Silencing of the Wheat Protein Phosphatase 2A Catalytic Subunit TaPP2Ac Enhances Host Resistance to the Necrotrophic Pathogen Rhizoctonia cerealis

Zhu, Xinna; Wang, Yuanyuan; Su, Zhenqi; Lv, Liangjie; Zhang, Zengyan

doi:10.3389/fpls.2018.01437

Cited by 22 publications

(23 citation statements)

References 49 publications

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“…For wheat, a PP2A catalytic subunit belonging to family II (TaPP2Ac) negatively regulates the expression of genes for the antioxidant enzymes CAT and APX2 as well of the pathogenesis response protein PR2. Infection with the fungal pathogen Rhizoctonia cerealis increases the expression of this PP2Ac and exogenous H 2 O 2 application resembles this effect [39]. As we will see for pathway 3 [29], changes of PP2Ac activities that are due to misfunctioning of B regulatory subunits have partially different effects in ROS signaling, which underlines that the relevant involvement of this phosphatase depends largely on the type of activity change and subcellular localization of these effects.…”

Section: The Involvement Of Pp2a In Oxidative Stress Pathwaysmentioning

confidence: 97%

The Role of Serine-Threonine Protein Phosphatase PP2A in Plant Oxidative Stress Signaling—Facts and Hypotheses

Máthé

Garda

Freytag

et al. 2019

IJMS

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Abiotic and biotic factors induce oxidative stress involving the production and scavenging of reactive oxygen species (ROS). This review is a survey of well-known and possible roles of serine-threonine protein phosphatases in plant oxidative stress signaling, with special emphasis on PP2A. ROS mediated signaling involves three interrelated pathways: (i) perception of extracellular ROS triggers signal transduction pathways, leading to DNA damage and/or the production of antioxidants; (ii) external signals induce intracellular ROS generation that triggers the relevant signaling pathways and (iii) external signals mediate protein phosphorylation dependent signaling pathway(s), leading to the expression of ROS producing enzymes like NADPH oxidases. All pathways involve inactivation of serine-threonine protein phosphatases. The metal dependent phosphatase PP2C has a negative regulatory function during ABA mediated ROS signaling. PP2A is the most abundant protein phosphatase in eukaryotic cells. Inhibitors of PP2A exert a ROS inducing activity as well and we suggest that there is a direct relationship between these two effects of drugs. We present current findings and hypotheses regarding PP2A-ROS signaling connections related to all three ROS signaling pathways and anticipate future research directions for this field. These mechanisms have implications in the understanding of stress tolerance of vascular plants, having applications regarding crop improvement.

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Section: The Involvement Of Pp2a In Oxidative Stress Pathwaysmentioning

confidence: 97%

The Role of Serine-Threonine Protein Phosphatase PP2A in Plant Oxidative Stress Signaling—Facts and Hypotheses

Máthé

Garda

Freytag

et al. 2019

IJMS

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“…Recently, Zhu et al (2018) demonstrated the importance of PP2A in host-necrotroph interactions in crop species. Silencing of a wheat (Triticum aestivum) PP2A catalytic subunit conferred resistance against the necrotrophic fungus Rhizoctonia cerealis, the causative agent of wheat sharp eyespot disease.…”

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confidence: 99%

“…Silencing of a wheat (Triticum aestivum) PP2A catalytic subunit conferred resistance against the necrotrophic fungus Rhizoctonia cerealis, the causative agent of wheat sharp eyespot disease. The resistance phenotype was accompanied by increased transcript abundance for the wheat pathogenesis-related (PR) protein PR2 and antioxidant enzymes (Zhu et al, 2018). However, the exact molecular mechanisms by which PP2A contributes to plant resistance against necrotrophic fungal pathogens remain unresolved.…”

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confidence: 99%

PROTEIN PHOSPHATASE 2A-B′γ Controls Botrytis cinerea Resistance and Developmental Leaf Senescence

et al. 2019

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Plants optimize their growth and survival through highly integrated regulatory networks that coordinate defensive measures and developmental transitions in response to environmental cues. Protein phosphatase 2A (PP2A) is a key signaling component that controls stress reactions and growth at different stages of plant development, and the PP2A regulatory subunit PP2A-B9g is required for negative regulation of pathogenesis responses and for maintenance of cell homeostasis in short-day conditions. Here, we report molecular mechanisms by which PP2A-B9g regulates Botrytis cinerea resistance and leaf senescence in Arabidopsis (Arabidopsis thaliana). We extend the molecular functionality of PP2A-B9g to a protein kinase-phosphatase interaction with the defense-associated calcium-dependent protein kinase CPK1 and present indications this interaction may function to control CPK1 activity. In presenescent leaf tissues, PP2A-B9g is also required to negatively control the expression of salicylic acid-related defense genes, which have recently proven vital in plant resistance to necrotrophic fungal pathogens. In addition, we find the premature leaf yellowing of pp2a-b9g depends on salicylic acid biosynthesis via SALICYLIC ACID INDUCTION DEFICIENT2 and bears the hallmarks of developmental leaf senescence. We propose PP2A-B9g agedependently controls salicylic acid-related signaling in plant immunity and developmental leaf senescence.

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“…The expression of PRs genes up-regulated in the resistance cultivar than in susceptible wheat cultivar upon infection by Bipolaris sorokiniana and T. controversa [ 9 , 31 ]. The silencing or overexpression of TaPR-5 and TaLHY genes decrease or increase the resistance level against plant pathogens [ 30 , 61 , 62 ]. Here in the qRT-PCR analysis results revealed that infection with T. controversa triggers the expression levels of PRs genes ( TaPR-4 and TaPR-5 ) and TaLHY gene more in resistant and moderately resistance cultivars than in the susceptible cultivar.…”

Section: Discussionmentioning

confidence: 99%

Wheat Varietal Response to Tilletia controversa J. G. Kühn Using qRT-PCR and Laser Confocal Microscopy

Chen

Muhae-Ud-Din

Liu

et al. 2021

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Tilletia controversa J. G. Kühn is a causal organism of dwarf bunt in wheat. Understanding the interaction of wheat and T. controversa is of practical and scientific importance for disease control. In this study, the relative expression of TaLHY and TaPR-4 and TaPR-5 genes was higher in a resistant (Yinong 18) and moderately resistant (Pin 9928) cultivars rather than susceptible (Dongxuan 3) cultivar at 72 h post inoculation (hpi) with T. controversa. Similarly, the expression of defensin, TaPR-2 and TaPR-10 genes was observed higher in resistant and moderately resistant cultivars after exogenous application of phytohormones, including methyl jasmonate, salicylic acid, and abscisic acid. Laser confocal microscopy was used to track the fungal hyphae in the roots, leaves, and tapetum cells, which of susceptible cultivar were infected harshly by T. controversa than moderately resistant and resistant cultivars. There were no fungal hyphae in tapetum cells in susceptible cultivar after methyl jasmonate, salicylic acid and abscisic acid treatments. Moreover, after T. controversa infection, the pollen germination was of 80.06, 58.73, and 0.67% in resistant, moderately resistant and susceptible cultivars, respectively. The above results suggested that the use using of resistant cultivar is a good option against the dwarf bunt disease.

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Silencing of the Wheat Protein Phosphatase 2A Catalytic Subunit TaPP2Ac Enhances Host Resistance to the Necrotrophic Pathogen Rhizoctonia cerealis

Cited by 22 publications

References 49 publications

The Role of Serine-Threonine Protein Phosphatase PP2A in Plant Oxidative Stress Signaling—Facts and Hypotheses

The Role of Serine-Threonine Protein Phosphatase PP2A in Plant Oxidative Stress Signaling—Facts and Hypotheses

PROTEIN PHOSPHATASE 2A-B′γ Controls Botrytis cinerea Resistance and Developmental Leaf Senescence

Wheat Varietal Response to Tilletia controversa J. G. Kühn Using qRT-PCR and Laser Confocal Microscopy

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