Signature-based analysis of MET proto-oncogene mutations using DHPLC

Pitfalls in the Denaturing High-Performance Liquid Chromatography Analysis of Mitochondrial DNA Mutation

“…Similarly, analysis of the melt profiles at 57°C to 61°C did not reveal any difference among these four mutations. Therefore, contrary to reports detailing DHPLC analysis of nuclear DNA mutations, [11][12][13][14] we observed no characteristic DHPLC signatures for four common mitochondrial DNA mutations. DNA sequencing is necessary for the identification of the mutation even when the profiles show very similar characteristic patterns.…”

Pitfalls in the Denaturing High-Performance Liquid Chromatography Analysis of Mitochondrial DNA Mutation

“…Similarly, analysis of the melt profiles at 57°C to 61°C did not reveal any difference among these four mutations. Therefore, contrary to reports detailing DHPLC analysis of nuclear DNA mutations, [11][12][13][14] we observed no characteristic DHPLC signatures for four common mitochondrial DNA mutations. DNA sequencing is necessary for the identification of the mutation even when the profiles show very similar characteristic patterns.…”

Site-Directed Mutagenesis of Exon 5 of p53 : Purification, Analysis, and Validation of Amplicons for DHPLC

“…The small proportion of the total peak area attributable to PCR-induced mutations indicates that the sequence fidelity of the PCR product is high. As noted by others (Nickerson et al, 2000), the pattern of peaks varies for different mutations in the same fragment at the same temperature (Fig. 7c-g).…”

Random mutagenesis-PCR to introduce alterations into defined DNA sequences for validation of SNP and mutation detection methods

“…This prediction is supported by the results presented here (Fig. 3) that allowed rapid identification of a sequence change from its DHPLC signature [Nickerson et al, 2000;Arnold et al, 1999;Gross et al, 1999].…”

“…Unique DHPLC signatures for each clone are presented in Figure 3, confirming the association between DHPLC elution profiles and specific sequence changes [Nickerson et al, 2000;Arnold et al, 1999;Gross et al, 1999]. Signatures were analyzed by shape and peak retention time(s).…”

Pitfalls in the Denaturing High-Performance Liquid Chromatography Analysis of Mitochondrial DNA Mutation

“…Similarly, analysis of the melt profiles at 57°C to 61°C did not reveal any difference among these four mutations. Therefore, contrary to reports detailing DHPLC analysis of nuclear DNA mutations, [11][12][13][14] we observed no characteristic DHPLC signatures for four common mitochondrial DNA mutations. DNA sequencing is necessary for the identification of the mutation even when the profiles show very similar characteristic patterns.…”

Site-Directed Mutagenesis of Exon 5 of p53 : Purification, Analysis, and Validation of Amplicons for DHPLC

“…The small proportion of the total peak area attributable to PCR-induced mutations indicates that the sequence fidelity of the PCR product is high. As noted by others (Nickerson et al, 2000), the pattern of peaks varies for different mutations in the same fragment at the same temperature (Fig. 7c-g).…”

Random mutagenesis-PCR to introduce alterations into defined DNA sequences for validation of SNP and mutation detection methods

“…This prediction is supported by the results presented here (Fig. 3) that allowed rapid identification of a sequence change from its DHPLC signature [Nickerson et al, 2000;Arnold et al, 1999;Gross et al, 1999].…”

“…Unique DHPLC signatures for each clone are presented in Figure 3, confirming the association between DHPLC elution profiles and specific sequence changes [Nickerson et al, 2000;Arnold et al, 1999;Gross et al, 1999]. Signatures were analyzed by shape and peak retention time(s).…”

Pitfalls in the Denaturing High-Performance Liquid Chromatography Analysis of Mitochondrial DNA Mutation

“…Similarly, analysis of the melt profiles at 57°C to 61°C did not reveal any difference among these four mutations. Therefore, contrary to reports detailing DHPLC analysis of nuclear DNA mutations, [11][12][13][14] we observed no characteristic DHPLC signatures for four common mitochondrial DNA mutations. DNA sequencing is necessary for the identification of the mutation even when the profiles show very similar characteristic patterns.…”

Site-Directed Mutagenesis of Exon 5 of p53 : Purification, Analysis, and Validation of Amplicons for DHPLC

“…The small proportion of the total peak area attributable to PCR-induced mutations indicates that the sequence fidelity of the PCR product is high. As noted by others (Nickerson et al, 2000), the pattern of peaks varies for different mutations in the same fragment at the same temperature (Fig. 7c-g).…”

Random mutagenesis-PCR to introduce alterations into defined DNA sequences for validation of SNP and mutation detection methods

“…This prediction is supported by the results presented here (Fig. 3) that allowed rapid identification of a sequence change from its DHPLC signature [Nickerson et al, 2000;Arnold et al, 1999;Gross et al, 1999].…”

“…Unique DHPLC signatures for each clone are presented in Figure 3, confirming the association between DHPLC elution profiles and specific sequence changes [Nickerson et al, 2000;Arnold et al, 1999;Gross et al, 1999]. Signatures were analyzed by shape and peak retention time(s).…”