Signalling networks regulating cyclooxygenase-2

Tsatsanis, Christos; Androulidaki, Ariadne; Venihaki, Maria; Margioris, Andrew N.

doi:10.1016/j.biocel.2006.03.021

Cited by 446 publications

(400 citation statements)

References 55 publications

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“…We also observed higher constitutive levels of COX-2 in mDC from HIV-1 infected individuals. COX-2 is up-regulated by TLR agonists such as CpG ODN and LPS in a MyD88-dependent signaling pathway (22)(23)(24). In these studies we demonstrate direct modulation of COX-2 expression in mDC using TLR4, TLR7/8, and TLR8 agonists.…”

Section: Discussionmentioning

confidence: 50%

“…COX-2 expression is regulated through the MyD88 dependent pathway (22,23) and is induced by pathogens, microbes and synthetic TLR agonists including CpG DNA (TLR9) and lipopolysaccharides (LPS, TLR4) (24). Continuous antigenic stimulation and inflammation observed during chronic infections, including HIV-1, may inhibit effector T cell function through COX-2 expression and the production of prostaglandins (25).…”

Section: Introductionmentioning

confidence: 99%

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Dendritic cells from HIV-1 infected individuals are less responsive to toll-like receptor (TLR) ligands

Martinson

Román-Gonzaléz

Tenorio

et al. 2007

Cellular Immunology

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We compared TLR responsiveness in PBMC from HIV-1-infected and uninfected individuals using the TLR agonists: TLR7 (3M-001), TLR8 (3M-002) and TLR7/8 (3M-011). Activation and maturation of plasmacytoid dendritic cells (pDC)were measured by evaluating CD86, CD40 and CD83 expression and myeloid dendritic cell (mDC) activation was measured by evaluating CD40 expression. All agonists tested induced activation and maturation of pDC in PBMC cultures of cells from HIV+ and HIV− individuals. The TLR7 agonist induced significantly less pDC maturation in cells from HIV+ individuals. Quantitative assessment of secreted IFN-α and pro-inflammatory cytokines at the single cell level showed that pDC from HIV+ individuals stimulated with TLR7 and TLR7/8 induced IFN-α. TLR8 and TLR7/8 agonists induced IL-12 and COX-2 expression in mDC from HIV+ and HIV− individuals. Understanding pDC and mDC activation and maturation in HIV-1 infection could lead to more rational development of immunotherapeutic strategies to stimulate the adaptive immune response to HIV-1.

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Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Dendritic cells from HIV-1 infected individuals are less responsive to toll-like receptor (TLR) ligands

Martinson

Román-Gonzaléz

Tenorio

et al. 2007

Cellular Immunology

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“…Different pathways control the expression of iNOS or COX-2. Whereas the main regulatory step for the iNOS is the activation of the transcription factor NF-jB [28], additional factors regulate the gene expression for COX-2 [31]. Moreover, COX-2 is also affected posttranscriptionally at the level of mRNA stability, and its activity is known to be affected by NO [31].…”

Section: Discussionmentioning

confidence: 99%

Polymeric proanthocyanidins from Sicilian pistachio (Pistacia vera L.) nut extract inhibit lipopolysaccharide-induced inflammatory response in RAW 264.7 cells

et al. 2011

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Background Positive effects of pistachio nut consumption on plasma inflammatory biomarkers have been described; however, little is known about molecular events associated with these effects. Purpose We studied the anti-inflammatory activity of a hydrophilic extract from Sicilian Pistacia L. (HPE) in a macrophage model and investigated bioactive components relevant to the observed effects. Methods HPE oligomer/polymer proanthocyanidin fractions were isolated by adsorbance chromatography, and components quantified as anthocyanidins after acidic hydrolysis. Isoflavones were measured by gradient elution HPLC analysis. RAW 264.7 murine macrophages were pre-incubated with either HPE (1-to 20-mg fresh nut equivalents) or its isolated components for 1 h, then washed before stimulating with lipopolysaccharide (LPS) for 24 h. Cell viability and parameters associated with Nuclear Factor-jB (NF-jB) activation were assayed according to established methods including ELISA, Western blot, or cytofluorimetric analysis. Results HPE suppressed nitric oxide (NO) and tumor necrosis factor-a (TNF-a) production and inducible NO-synthase levels dose dependently, whereas inhibited prostaglandin E 2 (PGE 2 ) release and decreased cyclooxygenase-2 content, the lower the HPE amount the higher the effect. Cytotoxic effects were not observed. HPE also caused a dose-dependent decrease in intracellular reactive oxygen species and interfered with the NF-jB activation.Polymeric proanthocyanidins, but not isoflavones, at a concentration comparable with their content in HPE, inhibited NO, PGE 2 , and TNF-a formation, as well as activation of IjB-a. Oligomeric proanthocyanidins showed only minor effects. Conclusions Our results provide molecular evidence of anti-inflammatory activity of pistachio nut and indicate polymeric proanthocyanidins as the bioactive components. The mechanism may involve the redox-sensitive transcription factor NF-jB. Potential effects associated with pistachio nut consumption are discussed in terms of the proanthocyanidin bioavailability.

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“…Among these pathways, mitogen-activated proteins kinases (MAPKs) are signaling molecules that play critical roles in the regulation of cell growth, differentiation, cell survival/apoptosis and cellular response to cytokines and stress. The MAPKs pathways include p38 MAPK (Han et al, 1994), c-jun NH 2 -terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) (Davis, 1994), and they are involved on LPS-induced COX-2 and iNOS expression in macrophages Chen and Wang, 1999;Tsatsanis et al, 2006). Accordingly, it has been demonstrated that MAPKs inhibitors suppress the expression of iNOS gene .…”

Section: Introductionmentioning

confidence: 99%

Cymbopogon citratus as source of new and safe anti-inflammatory drugs: Bio-guided assay using lipopolysaccharide-stimulated macrophages

Francisco

Figueirinha

Neves

et al. 2011

Journal of Ethnopharmacology

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Please cite this article as: Francisco, V., Figueirinha, A., Neves, B.M., García-Rodríguez, C., Lopes, M.C., Cruz, M.T., Batista, M.T., Cymbopogon citratus as source of new and safe anti-inflammatory drugs: bio-guided assay using lipopolysaccharide-stimulated macrophages, Journal of Ethnopharmacology (2010Ethnopharmacology ( ), doi:10.1016Ethnopharmacology ( /j.jep.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 30A c c e p t e d M a n u s c r i p t 1 Graphical abstractIn this report it was demonstrated that a lipid-and essential oil-free infusion of Cymbopogon citratus leaves (Cy), as well its polyphenols, have anti-inflammatory properties through inhibition of proinflammatory signaling pathways and nitric oxide production in lipopolysaccharide-stimulated macrophages. These evidences support the use of Cymbopogon citratus in traditional medicine and indicate that it could be a natural source of new and safe anti-inflammatory drugs. TitleCymbopogon citratus as source of new and safe anti-inflammatory drugs: bio-guided assay using lipopolysaccharide-stimulated macrophages Aim of the study: The aim of this study is to explore the anti-inflammatory properties of Cymbopogon citratus leaves and their polyphenol-rich fractions (PFs), as well its mechanism of action in murine macrophages. Materials and Methods:A lipid-and essential oil-free infusion of Cy leaves was prepared (Cy extract) and fractionated by column chromatography. Anti-inflammatory properties of Cy extract (1.115 mg/ml) and its PFs, namely phenolic acids (530 µg/ml), flavonoids (97.5 µg/ml) and tannins (78 µg/ml), were investigated using lipopolysaccharide (LPS)-stimulated Raw 264.7 macrophages as in vitro model. As inflammatory parameters, nitric oxide (NO) production was evaluated by Griess reaction, as well as effects on cyclooxygenase (COX-2), inducible NO synthase (iNOS) expression and on intracellular signaling pathways activation, which were analyzed by Western blot using specific antibodies. Page 3 of 30A c c e p t e d M a n u s c r i p t Conclusions: Our data provide evidence that support the usage of Cymbopogon citratus leaves extract in traditional medicine, and suggest that Cy, in particular its polyphenolic compounds, could constitute a natural source of a new and safe anti-inflammatory drugs.

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Signalling networks regulating cyclooxygenase-2

Cited by 446 publications

References 55 publications

Dendritic cells from HIV-1 infected individuals are less responsive to toll-like receptor (TLR) ligands

Dendritic cells from HIV-1 infected individuals are less responsive to toll-like receptor (TLR) ligands

Polymeric proanthocyanidins from Sicilian pistachio (Pistacia vera L.) nut extract inhibit lipopolysaccharide-induced inflammatory response in RAW 264.7 cells

Cymbopogon citratus as source of new and safe anti-inflammatory drugs: Bio-guided assay using lipopolysaccharide-stimulated macrophages

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