Silencing of DLGAP5 by siRNA Significantly Inhibits the Proliferation and Invasion of Hepatocellular Carcinoma Cells

Liao, Weijia; Liu, Weilong; Yuan, Qing; Liu, Xing; Ou, Ying; He, Songqing; Yuan, Shengguang; Li, Qin; Chen, Qian; Nong, Kate; Mei, Ming-hui; Huang, Jian

doi:10.1371/journal.pone.0080789

Cited by 69 publications

(57 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This protein is relatively unstudied in tumor biology, but recently was shown to have a potential role in hepatocellular cancer. Liao et al demonstrated overexpression of DLGAP5 mRNA to be common in tumor samples from liver cancer patients, and that in vitro RNA-interference mediated silencing of DLGAP5 inhibited proliferation and invasion (38). One of the novel, ATC-specific underexpressed genes was C2orf40, which encodes the esophageal cancer-related gene 4 (ECRG4) protein, a tumor suppressor affecting cancer cell migration, invasion and cell cycle regulation and downregulated in esophageal and breast cancer (39,40).…”

Section: Discussionmentioning

confidence: 99%

Cell Cycle M-Phase Genes Are Highly Upregulated in Anaplastic Thyroid Carcinoma

Weinberger

Ponny

et al. 2017

Thyroid

View full text Add to dashboard Cite

Background: Anaplastic thyroid carcinoma (ATC) accounts for only 3% of thyroid cancers, yet strikingly, it accounts for almost 40% of thyroid cancer deaths. Currently, no effective therapies exist. In an effort to identify ATC-specific therapeutic targets, we analyzed global gene expression data from multiple studies to identify ATC-specific dysregulated genes. Methods: The National Center for Biotechnology Information Gene Expression Omnibus database was searched for high-throughput gene expression microarray studies from human ATC tissue along with normal thyroid and/or papillary thyroid cancer (PTC) tissue. Gene expression levels in ATC were compared with normal thyroid or PTC using seven separate comparisons, and an ATC-specific gene set common in all seven comparisons was identified. We investigated these genes for their biological functions and pathways. Results: There were three studies meeting inclusion criteria, (including 32 ATC patients, 69 PTC, and 75 normal). There were 259 upregulated genes and 286 downregulated genes in ATC with at least two-fold change in all seven comparisons. Using a five-fold filter, 36 genes were upregulated in ATC, while 40 genes were downregulated. Of the 10 top globally upregulated genes in ATC, 4/10 (MMP1, ANLN, CEP55, and TFPI2) are known to play a role in ATC progression; however, 6/10 genes (TMEM158, CXCL5, E2F7, DLGAP5, MME, and ASPM) had not been specifically implicated in ATC. Similarly, 3/10 (SFTA3, LMO3, and C2orf40) of the most globally downregulated genes were novel in this context, while 7/10 genes (SLC26A7, TG, TSHR, DUOX2, CDH1, PDE8B, and FOXE1) have been previously identified in ATC. We experimentally validated a significant correlation for seven transcription factors (KLF16, SP3, ETV6, FOXC1, SP1, EGFR1, and MAFK) with the ATC-specific genes using microarray analysis of ATC cell lines. Ontology clustering of globally altered genes revealed that ''mitotic cell cycle'' is highly enriched in the globally upregulated gene set (44% of top upregulated genes, p-value <10 -30 ). Conclusions: By focusing on globally altered genes, we have identified a set of consistently altered biological processes and pathways in ATC. Our data are consistent with an important role for M-phase cell cycle genes in ATC, and may provide direction for future studies to identify novel therapeutic targets for this disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell Cycle M-Phase Genes Are Highly Upregulated in Anaplastic Thyroid Carcinoma

Weinberger

Ponny

et al. 2017

Thyroid

View full text Add to dashboard Cite

show abstract

“…cDNA sample mixed with 20 μl Master mix (SYBR ® Green PCR Master Mix, Applied Biosystems) was amplified using the ABI Prism 7500 Sequence Detector System Applied Biosystems (Foster City, CA, USA) with the following reactive conditions: incubation at 95°C for 10 min; then 40 cycles of denaturation at 95°C for 2 sec, annealing at 55°C for 5 sec, and extension at 72°C for 15 sec. Relative DEPDC1 mRNA expression was calculated according to our previous report (Liao et al, 2013).…”

Section: 10917 Dep Domain Containing 1 Is a Novel Diagnostic Marker mentioning

confidence: 99%

DEP Domain Containing 1 is a Novel Diagnostic Marker and Prognostic Predictor for Hepatocellular Carcinoma

Yuan

Liao²,

Yang³

et al. 2015

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

“…The mean Ct value for β-actin was subtracted from the mean Ct value for RHAMM in each sample using the following formula: RHAMM ΔCt = (mean RHAMM Ct -mean β-actin Ct). The fold-change (2 -RHAMM ΔΔCt ) of the RHAMM expression level relative to the β-actin expression level was calculated for each sample (19).…”

Section: Reverse Transcription-quantitative Polymerase Chain Reactionmentioning

confidence: 99%

Upregulation of hyaluronan-mediated motility receptor in hepatocellular carcinoma predicts poor survival

Liao

Li³

et al. 2015

Oncology Letters

Self Cite

View full text Add to dashboard Cite

Abstract. Hepatocellular carcinoma (HCC) is one of the most common types of cancer across the world. Hyaluronic acid (HA) has been reported to serve an important role in tumor extension, progression, migration and invasion. In addition, the receptor for HA-mediated motility (RHAMM) has been demonstrated to be overexpressed in different types of cancer. However, whether the upregulation of RHAMM contributes to hepatocarcinogenesis of HCC remains unclear. The present study examined the RHAMM expression in 187 HCC patients by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC). RHAMM expression was significantly upregulated in liver cancer tissues compared with that observed in adjacent normal liver tissues. The IHC analysis demonstrated that RHAMM was overexpressed in 18 (72.0%) of the 25 HCC tissues. Furthermore, overexpression of RHAMM was associated with tumor-node-metastasis (TNM), the presence of vascular invasion and recurrence. Notably, the present study indicated that the overexpression of RHAMM was closely associated with the shorter disease-free and overall survival, so it may be a potential independent predictor for disease-free and overall survival of HCC patients. In conclusion, the upregulation of RHAMM is associated with HCC progression and prognosis; and it may be a potential independent predictor of disease-free and overall survival of HCC following surgical resection. IntroductionLiver cancer is one of the most common neoplasms worldwide, and hepatocellular carcinoma (HCC) is the most prevalent type of liver cancer and accounts for 70-85% of all cases (1), ranking the sixth most common malignant tumors worldwide (2). More than 600,000 people lose their lives due to HCC every year (3), and HCC is the third most frequent cause of cancer-associated mortality (4). Previous studies have demonstrated that the incidence rate of HCC has been increasing. Surgical resection and liver-transplantation are superior therapeutic methods for HCC compared with other treatments, including radiofrequency ablation (RFA), transarterial chemoembolization (TACE) and chemotherapy, the treatment outcomes remain unsatisfactory owing to the high recurrence rate and high fatality rate of HCC (2). If HCC was detected and diagnosed earlier, surgical resection and liver-transplantation would result in an improved curative effect, therefore improving the disease-free and overall survival. The serum levels of α-fetoprotein (AFP) and ultrasonography (US) are widely used to screen for and assess HCC throughout the world. However, the sensitivity and specificity of AFP for HCC diagnosis and surveillance have certain limitations; US depends on the operator's skill, and clinicians are not always able to distinguish HCC clearly from other nodules (5). Consequently, novel potential serum biomarkers are required for prognosis and metastatic recurrence of HCC.Hyaluronic acid (HA) is an extracellular matrix (ECM) polymer and is frequently localized in the stroma of solid tumors, which is s...

show abstract

Silencing of DLGAP5 by siRNA Significantly Inhibits the Proliferation and Invasion of Hepatocellular Carcinoma Cells

Cited by 69 publications

References 23 publications

Cell Cycle M-Phase Genes Are Highly Upregulated in Anaplastic Thyroid Carcinoma

Cell Cycle M-Phase Genes Are Highly Upregulated in Anaplastic Thyroid Carcinoma

DEP Domain Containing 1 is a Novel Diagnostic Marker and Prognostic Predictor for Hepatocellular Carcinoma

Upregulation of hyaluronan-mediated motility receptor in hepatocellular carcinoma predicts poor survival

Contact Info

Product

Resources

About