Signaling by Cellular Protrusions: Keeping the Conversation Private

Buszczak, Michael; Inaba, Mayu; Yamashita, Yukiko

doi:10.1016/j.tcb.2016.03.003

Cited by 62 publications

(65 citation statements)

References 60 publications

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“…Cellular enwrapment of hematopoietic stem cells by the perivascular niche of zebrafish has been referred to as “endothelial cuddling” (Tamplin et al, 2015). It has been speculated that enwrapment of stem cells might facilitate stem cell-niche signaling (Buszczak et al, 2016; Kornberg and Roy, 2014) or adhesion (S. Chen et al, 2013) to promote stem cell fate. Direct evidence for a role for enwrapment of stem cells, however, is lacking.…”

Section: Introductionmentioning

confidence: 99%

Identification of regulators of germ stem cell enwrapment by its niche in C. elegans

Linden

Gordon

Pani

et al. 2017

Developmental Biology

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Many stem cell niches contain support cells that increase contact with stem cells by enwrapping them in cellular processes. One example is the germ stem cell niche in C. elegans, which is composed of a single niche cell termed the distal tip cell (DTC) that extends cellular processes, constructing an elaborate plexus that enwraps germ stem cells. To identify genes required for plexus formation and to explore the function of this specialized enwrapping behavior, a series of targeted and tissue-specific RNAi screens were performed. Here we identify genes that promote stem cell enwrapment by the DTC plexus, including a set that specifically functions within the DTC, such as the chromatin modifier lin-40/MTA1, and others that act within the germline, such as the 14-3-3 signaling protein par-5. Analysis of genes that function within the germline to mediate plexus development reveal that they are required for expansion of the germ progenitor zone, supporting the emerging idea that germ stem cells signal to the niche to stimulate enwrapping behavior. Examination of wild-type animals with asymmetric plexus formation and animals with reduced DTC plexus elaboration via loss of two candidates including lin-40 indicate that cellular enwrapment promotes GLP-1/Notch signaling and germ stem cell fate. Together, our work identifies novel regulators of cellular enwrapment and suggests that reciprocal signaling between the DTC niche and the germ stem cells promotes enwrapment behavior and stem cell fate.

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Section: Introductionmentioning

confidence: 99%

Identification of regulators of germ stem cell enwrapment by its niche in C. elegans

Linden

Gordon

Pani

et al. 2017

Developmental Biology

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“…Recent evidence however suggests the distance over which contact mediated signaling of various types can operate can be extended by cellular protrusions capable of spanning several cell diameters (reviewed in Buszczak et al, 2016, Prols et al, 2016. For example, signaling through long cellular protrusions plays a role during limb patterning in the chick embryo (Sanders et al, 2013), in the development of the zebrafish pigmentation stripes (Eom et al, 2015) as well as neural plate patterning also in the zebrafish (Stanganello et al 2015).…”

mentioning

confidence: 99%

Basal protrusions mediate spatiotemporal patterns of spinal neuron differentiation

Hadjivasiliou

Moore

McIntosh

et al. 2019

Preprint

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During early spinal cord development, neurons of particular subtypes differentiate with a sparse periodic pattern while later neurons differentiate in the intervening space to eventually produce continuous columns of similar neurons. The mechanisms that regulate this spatiotemporal pattern are unknown. In vivo imaging of zebrafish reveals differentiating spinal neurons transiently extend two long protrusions along the basal surface of the spinal cord prior to axon initiation. These protrusions express Delta protein consistent with the possibility they influence Notch signalling at a distance of several cell diameters. Experimental reduction of laminin expression leads to smaller protrusions and shorter distances between differentiating neurons. The experimental data and a theoretical model support the proposal that the pattern of neuronal differentiation is regulated by transient basal protrusions that deliver temporally controlled lateral inhibition mediated at a distance. This work uncovers novel, stereotyped protrusive activity of new-born neurons that organizes long distance spatiotemporal patterning of differentiation.

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“…Since the printing of cells, and their transfer to a number of different substrates such as polystyrene or glass, is a controllable characteristic in this device, and because studies of signal transduction between primary neurons require their precise individual positioning, the utilization of this device to print individual cortical neurons was also used after to obtain single and paired neurons with highly branched dendrites. The use of protrusion enhances the specificity for cells to communicate between each other 121 . Chemical cell-cell communication is not the only way in which cells use protrusions in nature.…”

Section: Long Distance Communicationmentioning

confidence: 99%

“…In fact, cells can use protrusion for several different biological roles that range from expansion of their surface area to cell motility and more 122 . Therefore, the use of microfluidics devices for these studies provides new and important tools to help better understand the role of cell protrusions in several cellular processes, specifically cellular signaling and communication 121 .…”

Section: Long Distance Communicationmentioning

confidence: 99%

Bridging the gap: microfluidic devices for short and long distance cell–cell communication

Castro

Qin

2017

Lab Chip

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Cell-cell communication is a crucial component of many biological functions. For example, understanding how immune cells and cancer cells interact, both at the immunological synapse and through cytokine secretion, can help us understand and improve cancer immunotherapy. The study of how cells communicate and form synaptic connections is important in neuroscience, ophthalmology, and cancer. But in order to increase our understanding of these cellular phenomena, better tools need to be developed that allow us to study cell-cell communication in a highly controlled manner. Some technical requirements for better communication studies include manipulating cells spatiotemporally, high resolution imaging, and integrating sensors. Microfluidics is a powerful platform that has the ability to address these requirements and other current limitations. In this review, we describe some new advances in microfluidic technologies that have provided researchers with novel methods to study intercellular communication. The advantages of microfluidics have allowed for new capabilities in both single cell-cell communication and population-based communication. This review highlights microfluidic communication devices categorized as “short distance,” or primarily at the single cell level, and “long distance,” which mostly encompasses population level studies. Future directions and translation/commercialization will also be discussed.

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Signaling by Cellular Protrusions: Keeping the Conversation Private

Cited by 62 publications

References 60 publications

Identification of regulators of germ stem cell enwrapment by its niche in C. elegans

Identification of regulators of germ stem cell enwrapment by its niche in C. elegans

Basal protrusions mediate spatiotemporal patterns of spinal neuron differentiation

Bridging the gap: microfluidic devices for short and long distance cell–cell communication

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