Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility: An in vitro study

Chen, Yi-Chuan; Pan, Li-Chern; Lai, Cheng Wei; Chien, Ying Shan; Wu, Tzong–Lin

doi:10.1016/j.repbio.2015.06.003

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…Glucose has, however, multiple roles in mature spermatozoa [42] and is known to rapidly affect sperm motility in vitro [43,44]. This is likely because mature human spermatozoa use glucose and fructose as primary source of ATP.…”

Section: Paralleled Shift In Sperm Motility and Tsrnamentioning

confidence: 99%

Human sperm displays rapid responses to diet

et al. 2019

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The global rise in obesity and steady decline in sperm quality are two alarming trends that have emerged during recent decades. In parallel, evidence from model organisms shows that paternal diet can affect offspring metabolic health in a process involving sperm tRNAderived small RNA (tsRNA). Here, we report that human sperm are acutely sensitive to nutrient flux, both in terms of sperm motility and changes in sperm tsRNA. Over the course of a 2-week diet intervention, in which we first introduced a healthy diet followed by a diet rich in sugar, sperm motility increased and stabilized at high levels. Small RNA-seq on repeatedly sampled sperm from the same individuals revealed that tsRNAs were up-regulated by eating a high-sugar diet for just 1 week. Unsupervised clustering identified two independent pathways for the biogenesis of these tsRNAs: one involving a novel class of fragments with specific cleavage in the T-loop of mature nuclear tRNAs and the other exclusively involving mitochondrial tsRNAs. Mitochondrial involvement was further supported by a similar up-regulation of mitochondrial rRNA-derived small RNA (rsRNA). Notably, the changes in sugarsensitive tsRNA were positively associated with simultaneous changes in sperm motility and negatively associated with obesity in an independent clinical cohort. This rapid response to a dietary intervention on tsRNA in human sperm is attuned with the paternal intergenerational metabolic responses found in model organisms. More importantly, our findings suggest shared diet-sensitive mechanisms between sperm motility and the biogenesis of tsRNA, which provide novel insights about the interplay between nutrition and male reproductive health.

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Section: Paralleled Shift In Sperm Motility and Tsrnamentioning

confidence: 99%

Human sperm displays rapid responses to diet

et al. 2019

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“…Spermatogoniawhich lie close to the basal membranedepend on blood glucose to fuel glycolytic processes, whilst later stage spermatocytes and spermatidslocated deeper within the testisrequire lactate secreted by Sertoli cells [75,76]. In contrast, spermatozoa are more adaptable on which substratesfor example glucose, fructose and pyruvatethey use for their energy metabolism, which is believed to be an adaptation to the varying availability of substrates in the male and female reproductive tracts [77][78][79][80][81]. Regardless, since spermatozoa mostly lack the ability to store energy, extracellular substrates for ATP production are essential for fuelling the axonemal dynein motors that results in forward motility (Fig.…”

Section: Dynamic Changes Of Sperm Sncrna During Spermatogenesismentioning

confidence: 99%

Male reproductive health and intergenerational metabolic responses from a small RNA perspective

Nätt

Öst

2020

J Intern Med

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The world has recently experienced a decline in male reproductive (e.g. sperm counts and motility) and metabolic (e.g. obesity and diabetes) health. Accumulated evidence from animal models also shows that the metabolic health of the father may influence the metabolic health in his offspring. Vectors for such paternal intergenerational metabolic responses (IGMRs) involve small noncoding RNAs (sncRNAs) that often increase in spermatozoa during the last days of maturation in the epididymis. We and others have shown that the metabolic state – depending on factors such as diet, obesity and physical exercise – may affect sperm quality and sperm sncRNA. Together, this suggests that there are overlapping aetiologies between the male metabolic syndrome, male factor infertility and intergenerational responses. In this review, we present a theoretical framework for an overlap of these aetiologies by exploring the advances in our understanding of the roles of sncRNA in spermatogenesis and offspring development. A special focus will lie on novel findings about tRNA‐derived small RNA (tsRNA), rRNA‐derived small RNA (rsRNA) and small mitochondrial RNA (mitoRNA), and their emerging roles in intergenerational metabolic and reproductive health.

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“…As previously described in a protocol from our lab [ 72 ], ICR mice purchased from Biolasco (I Lan, Taiwan) were bred in the Animal Center at Taipei Medical University according to the protocol (approval numbers LAC-2015-0265, LAC-2018-0345) and animal handling was according to the guidelines. Sperm were taken from the cauda epididymides of 12- to 16-week-old male mice and placed in buffer solution containing 120 mM NaCl, 1.99 mM KCl, 1.06 mM MgSO 4 ·7H 2 O, 0.3 mM NaH 2 PO 4 , 5.6 mM d-glucose, 18.4 mM sucrose, 10.9 mM HEPES, 1 M sodium pyruvate, and 1 M NaHCO3.…”

Section: Methodsmentioning

confidence: 99%

Phosphoproteomics and Bioinformatics Analyses Reveal Key Roles of GSK-3 and AKAP4 in Mouse Sperm Capacitation

Syifa

Yang

et al. 2020

IJMS

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Protein phosphorylation can induce signal transduction to change sperm motility patterns during sperm capacitation. However, changes in the phosphorylation of sperm proteins in mice are still incompletely understood. Here, capacitation-related phosphorylation in mouse sperms were firstly investigated by label-free quantitative (LFQ) phosphoproteomics coupled with bioinformatics analysis using ingenuity pathway analysis (IPA) methods such as canonical pathway, upstream regulator, and network analysis. Among 1632 phosphopeptides identified at serine, threonine, and tyrosine residues, 1050 novel phosphosites, corresponding to 402 proteins, were reported. Gene heatmaps for IPA canonical pathways showed a novel role for GSK-3 in GP6 signaling pathways associated with capacitation for 60 min. At the same time, the reduction of the abundant isoform-specific GSK-3α expression was shown by western blot (WB) while the LFQ pY of this isoform slightly decreased and then increased. The combined results from WB and LFQ methods explain the less inhibitory phosphorylation of GSK-3α during capacitation and also support the predicted increases in its activity. In addition, pAKAP4 increased at the Y156 site but decreased at the Y811 site in a capacitated state, even though IPA network analysis and WB analysis for overall pAKAP revealed upregulated trends. The potential roles of GSK-3 and AKAP4 in fertility are discussed.

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Silymarin and protein kinase A inhibitor modulate glucose-mediated mouse sperm motility: An in vitro study

Cited by 7 publications

References 28 publications

Human sperm displays rapid responses to diet

Human sperm displays rapid responses to diet

Male reproductive health and intergenerational metabolic responses from a small RNA perspective

Phosphoproteomics and Bioinformatics Analyses Reveal Key Roles of GSK-3 and AKAP4 in Mouse Sperm Capacitation

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