Short-term osmotic responses of cells and tissues of the sea anemone, Condylactis gigantea

In vivo pH measurement at the site of calcification in an octocoral

“…The suspension of SNARF-1 AM-loaded cells was then transferred into a POC cell cultivation chamber (PECON) and cells were allowed to settle on the glass coverslip. After allowing 20 min for cells to load with SNARF-1 AM, the solution overlying the cells was removed by pipetting and replaced with calibration solutions 61 , 62 (Supplementary Material) ranging from pH 6 to 8.5.…”

In vivo pH measurement at the site of calcification in an octocoral

“…The suspension of SNARF-1 AM-loaded cells was then transferred into a POC cell cultivation chamber (PECON) and cells were allowed to settle on the glass coverslip. After allowing 20 min for cells to load with SNARF-1 AM, the solution overlying the cells was removed by pipetting and replaced with calibration solutions 61 , 62 (Supplementary Material) ranging from pH 6 to 8.5.…”

On the use of 31P NMR for the quantification of hydrosoluble phosphorus-containing compounds in coral host tissues and cultured zooxanthellae

“…All CZ used for the NMR measurements were collected at the same time of day (between 09:00 and 10:00 hrs), and were washed 3 times (centrifugation at 2000 g , for 5 min, at 4 °C) in a buffer (called the “cytoplasmic buffer”) 52 . This buffer contained approximate cytosolic concentrations of major ions in cnidarian cells 53 54 . Phosphatase inhibitors were added to prevent the hydrolysis of organic phosphorus compounds (50 mM NaF, 1 mM Na 3 VO 4 ) and mannitol to adjust osmolarity to 1,100 mOsm l −1 55 .…”

A Functional Biology of Sea Anemones

“…The concentration of mitochondria in the tentacles of A.elegantissima is twice as great as in the anemone as a whole (Table 3.1), and the volume density of mitochondria in tentacular endodermal cells of Aiptasia pulchella is about 3% of cell volume (J.P.S. These relationships may be owing in part to the proportionally smaller mass of essentially acellular mesoglea in the tentacles than in the rest of the anemone, as indicated by a larger extracellular (inulin) space in the body wall than in the tentacles (Herrera et al, 1989), as well as to a greater abundance of gland cells (which have relatively few mitochondria) in other than tentacular tissues. The mitochondrial concentration is also lower in the body wall of M.senile (1.56 X lOlO mitochondria/gwW) than in the entire anemone (4.02 x 1010 mitochondria/gwW), which implies a higher concentration of mitochondria in the tentacles (R.L.…”

In vivo pH measurement at the site of calcification in an octocoral

“…The suspension of SNARF-1 AM-loaded cells was then transferred into a POC cell cultivation chamber (PECON) and cells were allowed to settle on the glass coverslip. After allowing 20 min for cells to load with SNARF-1 AM, the solution overlying the cells was removed by pipetting and replaced with calibration solutions 61 , 62 (Supplementary Material) ranging from pH 6 to 8.5.…”

On the use of 31P NMR for the quantification of hydrosoluble phosphorus-containing compounds in coral host tissues and cultured zooxanthellae

“…All CZ used for the NMR measurements were collected at the same time of day (between 09:00 and 10:00 hrs), and were washed 3 times (centrifugation at 2000 g , for 5 min, at 4 °C) in a buffer (called the “cytoplasmic buffer”) 52 . This buffer contained approximate cytosolic concentrations of major ions in cnidarian cells 53 54 . Phosphatase inhibitors were added to prevent the hydrolysis of organic phosphorus compounds (50 mM NaF, 1 mM Na 3 VO 4 ) and mannitol to adjust osmolarity to 1,100 mOsm l −1 55 .…”

A Functional Biology of Sea Anemones

“…The concentration of mitochondria in the tentacles of A.elegantissima is twice as great as in the anemone as a whole (Table 3.1), and the volume density of mitochondria in tentacular endodermal cells of Aiptasia pulchella is about 3% of cell volume (J.P.S. These relationships may be owing in part to the proportionally smaller mass of essentially acellular mesoglea in the tentacles than in the rest of the anemone, as indicated by a larger extracellular (inulin) space in the body wall than in the tentacles (Herrera et al, 1989), as well as to a greater abundance of gland cells (which have relatively few mitochondria) in other than tentacular tissues. The mitochondrial concentration is also lower in the body wall of M.senile (1.56 X lOlO mitochondria/gwW) than in the entire anemone (4.02 x 1010 mitochondria/gwW), which implies a higher concentration of mitochondria in the tentacles (R.L.…”

In vivo pH measurement at the site of calcification in an octocoral

“…The suspension of SNARF-1 AM-loaded cells was then transferred into a POC cell cultivation chamber (PECON) and cells were allowed to settle on the glass coverslip. After allowing 20 min for cells to load with SNARF-1 AM, the solution overlying the cells was removed by pipetting and replaced with calibration solutions 61 , 62 (Supplementary Material) ranging from pH 6 to 8.5.…”

On the use of 31P NMR for the quantification of hydrosoluble phosphorus-containing compounds in coral host tissues and cultured zooxanthellae

“…All CZ used for the NMR measurements were collected at the same time of day (between 09:00 and 10:00 hrs), and were washed 3 times (centrifugation at 2000 g , for 5 min, at 4 °C) in a buffer (called the “cytoplasmic buffer”) 52 . This buffer contained approximate cytosolic concentrations of major ions in cnidarian cells 53 54 . Phosphatase inhibitors were added to prevent the hydrolysis of organic phosphorus compounds (50 mM NaF, 1 mM Na 3 VO 4 ) and mannitol to adjust osmolarity to 1,100 mOsm l −1 55 .…”

A Functional Biology of Sea Anemones

“…The concentration of mitochondria in the tentacles of A.elegantissima is twice as great as in the anemone as a whole (Table 3.1), and the volume density of mitochondria in tentacular endodermal cells of Aiptasia pulchella is about 3% of cell volume (J.P.S. These relationships may be owing in part to the proportionally smaller mass of essentially acellular mesoglea in the tentacles than in the rest of the anemone, as indicated by a larger extracellular (inulin) space in the body wall than in the tentacles (Herrera et al, 1989), as well as to a greater abundance of gland cells (which have relatively few mitochondria) in other than tentacular tissues. The mitochondrial concentration is also lower in the body wall of M.senile (1.56 X lOlO mitochondria/gwW) than in the entire anemone (4.02 x 1010 mitochondria/gwW), which implies a higher concentration of mitochondria in the tentacles (R.L.…”