Shortwave Infrared in Vivo Imaging with Gold Nanoclusters

Chen, Yue; Montana, Daniel M.; He, Wei; Cordero, José Manuel Gonzalo; Schneider, Marc; Guével, Xavier Le; Ou, Changrong; Bruns, Oliver T.; Bawendi, Moungi G.

doi:10.1021/acs.nanolett.7b03070

Cited by 159 publications

(167 citation statements)

References 41 publications

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“…Increasing the quantum yield, functionality, and biocompatibility of SWIR fluorophores is an active focus of emerging research studies (24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41).…”

Section: Significancementioning

confidence: 99%

Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green

Carr

Franke

Caram

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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566

569

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Fluorescence imaging is a method of real-time molecular tracking in vivo that has enabled many clinical technologies. Imaging in the shortwave IR (SWIR; 1,000–2,000 nm) promises higher contrast, sensitivity, and penetration depths compared with conventional visible and near-IR (NIR) fluorescence imaging. However, adoption of SWIR imaging in clinical settings has been limited, partially due to the absence of US Food and Drug Administration (FDA)-approved fluorophores with peak emission in the SWIR. Here, we show that commercially available NIR dyes, including the FDA-approved contrast agent indocyanine green (ICG), exhibit optical properties suitable for in vivo SWIR fluorescence imaging. Even though their emission spectra peak in the NIR, these dyes outperform commercial SWIR fluorophores and can be imaged in the SWIR, even beyond 1,500 nm. We show real-time fluorescence imaging using ICG at clinically relevant doses, including intravital microscopy, noninvasive imaging in blood and lymph vessels, and imaging of hepatobiliary clearance, and show increased contrast compared with NIR fluorescence imaging. Furthermore, we show tumor-targeted SWIR imaging with IRDye 800CW-labeled trastuzumab, an NIR dye being tested in multiple clinical trials. Our findings suggest that high-contrast SWIR fluorescence imaging can be implemented alongside existing imaging modalities by switching the detection of conventional NIR fluorescence systems from silicon-based NIR cameras to emerging indium gallium arsenide-based SWIR cameras. Using ICG in particular opens the possibility of translating SWIR fluorescence imaging to human clinical applications. Indeed, our findings suggest that emerging SWIR-fluorescent in vivo contrast agents should be benchmarked against the SWIR emission of ICG in blood.

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Section: Significancementioning

confidence: 99%

Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green

Carr

Franke

Caram

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

566

569

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“…[20][21][22] Despite this potential to provide a high sensitivity and simple readout for early disease detection, to date AuNCs have been used in vivo solely for fluorescence and X-ray contrast bioimaging applications. [23][24][25] We therefore sought to leverage the catalytic activity of AuNCs to develop a nanosensor platform that produces a direct colorimetric readout of disease state.…”

Section: Mainmentioning

confidence: 99%

Renal clearable catalytic gold nanoclusters for in vivo disease monitoring

et al. 2019

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Ultra-small gold nanoclusters (AuNCs) have emerged as agile probes for in vivo imaging, as they exhibit exceptional tumour accumulation and efficient renal clearance properties.However, their intrinsic catalytic activity, which can enable increased detection sensitivity, has yet to be explored for in vivo sensing. By exploiting the peroxidase-mimicking activity of AuNCs and the precise nanometer size filtration of the kidney, we designed multifunctional protease nanosensors that respond to disease microenvironments to produce a direct colorimetric urinary readout of disease state in less than 1 h. We monitored the catalytic activity of AuNCs in collected urine of a mouse model of colorectal cancer where tumour-bearing mice showed a 13-fold increase in colorimetric signal compared to healthy mice. Nanosensors were eliminated completely through hepatic and renal excretion within 4 weeks after injection with no evidence of toxicity. We envision that this modular approach will enable rapid detection of a diverse range of diseases by exploiting their specific enzymatic signatures.

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“…These advantages have made NIR-II fluorescence bioimaging a method of choice for functional applications in mice, including whole-body angiography, organ visualization, as well as diagnosis and imaging-guided treatment of tumours [10][11][12][14][15][16][17][18][19][20][21][22] .…”

Section: Introductionmentioning

confidence: 99%

NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates

Cai

Zhu

Wang

et al. 2019

Preprint

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Vasculature architecture in the brain can provide revealing information about mental and neurological function and disease. Fluorescence imaging in the second near-infrared (NIR-II) regime with less light scattering is a more promising method for detecting cortical vessels than traditional visible and NIR-I modes. Here, for the first time, we developed, NIR-II fluorescence microscopy capabilities for imaging brain vasculature in macaque monkey. The first is a wide-field microscope with high temporal resolution (25 frames/second) for measuring blood flow velocity and cardiac impulse period, and the second is a high spatial resolution (<10 μm) confocal microscope producing three-dimensional maps of the cortical microvascular network (~500 μm deep). Both were designed with flexibility to image various cortical locations on the head. Use of a clinically approved dye provided high brightness in NIR-II region. This comprises an important advance towards studies of neurovascular coupling, stroke, and other diseases relevant to neurovascular health in humans.

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Shortwave Infrared in Vivo Imaging with Gold Nanoclusters

Cited by 159 publications

References 41 publications

Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green

Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green

Renal clearable catalytic gold nanoclusters for in vivo disease monitoring

NIR-II fluorescence microscopic imaging of cortical vasculature in non-human primates

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