Sex-specific effects of metformin and liraglutide on renal pathology and expression of connexin 45 and pannexin 1 following long-term high-fat high-sugar diet

Luetić, Martina; Kretzschmar, Genia; Grobe, Maximilian; Jerčić, Leo; Bota, Ivana; Ivić, Vedrana; Balog, Marta; Zjalić, Milorad; Uljević, Marija Vitlov; Heffer, Marija; Gáspár, Róbert; Tábi, Tamás; Vukojević, Katarina; Vari, Sandor G.; Filipović, Natalija

doi:10.1016/j.acthis.2021.151817

Cited by 2 publications

(3 citation statements)

References 72 publications

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“…However, Cx45 expression was found in the proximal nephron of rat kidney [ 51 ]. In addition, our previous studies have revealed consistent expression of Cx45 in proximal tubules of mouse and rat kidneys [ 53 , 62 , 63 ].…”

Section: Discussionmentioning

confidence: 65%

A Lack of GD3 Synthase Leads to Impaired Renal Expression of Connexins and Pannexin1 in St8sia1 Knockout Mice

Meter

Racetin

Vukojević

et al. 2022

IJMS

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The aim of this study was to determine the effects of altered ganglioside composition on the expression of Cx37, Cx40, Cx43, Cx45, and Panx1 in different kidney regions of St8sia1 gene knockout mice (St8sia1 KO) lacking the GD3 synthase enzyme. Experiments were performed in twelve male 6-month-old mice: four wild-type (C57BL/6-type, WT) and eight St8sia1 KO mice. After euthanasia, kidney tissue was harvested, embedded in paraffin wax, and processed for immunohistochemistry. The expression of connexins and Panx1 was determined in different regions of the kidney: cortex (CTX.), outer stripe of outer medulla (O.S.), inner stripe of outer medulla (IN.S.), and inner medulla (IN.MED.). We determined significantly lower expression of Cx37, Cx40, Cx45, and Panx1 in different parts of the kidneys of St8sia1 KO mice compared with WT. The most consistent decrease was found in the O.S. where all markers (Cx 37, 40, 45 and Panx1) were disrupted in St8si1 KO mice. In the CTX. region, we observed decrease in the expression of Cx37, Cx45, and Panx1, while reduced expression of Cx37 and Panx1 was more specific to IN.S. The results of the present study suggest that deficiency of GD3 synthase in St8sia1 KO mice leads to disruption of renal Cx expression, which is probably related to alteration of ganglioside composition.

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Section: Discussionmentioning

confidence: 65%

A Lack of GD3 Synthase Leads to Impaired Renal Expression of Connexins and Pannexin1 in St8sia1 Knockout Mice

Meter

Racetin

Vukojević

et al. 2022

IJMS

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“…One report demonstrated that genetic ablation of Panx1 aids in ischemic recovery in female but not male mice (Freitas‐Andrade et al, 2017). Additionally, renal expression of Panx1 in high‐fat high‐sugar fed mice is differentially regulated in female and males following metformin treatment (Luetić et al, 2021). Furthermore, Aguilar‐Perez and colleagues reported that osteocytic Panx1 deletion resulted in increased muscle mass and unaltered strength in female mice (Aguilar‐Perez et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…*p < 0.05. ANOVA, analysis of variance; DAPI, 4′,6-diamidino-2-phenylindole; ns, non-significant; SD, standard deviation metformin treatment (Luetić et al, 2021). Furthermore, Aguilar-Perez and colleagues reported that osteocytic Panx1 deletion resulted in increased muscle mass and unaltered strength in female mice (Aguilar-Perez et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Sex‐dependent role of Pannexin 1 in regulating skeletal muscle and satellite cell function

Freeman

Langlois

Scott

et al. 2022

Journal Cellular Physiology

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The development and regeneration of skeletal muscle are mediated by satellite cells (SCs), which ensure the efficient formation of myofibers while repopulating the niche that allows muscle repair following injuries. Pannexin 1 (Panx1) channels are expressed in SCs and their levels increase during differentiation in vitro, as well as during skeletal muscle development and regeneration in vivo. Panx1 has recently been shown to regulate muscle regeneration by promoting bleb‐based myoblast migration and fusion. While skeletal muscle is largely influenced in a sex‐specific way, the sex‐dependent roles of Panx1 in regulating skeletal muscle and SC function remain to be investigated. Here, using global Panx1 knockout (KO) mice, we demonstrate that Panx1 loss reduces muscle fiber size and strength, decreases SC number, and alters early SC differentiation and myoblast fusion in male, but not in female mice. Interestingly, while both male and female Panx1 KO mice display an increase in the number of regenerating fibers following acute injury, the newly formed fibers in male Panx1 KO mice are smaller. Overall, our results demonstrate that Panx1 plays a significant role in regulating muscle development, regeneration, and SC number and function in male mice and reveal distinct sex‐dependent functions of Panx1 in skeletal muscle.

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Sex-specific effects of metformin and liraglutide on renal pathology and expression of connexin 45 and pannexin 1 following long-term high-fat high-sugar diet

Cited by 2 publications

References 72 publications

A Lack of GD3 Synthase Leads to Impaired Renal Expression of Connexins and Pannexin1 in St8sia1 Knockout Mice

A Lack of GD3 Synthase Leads to Impaired Renal Expression of Connexins and Pannexin1 in St8sia1 Knockout Mice

Sex‐dependent role of Pannexin 1 in regulating skeletal muscle and satellite cell function

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