Shrinkage and colour in the production of micro-sized PDMS particles for microfluidic applications

Anes, C. F.; Pinho, Diana; Muñoz-Sánchez, B.N.; Vega, E. J.; Lima, Rui

doi:10.1088/1361-6439/aab7b9

Cited by 14 publications

(17 citation statements)

References 26 publications

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“…The procedure, experimental setup and flow-focusing technique used in this study to generate the monodisperse PDMS microparticles were well explained and described in [7] and [48] .…”

Section: Microparticles Production Proceduresmentioning

confidence: 99%

“…Three different samples were prepared with the transparent PDMS, by varying the mixture proportions as 10:1, 8:2 and 6:4. Also, based on the work performed by Anes et al [48] , the transparent PDMS mixed at a ratio of 10:1 was dyed with a red pigment for silicone (paste of PP SIL RO-1, Plastiform) at 20 wt%, in order to produce coloured microparticles.…”

Section: Microparticles Production Proceduresmentioning

confidence: 99%

“…Clearly, DI measurements for the pigmented samples are far from the human RBCs values, either the healthy or the pathological ones. Hence, even if these coloured particles enhance contrast and visualization [48] , they do not seem to be adequate to represent RBCs in the development of particulate analogue fluids.…”

Section: Deformation Index (Di)mentioning

confidence: 99%

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Flexible PDMS microparticles to mimic RBCs in blood particulate analogue fluids

Pinho

Muñoz-Sánchez

Anes

et al. 2019

Mechanics Research Communications

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Polydimethylsiloxane (PDMS) has a wide variety of commercial and industrial applications due to its mechanical and rheological properties in a range similar to the living tissues. In this study, we demonstrate that PDMS can be used to produce deformable microparticles to be integrated in the development of particulate blood analogue fluids. The difficulties associated with the use of in vitro blood make it necessary to perform in vitro experiments of blood flow with blood analogue fluids. However, an ideal analogue must match the rheology of blood at several points, and for that, blood analogue fluids should be a suspension of microparticles with similar properties (size, shape and flexibility) to blood cells, in particular to the red blood cells (RBCs). The microparticles used in this study were produced from a transparent PDMS with crosslinking ratios of 10:1, 8:2 and 6:4; from a black PDMS with a ratio of 1:1 and from a red-pigmented PDMS. Each PDMS microparticles sample was suspended in Dextran 40 to perform deformability assays and cell-free layer analysis in a hyperbolic-shaped microchannel and steady shear viscosity measurements in a rheometer. The proposed microparticles suspensions show a great potential to mimic the structural and rheological properties of RBC suspensions and consequently to develop blood analogue fluids with rheological properties similar to real blood.

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“…The procedure, experimental setup and flow-focusing technique used in this study to generate the monodisperse PDMS microparticles were well explained and described in [7] and [48] .…”

Section: Microparticles Production Proceduresmentioning

confidence: 99%

Section: Microparticles Production Proceduresmentioning

confidence: 99%

See 1 more Smart Citation

Flexible PDMS microparticles to mimic RBCs in blood particulate analogue fluids

Pinho

Muñoz-Sánchez

Anes

et al. 2019

Mechanics Research Communications

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“…In addition, the droplet-based microfluidics can provide encapsulation of a single cell within a droplet, which allows for high-throughput single cell screening and analysis capabilities [5,6]. The droplet-based microfluidic systems have been successfully utilized in a variety of applications, such as single cell analysis/screening [7][8][9], protein crystallization [10], polymer chain reaction (PCR) [11], drug discovery [12][13][14], and microorganism reactions [15,16] to name a few. For all of these applications, producing droplets with controlled sizes as well as at controlled generation rates are key factors to obtain consistent and robust results.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator

et al. 2019

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There have been growing interests in droplet-based microfluidics due to its capability to outperform conventional biological assays by providing various advantages, such as precise handling of liquid/cell samples, fast reaction time, and extremely high-throughput analysis/screening. The droplet-based microfluidics utilizes the interaction between the interfacial tension and the fluidic shear force to break continuous fluids into uniform-sized segments within a microchannel. In this paper, the effect of different viscosities of carrier oil on water-in-oil emulsion, particularly how droplet size and droplet generation rate are affected, has been investigated using a commonly used T-junction microfluidic droplet generator design connected to a pressure-controlled pump. We have tested mineral oils with four different viscosities (5, 7, 10, and 15 cSt) to compare the droplet generation under five different flow pressure conditions (i.e., water flow pressure of 30–150 mbar and oil flow pressure of 40–200 mbar). The results showed that regardless of the flow pressure levels, the droplet size decreased as the oil viscosity increased. Average size of the droplets decreased by approximately 32% when the viscosity of the oil changed from 5 to 15 cSt at the flow pressure of 30 mbar for water and 40 mbar for oil. Interestingly, a similar trend was observed in the droplet generation rate. Droplet generation rate and the oil viscosity showed high linear correlation (R2 = 0.9979) at the water flow pressure 30 mbar and oil flow pressure 40 mbar.

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“…Size distribution of the micro carrier is an important factor governing the controlled release of functional materials [4]. Additionally, monodispersed microspheres have potential value for use as particulate blood analogues [5][6][7]. They have been mainly used as microfluidic systems to prepare monodispersed microspheres.…”

Section: Introductionmentioning

confidence: 99%

512-Channel Geometric Droplet-Splitting Microfluidic Device by Injection of Premixed Emulsion for Microsphere Production

2020

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We present a 512-channel geometric droplet-splitting microfluidic device that involves the injection of a premixed emulsion for microsphere production. The presented microfluidic device was fabricated using conventional photolithography and polydimethylsiloxane casting. The fabricated microfluidic device consisted of 512 channels with 256 T-junctions in the last branch. Five hundred and twelve microdroplets with a narrow size distribution were produced from a single liquid droplet. The diameter and size distribution of prepared micro water droplets were 35.29 µm and 8.8% at 10 mL/h, respectively. Moreover, we attempted to prepare biocompatible microspheres for demonstrating the presented approach. The diameter and size distribution of the prepared poly (lactic-co-glycolic acid) microspheres were 6.56 µm and 8.66% at 10 mL/h, respectively. To improve the monodispersity of the microspheres, we designed an additional post array part in the 512-channel geometric droplet-splitting microfluidic device. The monodispersity of the microdroplets prepared with the microfluidic device combined with the post array part exhibited a significant improvement.

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Shrinkage and colour in the production of micro-sized PDMS particles for microfluidic applications

Cited by 14 publications

References 26 publications

Flexible PDMS microparticles to mimic RBCs in blood particulate analogue fluids

Flexible PDMS microparticles to mimic RBCs in blood particulate analogue fluids

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator

512-Channel Geometric Droplet-Splitting Microfluidic Device by Injection of Premixed Emulsion for Microsphere Production

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