Silver nanoparticles of Albizia adianthifolia: the induction of apoptosis in human lung carcinoma cell line

Abstract

BackgroundSilver nanoparticles (AgNP), the most popular nano-compounds, possess unique properties. Albizia adianthifolia (AA) is a plant of the Fabaceae family that is rich in saponins. The biological properties of a novel AgNP, synthesized from an aqueous leaf extract of AA (AAAgNP), were investigated on A549 lung cells. Cell viability was determined by the MTT assay. Cellular oxidative status (lipid peroxidation and glutathione (GSH) levels), ATP concentration, caspase-3/-7, -8 and −9 activities were determined. Apoptosis, mitochondrial (mt) membrane depolarization (flow cytometry) and DNA fragmentation (comet assay) were assessed. The expression of CD95 receptors, p53, bax, PARP-1 and smac/DIABLO was evaluated by flow cytometry and/or western blotting.ResultsSilver nanoparticles of AA caused a dose-dependent decrease in cell viability with a significant increase in lipid peroxidation (5-fold vs. control; p = 0.0098) and decreased intracellular GSH (p = 0.1184). A significant 2.5-fold decrease in cellular ATP was observed upon AAAgNP exposure (p = 0.0040) with a highly significant elevation in mt depolarization (3.3-fold vs. control; p < 0.0001). Apoptosis was also significantly higher (1.5-fold) in AAAgNP treated cells (p < 0.0001) with a significant decline in expression of CD95 receptors (p = 0.0416). Silver nanoparticles of AA caused a significant 2.5-fold reduction in caspase-8 activity (p = 0.0024) with contrasting increases in caspase-3/-7 (1.7-fold vs. control; p = 0.0180) and −9 activity (1.4-fold vs. control; p = 0.0117). Western blots showed increased expression of smac/DIABLO (4.1-fold) in treated cells (p = 0.0033). Furthermore, AAAgNP significantly increased the expression of p53, bax and PARP-1 (1.2-fold; p = 0.0498, 1.6-fold; p = 0.0083 and 1.1-fold; p = 0.0359 respectively).ConclusionData suggests that AAAgNP induces cell death in the A549 lung cells via the mt mediated intrinsic apoptotic program. Further investigation is required to potentiate the use of this novel compound in cancer therapy trials.

Differential nanoreprotoxicity of silver nanoparticles in male somatic cells and spermatogonial stem cells

“…Mitochondrial permeability has been associated with various metabolic consequences, such as halted electron transport, generation of ROS, and decreased production of ATP. 90,91 Collectively, our results suggest that AgNPs of both sizes upregulate caspase mRNA; however, 10 nm particles produced a significantly stronger effect than the 20 nm particles, and TM3 cells were more sensitive than TM4 cells.…”

Comparative assessment of the apoptotic potential of silver nanoparticles synthesized by Bacillus tequilensis and Calocybe indica in&nbsp;MDA-MB-231 human breast cancer cells: targeting p53 for anticancer therapy

“…Figure 2A shows the XRD pattern obtained from the culture supernatant of B. tequilensis. The presence of peaks at 2θ values of 32.0° and 45.7° corresponds to (111) and (200) planes of silver, respectively. Thus, the XRD spectrum confirmed the crystalline structure of AgNPs.…”

Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties

“…Previous studies have shown that AgNPs have cytotoxic effects in different cell types and apoptosis-inducing properties. 20,25,41 In vitro apoptosis/necrosis assay Further experiments were conducted with fluorescein isothiocyanate-annexin V and PI staining and flow cytometric analysis. During early apoptosis, phosphatidyl serins (PS) in the membrane translocate to the outer leaflet of the cell membrane.…”

“…In addition, the rate of reduction of metal ions using plant extracts has been found to be much faster compared to microorganisms, and the stable formation of metallic NPs has been studied. 18 Several reports are available in the literature on the green synthesis of AgNPs using various plant extracts, including those from Plumbago zeylanica, 19 Albizia adianthifolia, 20 Pulicaria glutinosa, 21 Eucalyptus leucoxylon, 22 Acacia leucophloea, 23 Chrysanthemum indicum L., 24 Achillea biebersteinii, 25 and Lantana camara. 26 Therefore, many plant-based products have been used in the synthesis of AgNPs.…”