Shikimic Acid Regulates the NF-κB/MAPK Signaling Pathway and Gut Microbiota to Ameliorate DSS-Induced Ulcerative Colitis

Li, Xin; Mo, Kaibin; Tian, Ge; Zhou, Jiaxin; Gong, J F; Li, Li; Huang, Xianhui

doi:10.1021/acs.jafc.3c00283

Cited by 10 publications

(4 citation statements)

References 37 publications

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“…To further investigate the mechanism of action by which WBP relieves colonic inflammation, we investigated the involvement of the NF-κB and MAPK signaling pathways to inflammation regulation. NF-κB functions as a transcription factor that forms a heterodimer with the p65/p50 subunit and inhibitory protein IκB within the cytoplasm [51]. Upon activation of the signaling pathway, the degradation of IκB proteins enables the translocation of NF-κB dimers into the nucleus, thereby regulating the expression of target genes [52].…”

Section: Discussionmentioning

confidence: 99%

Wheat Bran Polyphenols Ameliorate DSS-Induced Ulcerative Colitis in Mice by Suppressing MAPK/NF-κB Inflammasome Pathways and Regulating Intestinal Microbiota

Wen,

Peng,

Zhang

et al. 2024

Foods

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Wheat bran (WB) is the primary by-product of wheat processing and contains a high concentration of bioactive substances such as polyphenols. This study analyzed the qualitative and quantitative components of polyphenols in wheat bran and their effects on ulcerative colitis (UC) using the dextran sulfate sodium (DSS)-induced colitis model in mice. The potential mechanism of wheat bran polyphenols (WBP) was also examined. Our findings indicate that the main polyphenol constituents of WBP were phenolic acids, including vanillic acid, ferulic acid, caffeic acid, gallic acid, and protocatechuic acid. Furthermore, WBP exerted remarkable protective effects against experimental colitis. This was achieved by reducing the severity of colitis and improving colon morphology. Additionally, WBP suppressed colonic inflammation via upregulation of the anti-inflammatory cytokine IL-10 and downregulation of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) in colon tissues. Mechanistically, WBP ameliorated DSS-induced colitis in mice by inhibiting activation of the MAPK/NF-κB pathway. In addition, microbiome analysis results suggested that WBP modulated the alteration of gut microbiota caused by DSS, with an enhancement in the ratio of Firmicutes/Bacteroidetes and adjustments in the number of Helicobacter, Escherichia-Shigella, Akkermansia, Lactobacillus, Lachnospiraceae_NK4A136_group at the genus level. To conclude, the findings showed that WBP has excellent prospects in reducing colonic inflammation in UC mice.

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Section: Discussionmentioning

confidence: 99%

Wheat Bran Polyphenols Ameliorate DSS-Induced Ulcerative Colitis in Mice by Suppressing MAPK/NF-κB Inflammasome Pathways and Regulating Intestinal Microbiota

Wen,

Peng,

Zhang

et al. 2024

Foods

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“…Pine needles contain essential oils, terpenes, and polyphenols, which promote the absorption and utilization of nutrients in the digestive tract, and the unique aroma of pine needles improves the flavour of the feed and enhances appetite [ 20 ]. Previous studies have reported that proanthocyanidin and shikimic acid in pine needles can control and regulate the expression of cellular pro-inflammatory factors and improve the body's immune response [ 21 , 22 ].…”

Section: Discussionmentioning

confidence: 99%

Supplemental effects of different production methods of pine needle additives on growth performance, intestinal environment, meat quality and serum of broiler chickens

Chang,

Moon,

Wang

et al. 2024

Anim Biosci

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Objective: Pine needles are rich in many nutrients and exhibit antibacterial and antioxidant biological activities; however, the effects of different production methods of pine needle additives on the growth performance and intestinal flora of broiler chickens are not known. Methods: Normal diets were supplemented with PNF (Pine Needle Fermentation juice), PNS (Pine Needle Soaking juice), or PNP (Pine Needle Powder), and the associated effects on growth performance, relative organ weights, intestinal development, intestinal histological morphology, intestinal flora, meat quality, and serum indicators in broiler chickens were observed. Results:The results showed that PNF, PNS, and PNP all significantly improved feed utilisation and promoted the growth and development of broilers. All three additives also significantly improved the structure of the intestinal flora, specifically increasing the diversity of bacteria; increasing the abundance of beneficial bacteria, such as Faecalibacterium, Rikenella, and Blautia; and decreasing the abundance of harmful bacteria, such as Staphylococcus. The antioxidant properties of pine needles were also found to intensify lipid metabolic reactions in the blood, thus leading to lower TG and TCHO. Meanwhile, high doses of PNF reduced jejunum and ileum weights and also increased meat yellowness. Lastly, none of PNF, PNS, or PNP had an effect on relative organ weights or intestinal histological morphology.

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“…When it accumulates to sufficiently high levels, it impairs, restricts, or even kills immune cells [70]. Additionally, shikimic acid demonstrated anti-inflammatory effects [71], and homogentisic acid confers a survival advantage during host infection in Bordetella parapertussis [72].…”

Section: Discussionmentioning

confidence: 99%

Multi-Omics Profiling of Candida albicans Grown on Solid Versus Liquid Media

Alhameed,

Semreen,

Hamad

et al. 2023

Microorganisms

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Candida albicans is a common pathogenic fungus that presents a challenge to healthcare facilities. It can switch between a yeast cell form that diffuses through the bloodstream to colonize internal organs and a filamentous form that penetrates host mucosa. Understanding the pathogen’s strategies for environmental adaptation and, ultimately, survival, is crucial. As a complementary study, herein, a multi-omics analysis was performed using high-resolution timsTOF MS to compare the proteomes and metabolomes of Wild Type (WT) Candida albicans (strain DK318) grown on agar plates versus liquid media. Proteomic analysis revealed a total of 1793 proteins and 15,013 peptides. Out of the 1403 identified proteins, 313 proteins were significantly differentially abundant with a p-value < 0.05. Of these, 156 and 157 proteins were significantly increased in liquid and solid media, respectively. Metabolomics analysis identified 192 metabolites in total. The majority (42/48) of the significantly altered metabolites (p-value 0.05 FDR, FC 1.5), mainly amino acids, were significantly higher in solid media, while only 2 metabolites were significantly higher in liquid media. The combined multi-omics analysis provides insight into adaptative morphological changes supporting Candida albicans’ life cycle and identifies crucial virulence factors during biofilm formation and bloodstream infection.

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Shikimic Acid Regulates the NF-κB/MAPK Signaling Pathway and Gut Microbiota to Ameliorate DSS-Induced Ulcerative Colitis

Cited by 10 publications

References 37 publications

Wheat Bran Polyphenols Ameliorate DSS-Induced Ulcerative Colitis in Mice by Suppressing MAPK/NF-κB Inflammasome Pathways and Regulating Intestinal Microbiota

Wheat Bran Polyphenols Ameliorate DSS-Induced Ulcerative Colitis in Mice by Suppressing MAPK/NF-κB Inflammasome Pathways and Regulating Intestinal Microbiota

Supplemental effects of different production methods of pine needle additives on growth performance, intestinal environment, meat quality and serum of broiler chickens

Multi-Omics Profiling of Candida albicans Grown on Solid Versus Liquid Media

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