Sex chromosome complement and developmental diversity in pre-and post-hatching porcine embryos

Cassar, Glen; Fuente, Rabindranath De La; Yu, Zhaoning; King, G. J.; King, W.A.

doi:10.1016/0093-691x(95)00277-f

Cited by 11 publications

(5 citation statements)

References 23 publications

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“…In view of the importance of SOX2 levels for the maintenance of stem cell potential in vitro and for making early lineage fate decisions in vivo (Fong et al, 2008; Kopp et al, 2008), our data raise the possibility that the transcriptional activity of the closely related SRY within cells of the inner cell mass of preimplantation male embryos may influence embryo development in a sexually dimorphic manner. This possibility is supported by our semiquantitative RT‐PCR data and may provide a molecular explanation for the observations that preimplantation male embryos in several mammalian species are developmentally more advanced compared with their female counterparts (Tsunoda et al, 1985; Xu et al, 1992; Pergament and et al, 1994; Cassar et al, 1995; Bernardi and Delouis, 1996). All SOX proteins including SRY recognize the same core DNA binding motif.…”

Section: Discussionsupporting

confidence: 73%

Transgenic mouse analysis of Sry expression during the pre‐ and peri‐implantation stage

Silversides¹,

Raiwet²,

Souchkova³

et al. 2012

Developmental Dynamics

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Background: The SRY/Sry gene is expressed in pre-Sertoli cells of the male genital ridge and functions as the mammalian testis determining factor (TDF). In addition, expression of SRY/Sry outside the genital ridge has been reported, including preimplantation embryos, although the functional significance of this is not well understood. Results: Using Cre-mediated lineage studies and transgenic reporter mouse models, we now show that promoter sequences of human, pig and mouse SRY drive robust reporter gene expression in epiblast cells of peri-implantation embryos between embryonic day (E) 4.5 and E6.5. Analysis of endogenous Sry expression revealed that linear transcripts are produced by means of multiple polyadenylation sites in E4.5 embryos. Within the epiblast, SRY reporter expression mimics the expression seen using a Gata4 reporter model, but is dissimilar to that seen using an Oct4 reporter model. In addition, we report that overexpression of mouse Sry in embryonic stem cells leads to down-regulation of the core pluripotency markers Sox2 and Nanog. Conclusion: We propose that SRY/Sry may function as a male-specific maturation factor in the peri-implantation mammalian embryo, providing a genetic mechanism to help explain the observation that male embryos are developmentally more advanced compared with female embryos, and suggesting a role for SRY beyond that of TDF. Developmental Dynamics 241:1192-1204, 2012. V C 2012 Wiley Periodicals, Inc.Key words: SRY; promoter; blastocyst; epiblast; male-specific maturation factor; pluripotency; Sox2; Oct4; Nanog Key findings:The proximal promoter of SRY/Sry from several mammalian species (pig, human and mouse) directs reporter gene expression in the epiblast of pre-and peri-implantation mouse embryos. Expression levels of SRY/Sry reporters coincide with the maturation stage of pre-and peri-implantation embryos, being positively correlated with a Gata4 reporter and negatively correlated with an Oct4 reporter. Overexpression of Sry in murine embryonic stem cells results in reduced expression of the pluripotency markers Sox2 and Nanog.

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Section: Discussionsupporting

confidence: 73%

Transgenic mouse analysis of Sry expression during the pre‐ and peri‐implantation stage

Silversides¹,

Raiwet²,

Souchkova³

et al. 2012

Developmental Dynamics

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“…Although the animal data supporting increased developmental rates in male embryos are strong (14)(15)(16)(17)(18)(19)(20)(21), human data are limited. Tarin et al (35) calculated the difference in mean cell number between transferred embryos and nontransferred embryos.…”

Section: Discussionmentioning

confidence: 95%

Influence of embryo sex on development to the blastocyst stage and euploidy

Eaton¹,

Hacker²,

Barrett³

et al. 2011

Fertility and Sterility

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“…However, the sex related differential growth rate during the preimplantation period probably must be considered to be an in vitro artefact as it has been shown that male and female embryos develop at different rates in vitro but not in vivo in the mouse (Peippo and Bredbacka, 1995), bovine (Gutiérrez-Adán et al, 1996, and pig (Kaminski et al, 1996). Not all reported data indicate however that the sex-related growth differences are an in vitro artefact: in the mouse (Burgoyne, 1993) and in the pig (Cassar et al, 1995) also in vivo derived male blastocysts had significantly more cells as compared to female embryos. Recently, it was shown that in in vitro-cultured bovine embryos, female blastocysts produced double the amount of G6PD transcripts compared with male blastocysts, while in in vivo-produced embryos, female and male blastocysts produced equal amounts of G6PD (Wrenzycki et al, 2002), again an indication that sex-related embryonic differences are an in vitro artefact.…”

Section: Discussionmentioning

confidence: 99%

Growth rate of human preimplantation embryos is sex dependent after ICSI but not after IVF

Dumoulin¹,

Derhaag²,

Bras³

et al. 2005

Human Reproduction

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Sex chromosome complement and developmental diversity in pre-and post-hatching porcine embryos

Cited by 11 publications

References 23 publications

Transgenic mouse analysis of Sry expression during the pre‐ and peri‐implantation stage

Transgenic mouse analysis of Sry expression during the pre‐ and peri‐implantation stage

Influence of embryo sex on development to the blastocyst stage and euploidy

Growth rate of human preimplantation embryos is sex dependent after ICSI but not after IVF

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