Severity of thermal burn injury is associated with systemic neutrophil activation

Laggner, Maria; Lingitz, Marie-Therese; Copic, Dragan; Direder, Martin; Klas, Katharina; Bormann, Daniel; Gugerell, Alfred; Moser, Bernhard; Radtke, Christine; Hacker, Stefan; Mildner, Michael; Ankersmit, Hendrik Jan; Haider, Thomas

doi:10.1101/2021.10.07.21264679

Cited by 5 publications

(7 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, in the current research, WBC was suggested to form an independent risk factor for determining the severity of SII, ARDS development in patients with SII and SII mortality. Consistent with our previous study that the severity of thermal burn injury is associated with WBC activation, WBC and neutrophil counts were significantly increased on admission day (40). Compared with the other indicators in severity of SII group, the highest AUC and sensitivity were 0.774 and 75.0%, respectively.…”

Section: Univariate Logistic Regression Multivariate Logistic Regressionsupporting

confidence: 90%

Clinical characteristics and predictors of burn complicated with smoke inhalation injury: A retrospective analysis

et al. 2022

View full text Add to dashboard Cite

Fire smoke enters the human lungs through the respiratory tract. The damage to the respiratory tract and lung tissue is known as smoke inhalation injury (SII). Fire smoke can irritate airway epithelium cells, weaken endothelial cell adhesion and lyse alveolar type II epithelia cells, leading to emphysema, decreased lung function, pneumonia and risk of acute lung injury/acute respiratory distress syndrome (ARDS). The purpose of the present study was to analyze the clinical characteristics of patients with SII and the risk factors affecting their prognosis. A total of 103 patients with SII admitted between January 2016 to December 2021 to the Burns Unit of the Characteristic Medical Center of Chinese People's Armed Police Force and 983 Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army were selected for the present study. The demographics and clinical features between different severities of SII were analyzed. Univariate/multivariate logistic regression was used to analyze the potential predictors for severity, ARDS and mortality of patients with SII. Receiver operating characteristic (ROC) curves were used to screen independent risk factors and identify their prediction accuracy. It was concluded that total body surface area (TBSA), III burn area (of total %TBSA), cases of respiratory infections, ARDS morbidity, mortality, acute physiology and chronic health evaluation II, lung injury prediction score, lactic acid, white blood cells (WBC), alanine transaminase, blood urea nitrogen, serum creatinine and uric acid were indicators that were raised with increasing severity of SII. However red blood cells, hemoglobin, platelet count, total protein, albumin, and albumin/globulin were decreased with the increasing severity of SII (P<0.05). WBC >20.91 (10 9 /l) was a reliable indicator for severe SII. Lactic acid >9.60 (mmol/l) demonstrated a high degree of accuracy in predicting ARDS development in patients with SII. Hemoglobin <83.00 (g/l) showed a high degree of accuracy in predicting mortality. In summary, the highlighted assessment parameters could be used to contribute to devising improved treatment plans to preempt worsening conditions (such as shock, ARDS, multiple organ dysfunction syndrome and death).

show abstract

Section: Univariate Logistic Regression Multivariate Logistic Regressionsupporting

confidence: 90%

Clinical characteristics and predictors of burn complicated with smoke inhalation injury: A retrospective analysis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…We also found increased CitH3 and NE concentrations following severe trauma, whereas these differences were not stated as signi cant. Interestingly, measured serum levels of MPO, CitH3, and NE were lower than those in patients suffering from burn injuries 22 . These results indicate a lower induction of NETosis in polytraumatized patients compared with burn injury patients.…”

Section: Discussionmentioning

confidence: 77%

“…Previously, we were able to demonstrate systemic neutrophil activation after burn injury 22 . This is in accord with other studies reporting a role for neutrophils and NETs in burn injury 27 ,, ,28 ,critically ill patients 21 , sepsis 29 , and lung injury 30 .…”

Section: Introductionmentioning

confidence: 93%

“…Neutrophils play a major role as the rst line of innate immune defense and are the most abundant leukocyte in humans, constituting 60-70% of circulating leukocytes 9,[19][20][21] . Neutrophils exert several antimicrobial mechanisms, including phagocytosis, release of effector molecules, and formation of neutrophil extracellular traps (NETs) 22,23 . NETs were rst described by Brinkman et al in 2004 24 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Elevation of neutrophil-derived factors in patients after multiple trauma

Lingitz

Wollner

Bauer

et al. 2022

Preprint

View full text Add to dashboard Cite

Trauma represents one of the leading causes of death worldwide. Traumatic injuries elicit a dynamic inflammatory response with systemic release of inflammatory cytokines. Disbalance of this response can lead to systemic inflammatory response syndrome or compensatory anti-inflammatory response syndrome. As neutrophils play a major role in innate immune defense and are crucial in the injury-induced immunological response, we aimed to investigate systemic neutrophil-derived immunomodulators in trauma patients. Therefore, serum levels of neutrophil elastase (NE), myeloperoxidase (MPO), and citrullinated histone H3 (CitH3) were quantified in patients with injury severity scores above 15. Additionally, leukocyte, platelet, fibrinogen, and CRP levels were assessed. Lastly, we analyzed the association of neutrophil-derived factors with clinical severity scoring systems. Although the release of MPO, NE, and CitH3 was not predictive of mortality, we found a remarkable increase in MPO and NE in trauma patients as compared with healthy controls. We also found significantly increased levels of MPO and NE on days 1 and 5 after initial trauma in critically injured patients. Taken together, our data suggest a role for neutrophil activation and NETosis in trauma. Targeting exacerbated neutrophil activation might represent a new therapeutic option for critically injured patients.

show abstract

“…It is worth noting that we detected different expression of the same gene in the blood and skin at different burn times. In the process of severe burn, there are a series of immune reactions such as inflammation (20). This shows that the same reaction mechanism is present in different burn times.…”

mentioning

confidence: 96%

Molecular imbalance mechanism of skin and blood leucocytes in severe burn patients at different burn times

Liang¹,

Qiu²,

Wu³

et al. 2022

Ann Transl Med

View full text Add to dashboard Cite

Background: Severe burns are a leading cause of injuries worldwide and are usually accompanied by considerable morbidity and mortality. The purpose of this study was to investigate the changes of gene expression in blood and skin at different times after severe burn.Methods: Firstly, the gene expression profiles of different burn time samples in GSE19743 and GSE8056 were analyzed. Secondly, the maladjusted gene network was identified by protein-protein interaction (PPI) network, and the genes in the network were enriched and analyzed. In addition, the key dysfunctional genes were identified by betweenness algorithm, and evaluated by survival analysis, Cox analysis, receiver operating characteristic (ROC) analysis. Finally, crosstalk analysis and enrichment analysis were carried out between the blood-and skin-specific differentially expressed genes (DEGs) at different burn times. Results:The results showed that there were common DEGs in the blood and skin at different burn times.Importantly, we screened out the key dysfunctional genes BIRC5, NCAM1, PCNA, TOP2A, and VEGFA, which were related to the course of burns. Enrichment analysis showed that these maladjusted genes were mainly involved in the immune inflammation-related signal pathway. Additionally, significant crosstalk was identified between blood-and skin-specific genes at different burn times, especially in the blood. The signal pathways involved in specific genes represent their own pathological characteristics.Conclusions: Both blood and skin tissues express common pathological changes and unique molecular mechanisms at different times after burn injury. The results of this study provide guidance for clinical personalized treatment.

show abstract

Severity of thermal burn injury is associated with systemic neutrophil activation

Cited by 5 publications

References 53 publications

Clinical characteristics and predictors of burn complicated with smoke inhalation injury: A retrospective analysis

Clinical characteristics and predictors of burn complicated with smoke inhalation injury: A retrospective analysis

Elevation of neutrophil-derived factors in patients after multiple trauma

Molecular imbalance mechanism of skin and blood leucocytes in severe burn patients at different burn times

Contact Info

Product

Resources

About