Serum uric acid determined by reversed-phase liquid chromatography with spectrophotometric detection.

Kiser, Ernest J.; Johnson, Gina; Witte, David L.

doi:10.1093/clinchem/24.4.536

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1981

2002

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Cited by 14 publications

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“…The residues were reconstituted with 0.3 mL of 20 M NaOH and filtered through 0.45-µ Aero LC-13 filters with 1-mL disposable syringes. Ten microliters (7400 dpm) of each sample was applied to reversed-phase TLC plates and chromatographed in closed TLC tanks containing 100 mL of 10 mM sodium acetate buffer (pH 7.0) (Kiser et al, 1978). This procedure separated [14C]xanthine from [14C]uric acid.…”

Section: Methodsmentioning

confidence: 99%

Absorption of enzymically active iodine-125-labeled bovine milk xanthine oxidase fed to rabbits

Rzucidlo¹,

Zikakis²

1990

J. Agric. Food Chem.

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Section: Methodsmentioning

confidence: 99%

Absorption of enzymically active iodine-125-labeled bovine milk xanthine oxidase fed to rabbits

Rzucidlo¹,

Zikakis²

1990

J. Agric. Food Chem.

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Determination of uric acid in human serum by capillary electrophoresis with polarity reversal and electrochemical detection

Boughton¹,

Robinson²,

Strein³

2002

Electrophoresis

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Capillary zone electrophoresis (CE) under conditions of reversed polarity is used in conjunction with electrochemical detection (EC) at carbon fiber microcylinder electrodes for the selective and sensitive determination of uric acid in human blood serum. Comigration of anions with the electroosmotic flow is accomplished with reversed polarity and the buffer additive cetyltrimethylammonium bromide (CTAB) in a 2-(N-morpholino)ethanesulfonic acid (MES) buffer system, giving rise to rapid and sensitive analyses. Optimal buffer conditions (pH 7.0), detection potential (0.80 V vs. Ag/AgCl), and electrokinetic injection are employed to allow for maximal resolution and signal intensity. Amperometric end-column detection with a carbon fiber microcylinder electrode results in lower limits of detection for uric acid of about 25 nM (ca. 140 amol injected) without the need for decoupling. Linear calibration plots using uric acid standards in water and serum are obtained over a linear range from 5.00 x 10(-4) M to 2.50 x 10(-7) M. Uric acid concentrations obtained for human sera using the CE-EC approach described here are shown to compare favorably to the accepted laboratory values.

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1981

Arthritis & Rheumatism

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Serum uric acid determined by reversed-phase liquid chromatography with spectrophotometric detection.

Cited by 14 publications

References 0 publications

Absorption of enzymically active iodine-125-labeled bovine milk xanthine oxidase fed to rabbits

Absorption of enzymically active iodine-125-labeled bovine milk xanthine oxidase fed to rabbits

Determination of uric acid in human serum by capillary electrophoresis with polarity reversal and electrochemical detection

Experimental models

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