Shiga Toxin–producing<i>Escherichia coli</i>Serotype O78:H<sup>–</sup>in Family, Finland, 2009

Lienemann, Taru; Salo, Eeva; Rimhanen-Finne, Ruska; Rönnholm, Kai; Taimisto, Mari; Hirvonen, Jari J.; Tarkka, Eveliina; Kuusi, Markku; Siitonen, Anja

doi:10.3201/eid1804.111310

Cited by 32 publications

(30 citation statements)

References 27 publications

(27 reference statements)

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“…This was in agreement with many previous studies that reported the genetic relatedness between APEC and human ExPEC isolates with an explanation of the origin similarity of both isolates along with the possession of common virulence and antimicrobial resistance genes [22][23][24][25]. Meanwhile, some other studies previously isolated O78 serotypes from stools of diarrheic patients [26][27][28]. Interestingly, the presence of the same serotypes in this study (O26:H11 and O119:H4) isolated from diseased chicken viscera and human stool increases the awareness about the presence of substantial overlap at the level of serogroups between chicken and human isolates.…”

Section: Discussionsupporting

confidence: 81%

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Ramadan

Awad

Ateya

2016

J Infect Dev Ctries

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Introduction: The purpose from this study was to determine phenotypes, intestinal virulence-associated genes, and phylotypic profiling of human diarrheagenic E. coli (DEC) and avian pathogenic E. coli (APEC). Methodology: A total of 108 chicken visceral organs (liver, spleen, heart) from 36 diseased birds (three organs per each bird) and 78 human stool samples (50 diarrheic patients and 28 healthy persons) were randomly collected during the first half of 2015 in the district of Mansoura city, Egypt. Conventional culturing, serotyping, and molecular characterization of virulence genes and phylogroups were performed. Results: Sixty-five (35%) biochemically identified E. coli isolates were detected from chicken visceral (29/108; 26.9%) and human stool samples (36/78; 46.2%). Serotypes O78, O2, and O1 were the most prevalent serotypes (62%) distinguished from APEC isolates, and only two similar serotypes (O119:H4 and O26:H11) were identified from both APEC and DEC isolates. By polymerase chain reaction (PCR), the respective percentages of 100 and 35 with eae and Shiga toxin genes were detected from APEC isolates while 50%, 27.8%, and 19.4% of human DEC isolates harbored eae, stx1, and stx2 genes, respectively. Phylogrouping revealed a significantly higher occurrence of pathogenic phylogroups (D and B2) in APEC (19/29; 65.5%) than in human DEC isolates (8/36; 22.2%). Conclusions: APEC isolates shared serotypes, virulence genes, and phylotypes with human DEC isolates, which is a subsequent potential public health concern. To the best of our knowledge, this is the first report in Egypt that determines virulence gene and phylogroup coexistence between APEC and DEC isolates.

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Section: Discussionsupporting

confidence: 81%

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Ramadan

Awad

Ateya

2016

J Infect Dev Ctries

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“…Reports of bacteremia or sepsis in patients with hemolyticuremic syndrome (HUS) caused by STEC do exist (2,(9)(10)(11). In our study, we found that almost 5% of the strains found in adult patients with bacteremia caused by E. coli carried stx 2 subtypes associated with HUS (12)(13)(14).…”

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confidence: 48%

Extraintestinal Pathogenic Escherichia coli Carrying the Shiga Toxin Gene stx ₂

2013

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“…aEPEC strain UR3457 belongs to phylogroup B2 (ST2018) and serotype O78:H-. Although this serotype is not frequently found in humans, it has also been reported in an STEC isolate recovered from a patient suffering from neonatal bacteremia, bloody diarrhea, and hemolytic-uremic syndrome (HUS) (62). The interconversion between some STEC and aEPEC isolates may arise via the loss and gain of Shiga toxin-coding phages (63,64).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection

et al. 2014

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e Uropathogenic Escherichia coli (UPEC) is the most common cause of community-and hospital-acquired urinary tract infections (UTIs). Isolates from uncomplicated community-acquired UTIs express a variety of virulence traits that promote the efficient colonization of the urinary tract. In contrast, nosocomial UTIs can be caused by E. coli strains that differ in their virulence traits from the community-acquired UTI isolates. UPEC virulence markers are used to distinguish these facultative extraintestinal pathogens, which belong to the intestinal flora of many healthy individuals, from intestinal pathogenic E. coli (IPEC). IPEC is a diarrheagenic pathogen with a characteristic virulence gene set that is absent in UPEC. Here, we characterized 265 isolates from patients with UTIs during inpatient or outpatient treatment at a hospital regarding their phylogenies and IPEC or UPEC virulence traits. Interestingly, 28 of these isolates (10.6%) carried typical IPEC virulence genes that are characteristic of enteroaggregative E. coli (EAEC), Shiga toxin-producing E. coli (STEC), and atypical enteropathogenic E. coli (aEPEC), although IPEC is not considered a uropathogen. Twenty-three isolates harbored the astA gene coding for the EAEC heat-stable enterotoxin 1 (EAST1), and most of them carried virulence genes that are characteristic of UPEC and/or EAEC. Our results indicate that UPEC isolates from hospital patients differ from archetypal community-acquired isolates from uncomplicated UTIs by their spectrum of virulence traits. They represent a diverse group, including EAEC, as well as other IPEC pathotypes, which in addition contain typical UPEC virulence genes. The combination of typical extraintestinal pathogenic E. coli (ExPEC) and IPEC virulence determinants in some isolates demonstrates the marked genome plasticity of E. coli and calls for a reevaluation of the strict pathotype classification of EAEC.

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Shiga Toxin–producingEscherichia coliSerotype O78:H^–in Family, Finland, 2009

Abstract: STEC carrying stx 1c and hlyA genes can invade the human bloodstream.

Cited by 32 publications

References 27 publications

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Extraintestinal Pathogenic Escherichia coli Carrying the Shiga Toxin Gene stx ₂

Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection

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Shiga Toxin–producingEscherichia coliSerotype O78:H–in Family, Finland, 2009

Abstract: STEC carrying stx 1c and hlyA genes can invade the human bloodstream.

Cited by 32 publications

References 27 publications

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Extraintestinal Pathogenic Escherichia coli Carrying the Shiga Toxin Gene stx 2

Characterization of Escherichia coli Isolates from Hospital Inpatients or Outpatients with Urinary Tract Infection

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Shiga Toxin–producingEscherichia coliSerotype O78:H^–in Family, Finland, 2009

Extraintestinal Pathogenic Escherichia coli Carrying the Shiga Toxin Gene stx ₂