Signalling Pathways Involved in Adult Heart Formation Revealed by Gene Expression Profiling in Drosophila

Zeitouni, Bruno; Senatore, Sébastien; Séverac, Dany; Aknin, Cindy; Sémériva, Michel; Perrin, Laurent

doi:10.1371/journal.pgen.0030174

Cited by 69 publications

(100 citation statements)

References 52 publications

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“…In adult Drosophila, the origin and nature of the 4 pairs of ostial valves are well documented in the literature (Rizki, 1978;Curtis et al, 1999;Bodmer et al, 2005;Zeitouni et al, 2007). Recently, Wasserthal (2007) reported finding of another pair of ostial apertures in the anterior part of the conical chamber.…”

Section: Discussionmentioning

confidence: 96%

“…He describes the formation of the anterior conical heart chamber during metamorphosis and predicts, quite prophetically, that the origin of the conical chamber might be responsible for reversal of the heartbeat. Currently there are more detailed anatomical descriptions of the dorsal vessel in Drosophila (Curtis et al, 1999;Dulcis & Levine, 2005;Wasserthal, 2007;Zeitouni et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Physiology of heartbeat reversal in adult Drosophila melanogaster (Diptera: Drosophilidae)

Sláma¹

2010

Eur. J. Entomol.

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Abstract. Heartbeat patterns were monitored in the living bodies of decapitated adult flies using several electrocardiographic methods (pulse-light optocardiography, thermocardiography, strain-gauge cardiography). Unlike other insect species, in which there is a peristaltic segmental propagation of cardiac contractions, Drosophila uses extremely efficient synchronic cardiac contractions. The rate of synchronic cardiac pulsation, which is characterized by simultaneous propagation of anterograde systolic contractions along all the segments of the heart, is relatively fast (~ 4 Hz at room temperature). This pulsation is used mainly for the vigorous pumping of haemolymph into the head and thorax through a narrow elastic tube, the aorta (anterograde I heartbeat). In addition, this synchronic pulsation is also used to enhance the circulation of haemolymph throughout the abdominal body cavity (anterograde II heartbeat). The switch between thoracic (anterograde I) and abdominal (anterograde II) haemolymph circulation is regulated by periodically alternating, tetanic contractions and relaxations of the conical heart chamber (ventricle). In the latter there is a pair of slitlike apertures, which are closed or opened by contraction or relaxation of the organ, respectively. During contraction of the conical chamber, the apertures are tightly constricted for several seconds and haemolymph is pumped forwards into the aorta (anterograde I heartbeat). Conversely, during relaxation of the conical chamber, the apertures are wide open for a few seconds, haemolymph leaves the heart and leaks out through open apertures and circulates from the tail to the base of the abdomen. The backward oriented, retrograde heartbeat recorded in other insects, has a lower frequency (1 to 2 Hz), occurs in Drosophila only sporadically and usually in the form of individual or twinned systolic peaks of large amplitude. Unlike the synchronic nature of the anterograde I and II cardiac contractions, propagation of the relatively slow retrograde heartbeat is by peristalsis. The newly discovered, compact ventricle with atrium and synchronic functioning of the insect heart shows structural and functional analogies with the functioning of the human heart.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Physiology of heartbeat reversal in adult Drosophila melanogaster (Diptera: Drosophilidae)

Sláma¹

2010

Eur. J. Entomol.

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“…We provide a concise account of the steps refined by and commonly employed in our lab for effective staining that permits well-resolved in situ imaging of larval and adult Drosophila heart tubes. Others have described similar methods in abbreviated form 2,3,4 .…”

Section: Discussionmentioning

confidence: 99%

Fluorescent Labeling of <em>Drosophila</em> Heart Structures

Alayari

Vogler

Taghli-Lamallem

et al. 2009

JoVE

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The Drosophila melanogaster dorsal vessel, or heart, is a tubular structure comprised of a single layer of contractile cardiomyocytes, pericardial cells that align along each side of the heart wall, supportive alary muscles and, in adults, a layer of ventral longitudinal muscle cells. The contractile fibers house conserved constituents of the muscle cytoarchitecture including densely packed bundles of myofibrils and cytoskeletal/submembranous protein complexes, which interact with homologous components of the extracellular matrix. Here we describe a protocol for the fixation and the fluorescent labeling of particular myocardial elements from the hearts of dissected larvae and semi-intact adult Drosophila. Specifically, we demonstrate the labeling of sarcomeric F-actin and of α-actinin in larval hearts. Additionally, we perform labeling of F-actin and α-actinin in myosin-GFP expressing adult flies and of α-actinin and pericardin, a type IV extracellular matrix collagen, in wild type adult hearts. Particular attention is given to a mounting strategy for semi-intact adult hearts that minimizes handling and optimizes the opportunity for maintaining the integrity of the cardiac tubes and the associated tissues. These preparations are suitable for imaging via fluorescent and confocal microscopy. Overall, this procedure allows for careful and detailed analysis of the structural characteristics of the heart from a powerful genetically tractable model system.

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“…de signalisation dont certaines, à l'image de la voie PVF (pour PDGF [platelet-derived growth factor]/ VEGF [vascular endothelial growth factor]) qui est impliquée dans la différenciation des valves au nombre de trois dans l'organe adulte [6], ont un rôle similaire chez les mammifères. Tout comme chez l'embryon et la larve, le muscle cardiaque adulte est constitué d'une simple couche de myocytes dont les fibres sont orientées de manière transverse par rapport à l'axe du tube.…”

Section: Synthèse Revuesunclassified