Alexander Böser, Hannes C.A. Drexler, Hanna Reuter, Henning Schmitz, Guangming Wu, Hans R. Schöler, Luca Gentile, Kerstin Bartscherer
Abstract:Planarian regeneration depends on the presence of pluripotent stem cells in the adult. We developed an in vivo stable isotope labeling by amino acids in cell culture (SILAC) protocol in planarians to identify proteins that are enriched in planarian stem cells. Through a comparison of SILAC proteomes of normal and stem cell-depleted planarians and of a stem cell-enriched population of sorted cells, we identified hundreds of stem cell proteins. One of these is an ortholog of nuclear receptor coactivator-5 (Ncoa5…
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