Silk gland fibroin from indian muga silkworm Antheraea assama as potential biomaterial

Kar, Subrata; Talukdar, Sarmistha; Pal, Shilpa; Nayak, Sunita; Paranjape, Pallavi; Kundu, Subhas C.

doi:10.1007/s13770-012-0008-6

Cited by 27 publications

(30 citation statements)

References 39 publications

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“…In A. assamensis, P. ricini and A. mylitta silk fibroin microparticles, the first weight loss was found at around 100-105 • C and the second weight loss at around 350-360 • C. However, in B. mori silk fibroin microparticles, the first weight loss peak was also observed at around 100-105 • C but the second weight loss peak was observed at 266 • C. Taken together, the enhanced thermal stability of the A. assamensis, P. ricini and A. mylitta fibroin microparticles than B. mori silk fibroin microparticles demonstrates a greater chemical stability of the non-mulberry fibroin based microparticles. These results are in agreement with the previous reports with films and fibers [21,51].…”

Section: Thermal Propertiessupporting

confidence: 94%

“…Attachment of the cells using A. assamensis microparticles was similar and comparable to that of P. ricini and A. mylitta microparticles, whereas B. mori microparticles incubated culture media showed sparse cellular attachment and distribution. The results are in agreement with the earlier reports using silk fibroin films of A. assamensis, A. mylitta and B. mori [21].…”

Section: Cell Attachment and Distributionsupporting

confidence: 93%

“…6A). This result was in agreement with the recent work carried out on A. assamensis and P. ricini silk fibroin film [21,51]. In contrast, B. mori silk fibroin microparticles showed a similar moisture peak at 105-109 • C but decomposition peak was at a lower temperature of 300 • C (Fig.…”

Section: Thermal Propertiessupporting

confidence: 92%

“…There are many factors, mostly matrix properties such as the presence of integrin binding sites, which affects the cell spreading [52]. After cell seeding, cells first attach themselves to the adhesive surfaces without immediately attaining their normal morphology, followed by spreading of cells after ascertaining the cell adhesive properties [21]. Either more or very less spreading indicates the poor health of the cells and causes deformation of cell morphology.…”

Section: In Vitro Cell Culture Cell Spreading and Cytocompatibilitymentioning

confidence: 99%

“…Silk fibroin (SF) derived from mulberry silkworm; Bombyx mori (B. mori) has been widely used as suitable matrices/substrates due to its high oxygen permeability, superior mechanical properties and excellent biocompatibility [10][11][12][13][14][15]. Additionally, there is a natural abundance of cell binding motifs, arginine-glycine-aspartic acid (RGD) in non-mulberry silk fibroin, which felicitates better cell attachment and proliferation [16][17][18][19][20][21]. The presence of RGD motifs in non-mulberry silk fibroin based matrices provide a substrate for stem cells differentiation and progenitor cells, which render it as a suitable biomaterial for variety of tissue engineering applications [5,22].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications

Bhardwaj

Rajkhowa

Wang

et al. 2015

International Journal of Biological Macromolecules

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Section: Thermal Propertiessupporting

confidence: 94%

Section: Cell Attachment and Distributionsupporting

confidence: 93%

Section: Thermal Propertiessupporting

confidence: 92%

Section: In Vitro Cell Culture Cell Spreading and Cytocompatibilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications

Bhardwaj

Rajkhowa

Wang

et al. 2015

International Journal of Biological Macromolecules

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Spectroscopic analysis of Muga silk nanoparticles synthesized by microwave method

Asapur

Banerjee

Sahare

et al. 2020

Biotech and App Biochem

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Muga silk nanoparticles (MSNP) were synthesized using a microwave‐assisted radiolysis method. The effect of microwave on the Muga protein secondary structures was analyzed. The evolution of the secondary structure from random coils to the β‐sheets was determined by using FTIR, circular dichroism and X‐ray diffraction techniques. The results showed that Muga silk fibroin protein contained the primary structure in silk‐I state. When the protein was irradiated with microwave, nanoparticle synthesis was possible having silk‐II state imparting crystallinity. The silk nanoparticles were characterized by a particle size analyzer and found to be of ~240 nm in size. The optical properties of these nanoparticles were studied by UV–vis. spectroscopy and photoluminescence. For studying thermal properties, differential scanning calorimetry was performed that revealed early glass transition, which could be attributed to the presence of water and proteins. It also revealed that nanoparticles are thermally stable. Such studies are important for understanding more about the MSNP and would be beneficial for their further wide applications.

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Evaluation of silk‐based bioink during pre and post 3D bioprinting: A review

et al. 2020

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During past few decades, the demand for the replacement of damaged organs is increasing consistently. This is due to the advancement in tissue engineering, which opens the possibility of regeneration of damaged organs or tissues into functional parts with the help of 3D bioprinting. Bioprinting technology presents an excellent potential to develop complex structures with precise control over cell suspension and structure. A brief description of different types of 3D bioprinting techniques, including inkjetbased, laser-based, and extrusion-based bioprinting is presented here. Due to innate advantageous features like tunable biodegradability, biocompatibility, elasticity and mechanical robustness, silk has carved a niche in the realm of tissue engineering. In this review article, the focus is to highlight the possible approach of exploring silk as bioink for fabrication of bioprinted implants using 3D bioprinting. This review discusses different type of degumming, dissolution techniques for extraction of proteins from different sources of silk. Different recently reported 3D bioprinting techniques suitable for silkbased bioink are further elaborated. Postprinting characterization of resultant scaffolds are also describe here. However, there is an astounding progress in 3D bioprinting technology, still there is a need to develop further the current bioprinting technology to make it suitable for generation of heterogeneous tissue construct. The possibility of utilizing the adhesive property of sericin to consider it as bioink is elaborated.

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Silk gland fibroin from indian muga silkworm Antheraea assama as potential biomaterial

Cited by 27 publications

References 39 publications

Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications

Milled non-mulberry silk fibroin microparticles as biomaterial for biomedical applications

Spectroscopic analysis of Muga silk nanoparticles synthesized by microwave method

Evaluation of silk‐based bioink during pre and post 3D bioprinting: A review

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