Sex-related effects of agmatine on caffeine-induced locomotor activity in Swiss Webster mice

Uzbay, Tayfun; Kose, Akin; Kayır, Hakan; Ulusoy, Kemal Gokhan; Çelik, Turgay

doi:10.1016/j.ejphar.2009.12.020

Cited by 14 publications

(4 citation statements)

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“…However, no sex-related differences in caffeine-induced LMA increase have been found. 42 Discrepancy might also arise from the different light/dark cycles in which the two behavioral experiments were performed. In order to favor the higher levels of LMA associated with the dark (active) phase of the animal’s activity cycle, our experiments were conducted under dark conditions, while the Stevenson et al’s 11 study was conducted under light conditions.…”

Section: Discussionmentioning

confidence: 99%

Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice

Puente

Romero-Alejo

Vela

et al. 2015

JPR

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Reflex-based procedures are important measures in preclinical pain studies that evaluate stimulated behaviors. These procedures, however, are insufficient to capture the complexity of the pain experience, which is often associated with the depression of several innate behaviors. While recent studies have made efforts to evidence the suppression of some positively motivated behaviors in certain pain models, they are still far from being routinely used as readouts for analgesic screening. Here, we characterized and compared the effect of the analgesic ibuprofen (Ibu) and the stimulant, caffeine, in assays of acute pain-stimulated and pain-depressed behavior. Intraperitoneal injection of acetic acid (AA) served as a noxious stimulus to stimulate a writhing response or depress saccharin preference and locomotor activity (LMA) in mice. AA injection caused the maximum number of writhes between 5 and 20 minutes after administration, and writhing almost disappeared 1 hour later. AA-treated mice showed signs of depression-like behaviors after writhing resolution, as evidenced by reduced locomotion and saccharin preference for at least 4 and 6 hours, respectively. Depression-like behaviors resolved within 24 hours after AA administration. A dose of Ibu (40 mg/kg) – inactive to reduce AA-induced abdominal writhing – administered before or after AA injection significantly reverted pain-induced saccharin preference deficit. The same dose of Ibu also significantly reverted the AA-depressed LMA, but only when it was administered after AA injection. Caffeine restored locomotion – but not saccharin preference – in AA-treated mice, thus suggesting that the reduction in saccharin preference – but not in locomotion – was specifically sensitive to analgesics. In conclusion, AA-induced acute pain attenuated saccharin preference and LMA beyond the resolution of writhing behavior, and the changes in the expression of hedonic behavior, such as sweet taste preference, can be used as a more sensitive and translational model to evaluate analgesics.

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Section: Discussionmentioning

confidence: 99%

Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice

Puente

Romero-Alejo

Vela

et al. 2015

JPR

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“…Uzbay et al [23] reported the absence of sex-differences with caffeine administration in mice, although a sex-differential response was seen with agmatine on caffeine-induced locomotor response. In an earlier study [21], we reported that male mice (more than females) showed an early locomotor response, although females showed higher basal levels of locomotion.…”

Section: Introductionmentioning

confidence: 99%

“…The existence of sex differences in the response to caffeine in young subjects have been studied in humans [8], [20] and rodents [21], [22], [23], with different conclusions. Soellner et al [24] reported improved object-discrimination in female rats, following caffeine ingestion.…”

Section: Introductionmentioning

confidence: 99%

Caffeine and sleep-deprivation mediated changes in open-field behaviours, stress response and antioxidant status in mice

Onaolapo

Akanmu

et al. 2016

Sleep Science

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ObjectivesEffects of daily caffeine consumption on open-field behaviours, serum corticosterone and brain antioxidant levels were investigated after six hours of total sleep-deprivation in prepubertal mice. We tested the hypothesis that daily caffeine consumption may significantly alter behaviour, stress and antioxidative response of prepubertal mice to an acute episode of total sleep-deprivation.MethodsPrepubertal Swiss mice of both sexes were assigned to two main groups of 120 each (subdivided into 6 groups of 10 each, based on sex), and administered vehicle or graded oral doses of caffeine (10, 20, 40, 80 and 120 mg/kg/day) for 14 days. On day 14, a main group was subjected to 6 h of total sleep-deprivation by ‘gentle-handling’. Open-field behaviours were then assessed in both groups, after which animals were euthanized, and levels of corticosterone, superoxide dismutase and glutathione peroxidase assayed.ResultsHorizontal locomotion, rearing and grooming increased significantly, compared to control, with sleep-deprived (SD) mice showing stronger caffeine-driven responses at higher doses; and SD female mice showing sustained response to caffeine, compared to respective males. Plasma corticosterone increased with increasing doses of caffeine in both non sleep-deprived (NSD) and SD mice; although SD mice had higher corticosterone levels. Sleep-deprivation and/or higher doses of caffeine were associated with derangements in brain antioxidant levels.ConclusionRepeated caffeine consumption and/or acute sleep-deprivation led to significant changes in pattern of open-field behaviour and stress/antioxidant response in mice. Responses seen in the study are probably due to modulatory effects of caffeine on the total body response to stressful stimuli.

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“…The recommended dosages outlined in the ISO and AGM literature were administered i.p. [11,12]. To conclude the experiment, rats were anesthetized with 1.2 g/kg of ethyl carbamate (also referred to as urethane) i.p.…”

Section: Methodsmentioning

confidence: 99%

The protective and therapeutic effects of agmatine on isoproterenol-induced myocardial injury in rats

Ulutas,

Tapsiz,

Ozhan

et al. 2023

Med-Science

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Chronic inflammation and oxidative stress can contribute to the development of cardiovascular diseases. Isoproterenol (ISO), a synthetic catecholamine, is used to study the effects of drugs on cardiotoxicity. Agmatine (AGM) is a type of biogenic amine produced through the decarboxylation of arginine. The purpose of the current study was to evaluate the effects of AGM against ISO-induced cardiotoxicity due to the described roles of AGM in cardiovascular disease. Four groups of thirty-two Wistar Albino rats were divided equally as control, ISO, AGM+ISO, and ISO+AGM. ISO was administered intraperitoneally (i.p.) twice at a dose of 150 mg/kg, at 24-hour intervals. Prior to and after ISO injection, 20 mg/kg of AGM was injected i.p. Hemodynamic measurements and serum and tissue biochemical analyses were evaluated at the end of the experiment. The levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) in the tissue were measured. In the ISO group, levels of lactate dehydrogenase (LDH) and creatine kinase (CK) were increased significantly (p<0.05). Co-administration of ISO and AGM significantly reduced CK and LDH levels (p<0.05). MDA levels increased in the ISO-treated group but decreased in the AGM-treated groups (p<0.05). Notably, there was a reduction in the CAT level in the ISO treatment group (p<0.05). CAT was found to be significantly increased (p<0.05) in the groups that received AGM compared to the ISO group. AGM+ISO group had a reduced density of degenerated cardiomyocytes and granulation tissue compared to the ISO group (p<0.05). Although granulation tissue demonstrated a significant reduction in the ISO+AGM group in comparison to the ISO group (p<0.05). In this study, the results indicate that AGM treatment can potentially inhibit ISO-induced myocardial injury and vascular dysfunction by preserving vascular integrity. Notably, the protective effects of AGM on cardiac damage appear to outweigh its therapeutic benefits, as shown by histopathological analysis

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Sex-related effects of agmatine on caffeine-induced locomotor activity in Swiss Webster mice

Cited by 14 publications

References 50 publications

Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice

Changes in saccharin preference behavior as a primary outcome to evaluate pain and analgesia in acetic acid-induced visceral pain in mice

Caffeine and sleep-deprivation mediated changes in open-field behaviours, stress response and antioxidant status in mice

The protective and therapeutic effects of agmatine on isoproterenol-induced myocardial injury in rats

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