Shape-dependent biological activity of spherical and quasi-spherical silver nanoparticles in <i>E. coli</i>, A549 cells and mice

Puchkova, L. V.; Sankova, Tatiana P; Magazenkova, Daria N.; Skomorokhova, Ekaterina A.; Orlov, Iurii A.; Sakhenberg, E. I.; Sosnin, Ilya M.; Farroukh, Mohammad Al; Романов, А. Е.; Ilyechova, Ekaterina Y.

doi:10.1039/d2en00402j

Cited by 4 publications

(3 citation statements)

References 99 publications

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“…Previously, we tested the listed methods of application for their ability to reduce copper status indexes. It was found that IP and IV modes of treatment produced the strongest and almost similar effects, but IP mode was less invasive and thus preferable for chronic application [57].…”

Section: Design Of Agnp Treatment Of Mice With Growing Ascitic or Sol...mentioning

confidence: 99%

Influence of Silver Nanoparticles on the Growth of Ascitic and Solid Ehrlich Adenocarcinoma: Focus on Copper Metabolism

et al. 2023

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The link between copper metabolism and tumor progression motivated us to use copper chelators for suppression of tumor growth. We assume that silver nanoparticles (AgNPs) can be used for lowering bioavailable copper. Our assumption is based on the ability of Ag(I) ions released by AgNPs in biological media and interfere with Cu(I) transport. Intervention of Ag(I) into copper metabolism leads to the replacement of copper by silver in ceruloplasmin and the decrease in bioavailable copper in the bloodstream. To check this assumption, mice with ascitic or solid Ehrlich adenocarcinoma (EAC) were treated with AgNPs using different protocols. Copper status indexes (copper concentration, ceruloplasmin protein level, and oxidase activity) were monitored to assess copper metabolism. The expression of copper-related genes was determined by real-time PCR in the liver and tumors, and copper and silver levels were measured by FAAS. Intraperitoneal AgNPs treatment beginning on the day of tumor inoculation enhanced mice survival, reduced the proliferation of ascitic EAC cells, and suppressed the activity of HIF1α, TNF-α and VEGFa genes. Topical treatment by the AgNPs, which was started together with the implantation of EAC cells in the thigh, also enhanced mice survival, decreased tumor growth, and repressed genes responsible for neovascularization. The advantages of silver-induced copper deficiency over copper chelators are discussed.

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Section: Design Of Agnp Treatment Of Mice With Growing Ascitic or Sol...mentioning

confidence: 99%

Influence of Silver Nanoparticles on the Growth of Ascitic and Solid Ehrlich Adenocarcinoma: Focus on Copper Metabolism

et al. 2023

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“…Nonetheless, they did not come to any conclusions about how particle shape possibly affected the oral toxicity of AgNPs. Given that different antimicrobial activities have been seen in the spherical, quasi-spherical, and octahedral AgNPs, − possibly stemming from their varied facets and surface energy, there is reason to believe that particle shape ought to be responsible for the toxic effects induced by AgNPs in the GIT.…”

Section: Toxicity Mechanisms and Influencing Factors Of Agnps In The Gitmentioning

confidence: 99%

Transformation, Absorption and Toxicological Mechanisms of Silver Nanoparticles in the Gastrointestinal Tract Following Oral Exposure

Wang

Liu

et al. 2023

ACS Nano

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Oral exposure is known as the primary way for silver nanoparticles (AgNPs), which are commonly used as food additives or antibacterial agents in commercial products, to enter the human body. Although the health risk of AgNPs has been a concern and extensively researched over the past few decades, there are still numerous knowledge gaps that need to be filled to disclose what AgNPs experience in the gastrointestinal tract (GIT) and how they cause oral toxicity. In order to gain more insight into the fate of AgNPs in the GIT, the main gastrointestinal transformation of AgNPs, including aggregation/disaggregation, oxidative dissolution, chlorination, sulfuration, and corona formation, is first described. Second, the intestinal absorption of AgNPs is presented to show how AgNPs interact with epithelial cells and cross the intestinal barrier. Then, more importantly, we make an overview of the mechanisms underlying the oral toxicity of AgNPs in light of recent advances as well as the factors affecting the nano−bio interactions in the GIT, which have rarely been thoroughly elaborated in published literature. At last, we emphatically discuss the issues that need to be addressed in the future to answer the question "How does oral exposure to AgNPs cause detrimental effects on the human body?".

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“…Silver nanoparticles are currently demonstrating activity against a wide range of cancer cell lines, including A549, MCF-7, and caco-2, in addition to other types of cancer cell lines [ 9 , 10 ]. This killing efficiency goes back to the small particle size, which facilitates its membrane penetration [ 11 ]. Furthermore, Ag-NPs have recently been discovered to be useful as anti-tumors due to their cytotoxic, antioxidant, and antidiabetic properties, in addition to augmenting the immunogenicity of vaccines and promoting wound and bone healing [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis

Shaaban,

Mohamed,

Zayed

et al. 2024

BMC Biotechnol

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Silver nanoparticles (Ag-NPs) have a unique mode of action as antibacterial agents in addition to their anticancer and antioxidant properties. In this study, microbial nanotechnology is employed to synthesize Ag-NPs using the cell filtrate of Streptomyces enissocaesilis BS1. The synthesized Ag-NPs are confirmed by ultraviolet-visible (UV-Vis), Fourier transform infrared (FT-IR), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Also, the effects of different factors on Ag-NPs synthesis were evaluated to set the optimum synthesis conditions. Also, the antibacterial, antibiofilm, and anticancer activity of Ag-NPs was assessed. The X-ray diffraction (XRD) analysis confirmed the crystalline nature of the sample and validated that the crystal structure under consideration is a face-centered cubic (FCC) pattern. The TEM examination displayed the spherical particles of the Ag-NPs and their average size, which is 32.2 nm. Fourier transform infrared spectroscopy (FTIR) revealed significant changes in functionality after silver nanoparticle dispersion, which could be attributed to the potency of the cell filtrate of Streptomyces enissocaesilis BS1 to act as both a reducing agent and a capping agent. The bioactivity tests showed that our synthesized Ag-NPs exhibited remarkable antibacterial activity against different pathogenic strains. Also, when the preformed biofilms of Pseudomonas aeruginosa ATCC 9027, Salmonella typhi ATCC 12023, Escherichia coli ATCC 8739, and Staphylococcus aureus ATCC 6598 were exposed to Ag NPs 50 mg/ml for 24 hours, the biofilm biomass was reduced by 10.7, 34.6, 34.75, and 39.08%, respectively. Furthermore, the Ag-NPs showed in vitro cancer-specific sensitivity against human breast cancer MCF-7 cell lines and colon cancer cell line Caco-2, and the IC50 was 0.160 mg/mL and 0.156 mg/mL, respectively. The results of this study prove the ease and efficiency of the synthesis of Ag-NPs using actinomycetes and demonstrate the significant potential of these Ag-NPs as anticancer and antibacterial agents.

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Shape-dependent biological activity of spherical and quasi-spherical silver nanoparticles in E. coli, A549 cells and mice

Abstract: Silver nanoparticles (AgNPs) of various size and shape are widely used because of their antibacterial properties. However, their bioactivity to mammals is insufficiently investigated. The aim of the present study...

Cited by 4 publications

References 99 publications

Influence of Silver Nanoparticles on the Growth of Ascitic and Solid Ehrlich Adenocarcinoma: Focus on Copper Metabolism

Influence of Silver Nanoparticles on the Growth of Ascitic and Solid Ehrlich Adenocarcinoma: Focus on Copper Metabolism

Transformation, Absorption and Toxicological Mechanisms of Silver Nanoparticles in the Gastrointestinal Tract Following Oral Exposure

Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis

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