Short chain fatty acids enhance expression and activity of the umami taste receptor in enteroendocrine cells via a Gα<sub>i/o</sub>pathway

Shackley, Matilda; Brown, Alastair; Tate, Edward W.; Frost, Gary; Hanyaloglu, Aylin C.

doi:10.1101/2020.06.01.127316

Cited by 2 publications

(1 citation statement)

References 42 publications

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“…Thus, no evidence is available to suppose that an increased expression of TAS1R1 could interfere with piglet appetite. Interestingly, it has been observed that typical bacterial metabolites, butyrate and propionate, can increase mRNA levels of TAS1R1 and TAS1R3 of intestinal EECs altering their sensitivity to glutamate with a mechanism related to the detection by free fatty acid receptors 2 and 3 [45]. The content of volatile fatty acids in the stomach of our piglets was not measured but it is well known that significant amounts of them can be detected in the gastric content of piglets [46].…”

Section: Discussionmentioning

confidence: 79%

Maternal antibiotic treatment affects offspring gastric sensing for umami taste and ghrelin regulation in the pig

Trevisi

Luise

Correa

et al. 2021

J Animal Sci Biotechnol

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Background Scarce is knowledge on the process regulating the development of acid secretion, orexigenic signaling, and chemosensing in the stomach of young pigs. Changes of early microbial encounters by suckling pigs can interact with the gut maturation, by the induction of different molecular signaling. Our goal was to assess if the age of offspring and the maternal environment, influenced by sow antibiotic treatment peripartum, could affect gastric morphology and the expression of genes involved in the control of hydrochloric secretion, feed intake, taste, and inflammation in offspring stomach. Methods 84 pigs from sows fed a diet with amoxicillin (on –d10 to +d21 from farrowing, ANT) or without (CON) were sacrificed at d14, d21, d28 (weaning) or d42. Samples of oxyntic (OXY), pyloric (PY) and cardiac mucosae close to OXY were collected and parietal and enteroendocrine cells (EECs) were counted. Relative gene expression of a set of 11 key genes (ATP4A, SSTR2, GAST, GHRL, MBOAT4, PCSK1, GNAT1, TAS1R1, TAS1R3, IL8 and TNF) was assessed by qRT-PCR. In addition, 40 offspring obtained from the same ANT and CON sows were offered a normal or a fat-enriched diet for 4 weeks between 140 and 169 d of age, and then OXY and PY were sampled. Results The number of parietal and EECs increased with age (P < 0.001). ATP4A increased with age (within suckling, P = 0.043, post-weaning vs. suckling, P < 0.001), SSTR2 increased only after weaning (P < 0.001). In OXY, GHRL increased during suckling (P = 0.012), and post-weaning as a trend (P = 0.088). MBOAT4 tended to increase during suckling (P = 0.062). TAS1R1 increased from suckling to post-weaning period (P =0.001) and was lower in ANT offspring (P = 0.013). GNAT1 in PY was higher in ANT offspring (P = 0.041). Antibiotic treatment of sows peripartum increased expression of GHRL and MBOAT4 in OXY of growing-finishing offspring aged 5 months. Conclusions Data show that sensing for umami taste and ghrelin regulation can be affected by maternal environment, but the development of acid secretion, orexigenic signaling and taste perception in the stomach are mostly developmentally controlled.

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Section: Discussionmentioning

confidence: 79%

Maternal antibiotic treatment affects offspring gastric sensing for umami taste and ghrelin regulation in the pig

Trevisi

Luise

Correa

et al. 2021

J Animal Sci Biotechnol

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Modulation of gut health using probiotics: the role of probiotic effector molecules

Gao

Zhao

Zhang

et al. 2022

Journal of Future Foods

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Short chain fatty acids enhance expression and activity of the umami taste receptor in enteroendocrine cells via a Gα_i/opathway

Abstract: 14The short chain fatty acids (SCFAs) acetate, butyrate and propionate, are produced by the fermentation 15 of non-digestible carbohydrates by the gut microbiota. SCFAs are of interest because they regulate 16 appetite, adiposity, metabolism, glycemic control and immunity.

Cited by 2 publications

References 42 publications

Maternal antibiotic treatment affects offspring gastric sensing for umami taste and ghrelin regulation in the pig

Maternal antibiotic treatment affects offspring gastric sensing for umami taste and ghrelin regulation in the pig

Modulation of gut health using probiotics: the role of probiotic effector molecules

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Short chain fatty acids enhance expression and activity of the umami taste receptor in enteroendocrine cells via a Gαi/opathway

Abstract: 14The short chain fatty acids (SCFAs) acetate, butyrate and propionate, are produced by the fermentation 15 of non-digestible carbohydrates by the gut microbiota. SCFAs are of interest because they regulate 16 appetite, adiposity, metabolism, glycemic control and immunity.

Cited by 2 publications

References 42 publications

Maternal antibiotic treatment affects offspring gastric sensing for umami taste and ghrelin regulation in the pig

Maternal antibiotic treatment affects offspring gastric sensing for umami taste and ghrelin regulation in the pig

Modulation of gut health using probiotics: the role of probiotic effector molecules

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Product

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Short chain fatty acids enhance expression and activity of the umami taste receptor in enteroendocrine cells via a Gα_i/opathway