Background and purpose: We previously reported that agonist-induced rises in cytoplasmic Ca 2 þ concentration ([Ca 2 þ ] i ) in human umbilical vein endothelial cells (HUVEC) were inhibited after a short-term (2 min) pre-treatment with cAMP-elevating agents. The aim of this work was to study the effects of longer term (8 h) pre-treatment with dibutyryl-cAMP (db-cAMP) or rolipram, a specific inhibitor of phosphodiesterase-4 (PDE4), on [Ca 2 þ ] i , cAMP levels and PDE activity and expression in HUVEC. Experime…
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