sIgD- and sIgD+ B cells responding to thymus-independent antigens have a different lymphokine requirement.

Waldschmidt, Thomas J.; Layton, Judith E.; Mcfadden, S F; Uhr, J W; Vitetta, Ellen S.

doi:10.4049/jimmunol.135.2.865

Cited by 4 publications

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Comparison of the clonal diversity of the B cell repertoires in adult mice that differ in the expression of cell surface IgD

1987

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To determine whether the expression of surface IgD (sIgD) influences the extent of the expressed B cell repertoire, the clonal diversity of the B cell population in mice treated chronically with anti-IgD (delta) antibodies has been compared with the B cell repertoire observed in control animals, using the splenic focus limiting dilution B cell assay. The results show that the phosphorylcholine (PC)-specific B cell precursor frequency in anti-delta antibody-treated mice is increased when compared with that of control mice. Isotype and idiotype (T15) analyses of PC clonal products from anti-delta antibody-treated and control mice revealed no distributional differences. Analyses of the 2,4-dinitrophenyl (DNP)- and fluoresein isothiocyanate-specific B cell repertoires confirmed that the equal or increased precursor frequencies observed in anti-delta antibody-treated mice are not specific for the PC antigen. The increased precursor frequency of B cells from anti-delta antibody-treated mice was not the result of increased homing of B cells from anti-delta antibody-treated mice to recipient spleens, since B cells from control mice homed twice as well to recipient spleens as did B cells from anti-delta antibody-treated mice. Other studies demonstrated that (a) on average, antibody-secreting clones were generated more slowly when B cells from anti-delta antibody-treated mice were used as a source of precursors than B cells of control mice and (b) both sIg- spleen cells and sIg+ spleen cells from anti-delta antibody-treated mice generated a higher frequency of specific antibody-secreting clones than did the corresponding populations from control mice. These observations suggest that a population of sIgM+sIgD- B cells exists that resembles sIgD+ B cells rather than neonatal or xid B cells in its ability to generate responses to PC and suggests that the sIgM+sIgD- B cells from anti-delta antibody-treated mice are more responsive than are sIgM+IgD+ B cells, regardless of antigenic specificity, to the stimuli provided in the splenic focus system. Finally, this study suggests that the expression of sIgD does not influence the extent of the expressed B cell repertoire.

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Comparison of the clonal diversity of the B cell repertoires in adult mice that differ in the expression of cell surface IgD

1987

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Regulation of Human B Lymphocyte Activation, Proliferation, and Differentiation

Jelinek

Lipsky

1987

Advances in Immunology

143

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Independent regulation of IgM, IgD, and Ia antigen expression in cultured immature B lymphocytes.

Dasch¹,

Jones²

1986

The Journal of Experimental Medicine

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Long-term cultured bone marrow cells were characterized with respect to a number of B and pre-B cell markers. Cells expressing ThB, B-220, and IgM were found within cultures set up according to the procedure of Whitlock and Witte. This culture system was modified by placing sorted pre-B cells (ThB+, IgM-) from bone marrow in culture with previously-established bone marrow adherent layers. These cultures commenced growth without the lag associated with the Whitlock cultures. These cultured nonadherent cells show a high frequency of IgM+ cells, but do not express either IgD or Ia, and we refer to them as immature B cells. Cells with a similar phenotype (IgM+, Ia-, IgD-) are found within the spleens of young but not adult mice. The phorbol ester PMA induces expression of IgD on the cultured immature B cells, but has no effect on Ia expression. This suggests that the processing of H chain RNA transcripts may be affected by protein kinase C. These results demonstrate that the appearance of IgM, IgD, and Ia are independently controlled in long-term cultured B-lineage cells.

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sIgD- and sIgD+ B cells responding to thymus-independent antigens have a different lymphokine requirement.

Cited by 4 publications

References 0 publications

Comparison of the clonal diversity of the B cell repertoires in adult mice that differ in the expression of cell surface IgD

Comparison of the clonal diversity of the B cell repertoires in adult mice that differ in the expression of cell surface IgD

Regulation of Human B Lymphocyte Activation, Proliferation, and Differentiation

Independent regulation of IgM, IgD, and Ia antigen expression in cultured immature B lymphocytes.

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