Sika deer antler protein against acetaminophen-induced nephrotoxicity by activating Nrf2 and inhibition FoxO1 via PI3K/Akt signaling

Ruan, Haonan; Luo, Jiacheng; Wang, Lulu; Wang, Jing; Wang, Zhenyi; Zhang, Jing

doi:10.1016/j.ijbiomac.2019.08.164

Cited by 17 publications

(26 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are presented as means ± SEM. The analyses were performed using SPSS 18.0 and the differences were compared with one-way analysis followed by Dunnett's multiple comparison test P < 0.05 was regarded as statistically significant.…”

Section: Discussionmentioning

confidence: 99%

“…Extraction and separation of SDAPR based on previous methods 18 . The content of SDAPR was 91.89% by Bradford method.…”

Section: Methodsmentioning

confidence: 99%

“…Following Ruan's method of publishing a paper in 2019 18 , the Native-PAGE gel was cut into thin slices for further identification. The samples were completely decolorized, lyophilized, 40 μL trypsin was added, and treated at 37 °C for 16–18 h. Analysis of samples using liquid chromatography-mass spectrometry/mass spectrometry (Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…It is reported that SDA extractive can inhibit cisplatin-induced kidney injury 17 . The results of the previous studies found that Sika deer antler protects (SDAP) against acetaminophen-induced acute kidney injury by activating Nrf2/HO-1 pathway 18 .…”

Section: Introductionmentioning

confidence: 97%

“…Medium volumes of liquids A and B were mixed, mixed and added to the surface of the membrane, transferred to a gel imaging analyzer, and exposed in a chemical light-sensitive mode. After exporting photos in TIF format, analyze the optical density of each band under ImageJ software 18 .…”

mentioning

confidence: 99%

See 4 more Smart Citations

Purified Sika deer antler protein attenuates GM-induced nephrotoxicity by activating Nrf2 pathway and inhibiting NF-κB pathway

Wang

Wang³

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Although gentamicin is widely used as an antibiotic in clinical practice, it also has some side-effects, such as acute kidney injury, which is a common condition caused by the abuse of gentamicin. Sika deer antler protein (SDAPR) can antagonize drug-induced AKI. Since SDAPR is recognized as an effective part of velvet antler, its components were further separated. Two components named SDAP1 and SDAP2 were obtained. The protective effects of SDAPR, SDAP1 and SDAP2 on GM-induced cytotoxicity to HEK293 and its potential mechanisms were studied. MTT and xCELLigence Real-Time cell analysis showed that SDAPR, SDAP1 and SDAP2 could protect HEK293 cells from GM toxicity. Similarly, SDAPR, SDAP1 and SDAP2 can reduce ROS level, reduce oxidative stress and improve inflammation Further studies have shown that SDAPR, SDAP1 and SDAP2 upregulate the Nrf2/HO-1 pathway by increasing the expression of Nrf2 and HO-1, and down-regulate the NF-κB pathway by reducing the protein expression of NF-κB. Annexin V/PI flow cytometry and Hoechst 33258 staining showed that SDAPR, SDAP1 and SDAP2 inhibited GM-induced apoptosis in HEK293 cells. Western blot analysis showed SDAPR, SDAP1 and SDAP2 decreased expression level of Bax and Cleaved-caspase-3, and increased the expression level of Bcl-2. In addition, we examined the feasibility of SDAP1 and SDAP1 to avoid kidney injury in a GM mouse model. In conclusion, SDAPR, SDAP1 and SDAP2 can be used to prevent GM-induced HEK293 cytotoxicity, probably because they have strong anti-oxidative stress, anti-inflammatory and anti-apoptotic effects. And SDAP1 and SDAP2 can inhibit GM-induced acute kidney injury in mice.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Extraction and separation of SDAPR based on previous methods 18 . The content of SDAPR was 91.89% by Bradford method.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

mentioning

confidence: 99%