Silver Nanoparticles From Trianthema Portulacastrum: Green Synthesis, Characterization, Antibacterial and Anticancer Properties

Abstract

Objective: In this study, silver nanoparticles (SNPs) were synthesized using an aqueous extract of Trainthema portulacastrum and silver ions (Ag+) which have been proven against certain pathogenic bacterial strains and hepatocellular carcinoma (HepG2) cell line. Methods:The bio fabricated nanoparticles were confirmed by surface plasmon resonance which were characterized by biophysical measures utilizing the ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray, and transmission electron microscope (TEM), Fourier transform infrared spectroscopy, particle size analyzer, and X-ray diffraction. Antibacterial efficacy against Enterobacter aerogens, Proteus mirabilis, Escherichia coli, Staphylococcus epidermis, and Bacillus subtilis. The effect of SNPs tested against HepG2 and NIH/3T3 cell line exhibits a dose-dependent toxicity. Results and Conclusion:The SEM and TEM images confirmed the presence of spherical and hexagonal shape (0.3-4 µm) of nanocrystalline particles with the size range of 11.5-29.2 nm. The average particles size of SNPs is 190.3±17.0 nm. Antibacterial activity was carried out by agar well diffusion method against different pathogenic bacteria of which B. subtilis showed a significant zone of inhibition 8.66 mm and 12.0 mm for aqueous plant extract and synthesized SNPs. The effect of SNPs tested against HepG2 and NIH/3T3 cell line exhibits a dose-dependent toxicity. In case of HepG2, the cell viability was decreased to 50% (IC 50 ) at the concentration of 173.8±0.84 µg/mL. From the results, it can be concluded that the SNPs fabricated using green synthesis method will be a promising candidate in the biomedical field, due to its high bioactive properties.

Synthesis and Characterization of Silver Nanoparticles Using Bioreductant Andong Leaf Extract (Cordyline fruticosa (L) A. Chev.)

“…leaf extract indicates the presence of flavonoid and phenolic compounds, which function as bioreductants. This finding implies forming a layer of organic compounds on the surface of the silver nanoparticles, acting as stabilizing agents to prevent aggregation [26].…”