“…As a promising label-free fluorophore, DNA-templated Ag clusters (DNA/AgCs) have been confirmed with inherent advantages such as strong optical absorption, high quantum yield, tunable spectral emission, good biocompatibility, and low cost. − A variety of cytosine (C)-rich template sequences have been engineered for populating DNA/AgCs, on which Ag + ions preferably coordinate with C bases and then are reduced to form emissive cluster/nucleobase adducts. The change of the DNA template component and length determines the clusters’ number and geometry and therefore electronic structure and adjusts their color and brightness. , The clustering scaffolds and recognizable elements can be readily designed and programmed in diverse DNA-based probes, structures, or devices. − Once presenting a specific target, the molecular binding event is activated to guide their conformation switches; thus, the template sequences are liberated or locked at precise positions to develop or dissociate these fluorescent adducts, further modulating their emission spectra for tailored signaling modes. For example, Miao’s group reported chameleon Ag clusters through the directional disassembly of hairpin structures to construct logic operations or ratiometric sensing platforms. , Owing to the intrinsic built-in correction capable of minimizing false positive backgrounds, dual-color DNA/AgCs as ratiometric signal reporters are more desirable for accurately sensing a univariate target than a single cluster emitter. − By encoding the host templates in a shared DNA tweezer or three-way junction, we have explored the ratiometric fluorescence of bicolor Ag clusters under a target-stimulated conformation switch. − The ability of two clusters with configuration responsiveness from molecule-recognizing events enables accurate fluorescent assay of analytes at the ultralow level.…”