Silk fibroin in ocular tissue reconstruction

Harkin, Damien G.; George, Karina A.; Madden, Peter; Schwab, Ivan R.; Hutmacher, Dietmar W.; Chirilă, Traian V.

doi:10.1016/j.biomaterials.2010.12.041

Cited by 118 publications

(93 citation statements)

References 73 publications

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“…A number of biomaterials have been assessed as scaffolds (Feng et al, 2014) for transfer of corneal epithelial stem cells and transient-amplifying cells to the ocular surface (Joe & Yeung, 2014), and silk fibroin shows promise for corneo-limbal reconstruction (Harkin et al, 2011;Bray et al, 2012). We aimed to engineer a biomaterial suitable for ocular surface repair.…”

Section: Introductionmentioning

confidence: 99%

A novel pressed porous silicon-polycaprolactone composite as a dual-purpose implant for the delivery of cells and drugs to the eye

Irani

Tian

Wang

et al. 2015

Experimental Eye Research

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Abbreviations: BSS ophthalmic balanced salt solution; DMEM Dulbecco's Modified Eagle's Medium; EGF epidermal growth factor; FBS fetal bovine serum; FDA fluorescein diacetate; PBS phosphate buffered saline; PCL polycaprolactone; pSi nanostructured porous silicon; SEM scanning electron microscopy; TEM transmission electron microscopy. To investigate loading of large-molecule biologics, murine BALB/c 3T3 cells, responsive to epidermal growth factor, insulin and transferrin, were seeded on composite materials. The cells showed significantly more proliferation at 48 h when seeded on composites loaded with these biologics, than on unloaded composites. No cell proliferation was observed on PCL alone, indicating the biologics had loaded into the pSi microparticles. Drug release, measured by ELISA for insulin, indicated a burst followed by a slower, continuous release over six days. When implanted under the rat conjunctiva, the most promising composite material did not cause significant neovascularization but did elicit a macrophage and mild foreign body

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Section: Introductionmentioning

confidence: 99%

A novel pressed porous silicon-polycaprolactone composite as a dual-purpose implant for the delivery of cells and drugs to the eye

Irani

Tian

Wang

et al. 2015

Experimental Eye Research

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“…While little of the native full-length protein remains following processing, the resulting peptide solutions can subsequently be used to generate a variety of stable and biocompatible materials including freestanding membranes, electro-spun fibers and porous sponges [24]. Of these three materials, the freestanding BMSF membranes have received most attention for potential use in corneal tissue reconstruction owing to their high degree of transparency and general handling properties [25][26][27][28].…”

Section: • Silk Fibroin Based Constructsmentioning

confidence: 99%

Recent advances in the design of artificial corneas

Griffith

Harkin

2014

Current Opinion in Ophthalmology

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Purpose of review: Artificial corneas are being developed to meet a shortage of donor corneas as well as to address cases where allografting is contraindicated. A range of artificial corneas has been developed. Here we review several newer designs and especially those inspired by naturally occurring biomaterials found with the human body and elsewhere. Summary:More recent attempts at making artificial corneas have taken a more nature-based or nature-inspired approach. Several will in the near future be likely to be available clinically.

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“…This leaves a fibrous material that can be dissolved and then processed into many forms including transparent films with mechanical properties and degradation behavior that can be tailored for the specific application. [34] Corneal epithelial cells have been shown to attach and grow on fibroin films in vitro but that the presence of serum is essential and for primary cells a feeder-cell layer may be required suggesting that specific cell adhesive molecules may be required to aid cell attachment to the surface. An alternative use of silk fibroin has been as a reinforcing element in collagen-based membranes.…”

Section: Other Natural Polymers: Silks and Keratinmentioning

confidence: 99%

Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

Williams

Lace

Kennedy

et al. 2018

Adv Healthcare Materials

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The role of biomaterials in tissue engineering and regenerative medicine strategies to treat vision loss associated with damage to tissues in the anterior segment of the eye has been studied for several years. This has mostly involved replacement and support for the cornea and conjunctiva. These are complex tissues with specific functional requirements for different parts of the tissue. Amniotic membrane (AM) is used in clinical practice to transplant autologous or allogenic cells to the corneal surface. Fibrin gels have also progressed to clinical use under specific conditions. Alternatives to AM such as collagen gels, other natural materials, for example keratin and silks, and synthetic polymers have received considerable attention in laboratory and animal studies. This experience is building a body of evidence to demonstrate the potential of tissue engineering and regenerative medicine in corneal and conjunctival reconstruction and can also lead to other applications in the anterior segment of the eye, for example, the trabecular meshwork. There is a real clinical need for new procedures to overcome vision loss but there are also opportunities for developments in ocular applications to lead to biomaterials innovations for use in other clinical areas.

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Silk fibroin in ocular tissue reconstruction

Cited by 118 publications

References 73 publications

A novel pressed porous silicon-polycaprolactone composite as a dual-purpose implant for the delivery of cells and drugs to the eye

A novel pressed porous silicon-polycaprolactone composite as a dual-purpose implant for the delivery of cells and drugs to the eye

Recent advances in the design of artificial corneas

Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

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