involved colonic mucosa in patients with inflammatory bowel disease: an in vitro proton (1H) MR spectroscopy study

Sharma, Uma; Singh, Rajeev R.; Ahuja, Vineet; Makharia, Govind; Jagannathan, Naranamangalam R.

doi:10.1016/j.mri.2010.03.039

Cited by 36 publications

(34 citation statements)

References 32 publications

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“…Increased mannose was also observed in our DSS colitis mouse model, 40 while another group has found high levels of glucose in extracts of macroscopically uninvolved colonic mucosa of IBD patients. 33 In both CD and in UC, we noticed, relative to control subjects, a decrease in choline and its oxidized product betaine. Also other metabolomic studies of IBD have revealed a downregulation of choline, 20,32 which is an essential nutrient.…”

Section: Discussionmentioning

confidence: 74%

Quantitative Metabolomic Profiling of Serum, Plasma, and Urine by ¹H NMR Spectroscopy Discriminates between Patients with Inflammatory Bowel Disease and Healthy Individuals

Schicho

Shaykhutdinov²,

Ngo³

et al. 2012

J. Proteome Res.

213

170

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Serologic biomarkers for inflammatory bowel disease (IBD) have yielded variable differentiating ability. Quantitative analysis of a large number of metabolites is a promising method to detect IBD biomarkers. Human subjects with active Crohn’s disease (CD) and active ulcerative colitis (UC) were identified, and serum, plasma, and urine specimens were obtained. We characterized 44 serum, 37 plasma, and 71 urine metabolites by use of 1H NMR spectroscopy and “targeted analysis” to differentiate between diseased and non-diseased individuals, as well as between the CD and UC cohorts. We used multiblock principal component analysis and hierarchical OPLS-DA for comparing several blocks derived from the same “objects” (e.g., subject) to examine differences in metabolites. In serum and plasma of IBD patients, methanol, mannose, formate, 3-methyl-2-oxovalerate, and amino acids such as isoleucine were the metabolites most prominently increased, whereas in urine, maximal increases were observed for mannitol, allantoin, xylose, and carnitine. Both serum and plasma of UC and CD patients showed significant decreases in urea and citrate, whereas in urine, decreases were observed, among others, for betaine and hippurate. Quantitative metabolomic profiling of serum, plasma, and urine discriminates between healthy and IBD subjects. However, our results show that the metabolic differences between the CD and UC cohorts are less pronounced.

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Section: Discussionmentioning

confidence: 74%

Quantitative Metabolomic Profiling of Serum, Plasma, and Urine by ¹H NMR Spectroscopy Discriminates between Patients with Inflammatory Bowel Disease and Healthy Individuals

Schicho

Shaykhutdinov²,

Ngo³

et al. 2012

J. Proteome Res.

213

170

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“…255,272 Metabolic profiles of colonic biopsies in IBD has shown that there was little difference between inflamed and noninflamed tissue, but the metabolic profiles differed from that of healthy controls. 274 In summary, the rapid development of extensive datasets of the genetic potential resident within various gut microbiomes should soon result in the next wave of highimpact discoveries, which elucidate not only the structural, but also the functional attributes of the gut microbiota that contribute to health and/or disease. Such datasets should support the further establishment of systems biology approaches within select clinical studies, increasing the likelihood of dissecting the roles of the host, diet, and microbiology in gut health and disease.…”

Section: Metabolomics: Metabolitesmentioning

confidence: 99%

Characterization of the Gastrointestinal Microbiota in Health and Inflammatory Bowel Disease

Cruz

Prideaux

Wagner

et al. 2012

Inflammatory Bowel Diseases

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The enteric bacterial flora play a key role in maintaining health. Inflammatory bowel disease is associated with quantitative and qualitative alterations in the microbiota. Early characterization of the microbiota involved culture-dependent techniques. The advent of metagenomic techniques, however, allows for structural and functional characterization using culture-independent methods. Changes in diversity, together with quantitative alterations in specific bacterial species, have been identified. The functional significance of these changes, and their pathogenic role, remain to be elucidated.

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“…Higher α-glucose and lower amino acids, membrane components, lactate and succinate in active UC and CD compared to HC Lower lactate, glycerophosphorylcholine and myo-inositol in inactive UC and lower lactate in inactive CD compared to HC Lower formate in active UC vs active CD Sharma et al [62] 1 HNMR Colonic biopsies (inflamed and non-inflamed) UC (n = 12), CD (n = 9), controls (n = 25)…”

Section: Metabolomics In Ibsmentioning

confidence: 99%

“…Results from studies that analyzed serum/plasma or colonic mucosa cells indicate that both CD and UC have an impact on the amino acid metabolism [53,[58][59][60][61][62][63] . Several amino acids occurred in lower levels in colonic mucosal cells from IBD patients as compared to controls.…”

Section: Metabolomics In Ibdmentioning

confidence: 99%

Metabolomics as a diagnostic tool in gastroenterology

Preter¹,

Verbeke²

2013

WJGPT

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Metabolomics has increasingly been applied in addition to other "omic" approaches in the study of the pathophysiology of different gastrointestinal diseases. Metabolites represent molecular readouts of the cell status reflecting a physiological phenotype. In addition, changes in metabolite concentrations induced by exogenous factors such as environmental and dietary factors which do not affect the genome, are taken into account. Metabolic reactions initiated by the host or gut microbiota can lead to "marker" metabolites present in different biological fluids that allow differentiation between health and disease. Several lines of evidence implicated the involvement of intestinal microbiota in the pathogenesis of inflammatory bowel disease (IBD). Also in irritable bowel syndrome (IBS), a role of an abnormal microbiota composition, so-called dysbiosis, is supported by experimental data. These compositional alterations could play a role in the aetiology of both diseases by altering the metabolic activities of the gut bacteria. Several studies have applied a metabolomic approach to identify these metabolite signatures. However, before translating a potential metabolite biomarker into clinical use, additional validation studies are required. This review summarizes contributions that metabolomics has made in IBD and IBS and presents potential future directions within the field.

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Similarity in the metabolic profile in macroscopically involved and un-involved colonic mucosa in patients with inflammatory bowel disease: an in vitro proton (1H) MR spectroscopy study

Cited by 36 publications

References 32 publications

Quantitative Metabolomic Profiling of Serum, Plasma, and Urine by ¹H NMR Spectroscopy Discriminates between Patients with Inflammatory Bowel Disease and Healthy Individuals

Quantitative Metabolomic Profiling of Serum, Plasma, and Urine by ¹H NMR Spectroscopy Discriminates between Patients with Inflammatory Bowel Disease and Healthy Individuals

Characterization of the Gastrointestinal Microbiota in Health and Inflammatory Bowel Disease

Metabolomics as a diagnostic tool in gastroenterology

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Similarity in the metabolic profile in macroscopically involved and un-involved colonic mucosa in patients with inflammatory bowel disease: an in vitro proton (1H) MR spectroscopy study

Cited by 36 publications

References 32 publications

Quantitative Metabolomic Profiling of Serum, Plasma, and Urine by 1H NMR Spectroscopy Discriminates between Patients with Inflammatory Bowel Disease and Healthy Individuals

Quantitative Metabolomic Profiling of Serum, Plasma, and Urine by 1H NMR Spectroscopy Discriminates between Patients with Inflammatory Bowel Disease and Healthy Individuals

Characterization of the Gastrointestinal Microbiota in Health and Inflammatory Bowel Disease

Metabolomics as a diagnostic tool in gastroenterology

Contact Info

Product

Resources

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Quantitative Metabolomic Profiling of Serum, Plasma, and Urine by ¹H NMR Spectroscopy Discriminates between Patients with Inflammatory Bowel Disease and Healthy Individuals

Quantitative Metabolomic Profiling of Serum, Plasma, and Urine by ¹H NMR Spectroscopy Discriminates between Patients with Inflammatory Bowel Disease and Healthy Individuals