Signature microRNAs and long noncoding RNAs in laryngeal cancer recurrence identified using a competing endogenous RNA network

Tang, Zhengyi; Wei, Gan-Guan; Zhang, Longcheng; Xu, Zhinan

doi:10.3892/mmr.2019.10143

Cited by 17 publications

(24 citation statements)

References 62 publications

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“…Tang et al identified that low expressions of Emx2os are closely associated with longer DSF and OS ratio in LSCC patients based on in silico analysis of databases. They also indicated that Emx2os regulates the expression of CALCA and GABRG2 through miR-124 [ 70 ]. We have also observed that high expressions of snaR and SNHG1 was correlated with a favorable OS in HNSCC patients.…”

Section: Discussionmentioning

confidence: 99%

Liquid lncRNA Biopsy for the Evaluation of Locally Advanced and Metastatic Squamous Cell Carcinomas of the Head and Neck

Łasińska

Kolenda

Guglas

et al. 2020

JPM

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Background: Long non-coding RNA (lncRNA) are RNA molecules that are more than 200 nucleotides long and have the ability to modify the activity of genes. They can be found in both healthy and cancer tissues, as well as in plasma, saliva and other bodily fluids. They can also be used as biomarkers of early detection, prognosis and chemotherapy resistance in several cancer types. Treatment of head and neck squamous cell carcinoma (HNSCC) patients with locally advanced disease is still difficult, and choice of treatment should be based on more precise and available biomarkers, such as those obtained from a liquid biopsy. For improvement of treatment efficacy, identification and clinical implementation of new biomarkers are of the utmost importance. Methods: Plasma samples drawn before (p1) and three cycles post (p2) (TPF: docetaxel, cisplatin, 5-fluorouracil/PF: cisplatin, 5-fluorouracil) chemotherapy from 53 HNSCC patients (17 with locally advanced and 36 with metastatic disease) and 14 healthy volunteers were studied. Expression levels of 90 lncRNA expression were analyzed using the qRT-PCR method, and the obtained results were compared between proper groups. Statistical analyses were carried out using Jupyter Notebooks (5.7.2), Python (ver. 3.6) and GraphPad Prism 8. Results: The study demonstrated the differences between the expressions of several lncRNA in cancer patients’ and healthy volunteers’ plasma, as well as between locally advanced and metastatic patients’ groups. A correlation between the response to systemic therapy and lncRNA expression levels was observed. Patients with a (high/low) expression of Alpha 250 and Emx2os showed statistically significant differences in progression free survival (PFS), as well as for overall survival (OS) depending on the level of Alpha 250, snaR, SNHG1. The univariate and multivariate Cox regression model showed Alpha 250 as the best prognostic factor for HNSCC patients. Conclusions: Liquid biopsies based on lncRNAs are promising diagnostic tools that can be used to differentiate between those with cancer and healthy individuals. Additionally, they can also serve as biomarkers for chemotherapy resistance. An identified, circulating lncRNA Alpha 250 seems to prove the best prognostic biomarker, associated with extended PFS and OS, and should be validated in a larger cohort in the future.

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Section: Discussionmentioning

confidence: 99%

Liquid lncRNA Biopsy for the Evaluation of Locally Advanced and Metastatic Squamous Cell Carcinomas of the Head and Neck

Łasińska

Kolenda

Guglas

et al. 2020

JPM

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“…LINC00488 functions as a ceRNA to regulate hepatocellular carcinoma cell proliferation and angiogenesis through miR-330-5 (17). Coding RNAs interact with non-coding RNAs through microRNA (miRNA/miR) response elements, thus influencing cellular communication, transcription regulation and biological functions (16)(17)(18). The bioinformatics analysis identified that miRNA-485-5p is the downstream gene of LINC00488.…”

Section: Introductionmentioning

confidence: 99%

LINC00488 stimulates the progression of esophageal cancer by targeting microRNA‑485‑5p

Yan

2020

Oncol Lett

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Esophageal cancer is the eighth most prevalent malignancy in the world and China has a high incidence of esophageal cancer. Previous studies have identified that LINC00488 is an oncogene; however, its role in esophageal cancer remains unclear. The present study detected the expression and biological functions of LINC00488 in the progression of esophageal cancer. LINC00488 levels in 45 esophageal cancer and matched paracancerous tissues were detected. The association between LINC00488 level, clinical indexes and overall survival rate of patients with esophageal cancer was analyzed. Using Cell Counting Kit-8, Transwell and wound healing assays, the influence of LINC00488 on the biological functions of OE19 and OE33 cells were assessed. The target gene of LINC00488, microRNA-485-5p (miRNA-485-5p), was predicted using bioinformatics databases. In addition, the role of miRNA-485-5p in the progression of esophageal cancer was evaluated using rescue experiments. LINC00488 was upregulated in esophageal cancer tissues and cell lines. A high level of LINC00488 was associated with lymphatic and distant metastasis and poor prognosis in patients with esophageal cancer. Silencing LINC00488 attenuated the viability, migration and wound healing of OE19 and OE33 cells. miRNA-485-5p was downregulated in esophageal cancer and low expression levels predicted a poor prognosis in these patients. In addition, miRNA-485-5p level was negatively correlated with that of LINC00488. Rescue experiments showed that knockdown of miRNA-485-5p reversed the attenuated proliferation and migration of esophageal cancer cells with LINC00488-knockdown. In conclusion, LINC00488 aggravated the malignant progression of esophageal cancer by targeting miRNA-485-5p.

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“…including AC005104.1, AC093278.2, AC098484.1, AL360181.2, LINC01011, AP001372.2, AC007637.1, AL354733.3, and AP001189.3, which were the first time that served as markers in the current research. The lncRNA EMX2OS, which has been validated and identified to potentially induce proliferation and invasion, and promote sphere formation in ovarian cancer cells by regulating the miR-654-3p/AKT3/PD-L1 Axis 28 Additionally, EMX2OS can be used as a biomarker in laryngeal cancer and papillary thyroid cancer 29 , 30 . Furthermore, SPINT1-AS1 was identified as a potential marker in the prognosis of colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

Identification of 12 immune-related lncRNAs and molecular subtypes for the clear cell renal cell carcinoma based on RNA sequencing data

Zhong

Chen

Zhong

et al. 2020

Sci Rep

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Clear cell renal cell carcinoma (ccRCC) is the most common type of renal cell carcinoma (RCC). Despite the existing extensive research, the molecular and pathogenic mechanisms of ccRCC are elusive. We aimed to identify the immune-related lncRNA signature and molecular subtypes associated with ccRCC. By integrating 4 microarray datasets from Gene Expression Omnibus database, we identified 49 immune-related genes. The corresponding immune-related lncRNAs were further identified in the TCGA dataset. 12-lncRNAs prognostic and independent signature was identified through survival analysis and survival difference between risk groups was further identified based on the risk score. Besides, we identified 3 molecular subtypes and survival analysis result showed that cluster 2 has a better survival outcome. Further, ssGSEA enrichment analysis for the immune-associated gene sets revealed that cluster 1 corresponded to a high immune infiltration level. While cluster 2 and cluster 3 corresponded to low and medium immune infiltration level, respectively. In addition, we validated the 12-lncRNA prognostic signature and molecular subtypes in an external validation dataset from the ICGC database. In summary, we identified a 12-lncRNA prognostic signature which may provide new insights into the molecular mechanisms of ccRCC and the molecular subtypes provided a theoretical basis for personalized treatment by clinicians. Renal cell carcinoma (RCC) accounts for over 90% of all kidney cancer cases among human adults 1. Clear cell renal cell carcinoma (ccRCC) is the most common RCC histological subtype which responsible for 70-80% of RCC cases 2. Recent studies estimate that 102,000 patients succumb to ccRCC and 202,000 new cases are diagnosed annually throughout the globe 3. Besides, ccRCC has no clear symptoms in its early stages therefore can only be diagnosed in advanced stages 4. Despite interventions through chemotherapy and radiotherapy, ccRCC is prone to distant metastasis. These metastases occur in lung, bone, liver, distant lymph nodes, and renal vein 5. A few effective biomarkers, particularly in both early and advanced stages of ccRCC, have been identified. The recent version of the human genome annotation transcribes approximately 16,000 long non-coding RNAs (lncRNAs) responsible for 26.7% of the total genes (https ://www.genco degen es.org/human /) 6. lncRNAs are non-coding RNAs composed of about 200 nucleotides in length. They include the antisense lncRNAs, intronic transcripts, large intergenic noncoding RNAs (lincRNAs), promoter-associated lncRNAs, and UTR associated lncRNAs 7. Previous studies have revealed that lncRNA plays an important role in regulating gene expression, epigenetics, cell differentiation, and ontogeny 8,9. Additionally, studies have shown that massive aberrantly expressed

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Signature microRNAs and long noncoding RNAs in laryngeal cancer recurrence identified using a competing endogenous RNA network

Cited by 17 publications

References 62 publications

Liquid lncRNA Biopsy for the Evaluation of Locally Advanced and Metastatic Squamous Cell Carcinomas of the Head and Neck

Liquid lncRNA Biopsy for the Evaluation of Locally Advanced and Metastatic Squamous Cell Carcinomas of the Head and Neck

LINC00488 stimulates the progression of esophageal cancer by targeting microRNA‑485‑5p

Identification of 12 immune-related lncRNAs and molecular subtypes for the clear cell renal cell carcinoma based on RNA sequencing data

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